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<h4>Introduction</h4> | <h4>Introduction</h4> | ||
− | <td><p>Used in conjunction with appropriate antibiotics to selectively plate</p> | + | <td><p>Used in conjunction with appropriate antibiotics to selectively plate</p></td> |
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+ | <h4>Materials</h4> | ||
+ | <td><p> LB Agar (NOT agarose), 35g/L | ||
+ | <p> appropriate antibiotic (ie, for 800mL LB Agar, add 800uL Amp, or 400Cm) | ||
+ | </p></td> | ||
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</td></tr></table> | </td></tr></table> |
Revision as of 00:22, 31 October 2017
Home | Team | Collaborations | Project | Results |
Interlab Study | Safety | Human Practices | Attributions |
Interlab Study
We are very excited to announce we've transformed our DNA parts for the 2017 iGEM Interlab Study. Check out our Twitter page Follow @RPIiGEM for weekly updates on our project!
We will be using DNA parts from Plate 7 in the distribution kit and are looking forward to collecting our first round of data.
♦Process Flow♦
![](https://static.igem.org/mediawiki/2017/d/d9/Rpi_interlab_process.png)
Steps with an asterisk are not required in the iGEM interlab study, rather they are control steps to preserve the DNA and ensure that if anything goes wrong, we have a stock to retransform We choose two colonies per plate so we can run biological duplicates. This can provide information on variability of compounds within a single organism (in this case, DH5a e. Coli) The iGEM plate reader protocol has a series of dilutions, so we will have to pay careful attention to those details when the time comes Form 1 can be filled out at any time, Form 2 requires spreadsheet data from plate reader, Form 3 is in regard to the culturing process |
♦Protocols♦
LB Agar Plates
Used in conjunction with appropriate antibiotics to selectively plate |
LB Agar (NOT agarose), 35g/L appropriate antibiotic (ie, for 800mL LB Agar, add 800uL Amp, or 400Cm) |