Difference between revisions of "Team:MSU-Michigan/Current"
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<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3> | <h3>deltamtrB_GFPmtrB Induced V Uninduced</h3> | ||
| − | <figure><img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentmtrB170725.png" style="width: | + | <figure><img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentmtrB170725.png" style="width:50%"></figure> |
<p>Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.</p> | <p>Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.</p> | ||
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<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3> | <h3>deltamtrB_GFPmtrB Induced V Uninduced</h3> | ||
| − | <figure><img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentdeltamrtBGFPinduced.png" style="width= | + | <figure><img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentdeltamrtBGFPinduced.png" style="width=50%; max-width:800px"></figure> |
<p>The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.</p> | <p>The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.</p> | ||
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Revision as of 21:00, 30 October 2017
Current from Induction
deltamtrB_GFPmtrB Induced V Uninduced
Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.
deltamtrB_GFPmtrB Induced V Uninduced
The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.
Multiple Strains
Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.
Anaerobic Current
Anaerobic current showing how current induction can take the place of GFP induction as a marker for gene expression. IPTG induction occurs right at the small dip right around the 2 hour mark.
