Introduction

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Nitrate is toxic to fish or shrimp in water. When nitrate reach a certain concentration level, aquatic animal will start to die. So, in order to maintain good quality of water, concentration of nitrate in water need to be tested every day. In corporate to our team’s project “NO PROBLEM” this year, decide to sense the concentration of nitrite and nitrate as a part of our project.

Description

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Part: BBa_K381001 was first designed by Katharine Coyte from team BCCS-Bristol in iGEM 2010. It is a Nitrate reporter, PyeaR - GFP composite. The team BCCS-Bristol only test the BioBrick’s sensitivity at a higher concentration, but the Nitrate level of aquatic water usually won’t reach 1 mM. Therefore, we carry out experiment by testing the fluorescence intensity with ppm level of Potassium Nitrate, which is much lower than all the teams before. The results are shown below.

It shows that the BioBrick BBa_K381001 have nice sensitivity not just in high Nitrate concentration, but low as well. Many teams had done improvements of K381001, but our team bring out the most beautiful data. And we also take X axis logarithm, which can make our data become linear. Not only easier to read, but also more meaningful.

The data is shown as below.

ppm conc	8 hours	12 hours	24 hours
0	454.623	546.841	620.402
2	689.961	754.085	821.113
4	766.835	866.846	922.813
6	816.472	916.119	972.653
8	831.843	931.43	985.14
10	900.114	998.935	1058.844
20	962.493	1093.076	1148.216
40	1090.926	1200.976	1252.106
60	1129.622	1253.052	1295.002
80	1240.331	1341.711	1373.601
100	1259.766	1385.946	1449.946