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<h4>STEP 2: Create Reporter Plasmid </h4> | <h4>STEP 2: Create Reporter Plasmid </h4> | ||
− | <h4>STEP 3 : Create Promoter Library</h4> | + | <h4>STEP 3: Create Promoter Library</h4> |
− | <h4>STEP 4 : Random Ligations </h4> | + | <h4>STEP 4: Random Ligations </h4> |
Revision as of 18:42, 29 October 2017
RESULTS:
STEP 1: Create guideRNA Plasmid
STEP 2: Create Reporter Plasmid
STEP 3: Create Promoter Library
STEP 4: Random Ligations
STEP 5: Freeze drying & Revivial
STEP 6: CRISPRi & guideRNA efficiency
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.