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<p>We will be using DNA parts from Plate 7 in the distribution kit and are looking forward to collecting our first round of data.</p> | <p>We will be using DNA parts from Plate 7 in the distribution kit and are looking forward to collecting our first round of data.</p> | ||
− | < | + | <center><h2><font face = "Palatino"><font size = "4"> ♦Process Flow♦</h2><center> |
<center><img src="https://static.igem.org/mediawiki/2017/d/d9/Rpi_interlab_process.png" width="800px" height="600px"><center> | <center><img src="https://static.igem.org/mediawiki/2017/d/d9/Rpi_interlab_process.png" width="800px" height="600px"><center> | ||
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<h2> <font face = "Palatino"><font size = "4">♦Protocols♦</h2> | <h2> <font face = "Palatino"><font size = "4">♦Protocols♦</h2> | ||
Revision as of 00:18, 31 October 2017
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Interlab Study
We are very excited to announce we've transformed our DNA parts for the 2017 iGEM Interlab Study. Check out our Twitter page Follow @RPIiGEM for weekly updates on our project!
We will be using DNA parts from Plate 7 in the distribution kit and are looking forward to collecting our first round of data.
♦Process Flow♦
![](https://static.igem.org/mediawiki/2017/d/d9/Rpi_interlab_process.png)
Steps with an asterisk are not required in the iGEM interlab study, rather they are control steps to preserve the DNA and ensure that if anything goes wrong, we have a stock to retransform We choose two colonies per plate so we can run biological duplicates. This can provide information on variability of compounds within a single organism (in this case, DH5a e. Coli) The iGEM plate reader protocol has a series of dilutions, so we will have to pay careful attention to those details when the time comes Form 1 can be filled out at any time, Form 2 requires spreadsheet data from plate reader, Form 3 is in regard to the culturing process |