Difference between revisions of "Team:RPI Troy NY/InterLab"

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<font face= "Times New Roman"> <font size= "3"><p>  Steps with an asterisk are not required in the iGEM interlab study, rather they are control steps
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<td style="width:80% align:center"><font face= "Times New Roman"> <font size= "3"><p>  Steps with an asterisk are not required in the iGEM interlab study, rather they are control steps
 
to preserve the DNA and ensure that if anything goes wrong, we have a stock to retransform
 
to preserve the DNA and ensure that if anything goes wrong, we have a stock to retransform
 
<p> We choose two colonies per plate so we can run biological duplicates. This can provide
 
<p> We choose two colonies per plate so we can run biological duplicates. This can provide
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to those details when the time comes
 
to those details when the time comes
 
<p> Form 1 can be filled out at any time, Form 2 requires spreadsheet data from plate reader, Form
 
<p> Form 1 can be filled out at any time, Form 2 requires spreadsheet data from plate reader, Form
3 is in regard to the culturing process</p>
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3 is in regard to the culturing process</p></td>
 
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Revision as of 00:15, 31 October 2017

Home Team Collaborations Project Results
Interlab Study Safety Human Practices Attributions

Interlab Study



We are very excited to announce we've transformed our DNA parts for the 2017 iGEM Interlab Study. Check out our Twitter page for weekly updates on our project!

We will be using DNA parts from Plate 7 in the distribution kit and are looking forward to collecting our first round of data.

♦Process Flow♦

 Steps with an asterisk are not required in the iGEM interlab study, rather they are control steps to preserve the DNA and ensure that if anything goes wrong, we have a stock to retransform

 We choose two colonies per plate so we can run biological duplicates. This can provide information on variability of compounds within a single organism (in this case, DH5a e. Coli)

 The iGEM plate reader protocol has a series of dilutions, so we will have to pay careful attention to those details when the time comes

 Form 1 can be filled out at any time, Form 2 requires spreadsheet data from plate reader, Form 3 is in regard to the culturing process