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<p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, | <p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, | ||
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− | <label for="question-2" | + | <label for="question-2"> STEP 2: Create Reporter Plasmid </label> |
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− | <label for="question-3"> | + | <label for="question-3"> STEP 3: Create Promoter Library </label> |
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<input type="checkbox" id="question-4"> | <input type="checkbox" id="question-4"> | ||
− | <label for="question-4" | + | <label for="question-4">STEP 4: Random Ligations </label> |
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+ | <label for="question-5">STEP 5: Freeze drying & Revivial </label> | ||
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+ | <input type="checkbox" id="question-6"> | ||
+ | <label for="question-6"> STEP 6: CRISPRi & guideRNA efficiency </label> | ||
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Revision as of 20:27, 29 October 2017
RESULTS:
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.
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