Difference between revisions of "Team:UNebraska-Lincoln/Basic Part"

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<p>Vanadium dependent bromoperoxidase is a kind of haloperoxidase that is involved in the bromination of organic halo-compounds associated with defense and pigmentation in seaweeds and marine algae. The active site features a vanadium oxide center attached to the protein via one histidine side chain and a collection of hydrogen bonds to the oxide ligands. The enzyme can use many different hydrocarbon substrates to catalyse the oxidation of bromide by hydrogen peroxide. The resulting electrophilic bromonium cation (Br+) attacks hydrocarbons. This gene was utilized in our composite part (BBa_k2461000) for its ability to create bromoform which has been proven to reduce methane in cattle as described in full <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Description#whatToDo">HERE</a>.</p>
 
<p>Vanadium dependent bromoperoxidase is a kind of haloperoxidase that is involved in the bromination of organic halo-compounds associated with defense and pigmentation in seaweeds and marine algae. The active site features a vanadium oxide center attached to the protein via one histidine side chain and a collection of hydrogen bonds to the oxide ligands. The enzyme can use many different hydrocarbon substrates to catalyse the oxidation of bromide by hydrogen peroxide. The resulting electrophilic bromonium cation (Br+) attacks hydrocarbons. This gene was utilized in our composite part (BBa_k2461000) for its ability to create bromoform which has been proven to reduce methane in cattle as described in full <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Description#whatToDo">HERE</a>.</p>
  
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2461001"><h3 class=leap>BBa_k2461001 NrfA:</h3></a>
  
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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<p>This gene encodes an enzyme responsible for the reduction of nitrite to ammonia without releasing intermediate products. This catalytic reaction occurs within the periplasmic space of gram-negative bacteria. Either hydrogen, formate, or sulfide can be used as an electron donor. This part was used in conjunction with (BBa_K2461003) to reduce nitrite to ammonia within the rumen of cattle. More information can be found <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Description#whatToDo">HERE</a>.</p>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<h3>Best Basic Part Special Prize</h3>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2461003"><h3 class=leap>BBa_k2461003 NrfH:</h3></a>
  
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<p>This gene encodes an enzyme subunit for cytochrome c552. It acts as an inner membrane anchor for the partnering unit and helps mediate electron transport. Cytochrome c552's ability would be impaired without this subunit.This part was used in conjunction with (BBa_K2461001) to reduce nitrite to ammonia within the rumen of cattle. More information can be found <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Description#whatToDo">HERE</a>.</p>
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<h4>Note</h4>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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Revision as of 14:43, 30 October 2017

UNL 2017

Helping reduce methane emissions from livestock

Team Collaborations
Description Design Experiments Notebook InterLab Contribution Model Results Attributions
Parts Basic Parts Composite Parts
Safety
Silver HP Integrated and Gold

Basic Parts

BBa_k2461002 Vanadium-Dependent Bromoperoxidase:

Vanadium dependent bromoperoxidase is a kind of haloperoxidase that is involved in the bromination of organic halo-compounds associated with defense and pigmentation in seaweeds and marine algae. The active site features a vanadium oxide center attached to the protein via one histidine side chain and a collection of hydrogen bonds to the oxide ligands. The enzyme can use many different hydrocarbon substrates to catalyse the oxidation of bromide by hydrogen peroxide. The resulting electrophilic bromonium cation (Br+) attacks hydrocarbons. This gene was utilized in our composite part (BBa_k2461000) for its ability to create bromoform which has been proven to reduce methane in cattle as described in full HERE.

BBa_k2461001 NrfA:

This gene encodes an enzyme responsible for the reduction of nitrite to ammonia without releasing intermediate products. This catalytic reaction occurs within the periplasmic space of gram-negative bacteria. Either hydrogen, formate, or sulfide can be used as an electron donor. This part was used in conjunction with (BBa_K2461003) to reduce nitrite to ammonia within the rumen of cattle. More information can be found HERE.

BBa_k2461003 NrfH:

This gene encodes an enzyme subunit for cytochrome c552. It acts as an inner membrane anchor for the partnering unit and helps mediate electron transport. Cytochrome c552's ability would be impaired without this subunit.This part was used in conjunction with (BBa_K2461001) to reduce nitrite to ammonia within the rumen of cattle. More information can be found HERE.