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<p>At the beginning of our project, the iGEM part library was checked for parts that could be used for the <a href="https://2017.igem.org/Team:Freiburg/Design">CARTEL<sup>TM</sup> AND gate</a>. Since oxygen concentration plays a key role in the tumor microenvironment, we were especially interested in parts containing HREs (Hypoxia Response Elements). Though, since those BioBricks only covered the needs of our project partially, we decided to improve <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1720002" target= " _blank">BBa_K1720002</a> (iGEM15_SCUT-China) and <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1456004" target= " _blank">BBa_K1456004</a> (iGEM14_ATOMS-Turkiye). So we sent in <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2295003" target= " _blank">BBa_K2295003</a>, to fulfill the needs further iGEM teams, dealing with hypoxia dependent gene expression, might have. </p> | <p>At the beginning of our project, the iGEM part library was checked for parts that could be used for the <a href="https://2017.igem.org/Team:Freiburg/Design">CARTEL<sup>TM</sup> AND gate</a>. Since oxygen concentration plays a key role in the tumor microenvironment, we were especially interested in parts containing HREs (Hypoxia Response Elements). Though, since those BioBricks only covered the needs of our project partially, we decided to improve <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1720002" target= " _blank">BBa_K1720002</a> (iGEM15_SCUT-China) and <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1456004" target= " _blank">BBa_K1456004</a> (iGEM14_ATOMS-Turkiye). So we sent in <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2295003" target= " _blank">BBa_K2295003</a>, to fulfill the needs further iGEM teams, dealing with hypoxia dependent gene expression, might have. </p> | ||
− | + | <p>Currently there is no BioBrick containing a minimal promoter in addition to the <i>HRE</i>, but only <i>HRE</i> itself (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1456004" target= " _blank">BBa_K1456004</a>) as well as a combination of <i>CMV-HRE-TATAbox</i> (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1720002" target= " _blank">BBa K1720002</a>). </p> | |
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Revision as of 19:53, 30 October 2017
BioBrick Improvement
At the beginning of our project, the iGEM part library was checked for parts that could be used for the CARTELTM AND gate. Since oxygen concentration plays a key role in the tumor microenvironment, we were especially interested in parts containing HREs (Hypoxia Response Elements). Though, since those BioBricks only covered the needs of our project partially, we decided to improve BBa_K1720002 (iGEM15_SCUT-China) and BBa_K1456004 (iGEM14_ATOMS-Turkiye). So we sent in BBa_K2295003, to fulfill the needs further iGEM teams, dealing with hypoxia dependent gene expression, might have.
Currently there is no BioBrick containing a minimal promoter in addition to the HRE, but only HRE itself (BBa_K1456004) as well as a combination of CMV-HRE-TATAbox (BBa K1720002).