Difference between revisions of "Team:Berlin diagnostX/Experiments"

Line 196: Line 196:
 
         <div class="col-6">
 
         <div class="col-6">
 
             <h5 class="text-left igem_blue">Extension PCR </h5>
 
             <h5 class="text-left igem_blue">Extension PCR </h5>
             <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
+
             <p class="text-justify"> Toehold switches were constructed by two PCR-based steps, in which the recognition and hairpin region was attached to a LacZ reporter element. The construction was amplified from a circular DNA Template. </p>
 +
<p class="text-justify">Primer P1 and P2 are specifically designed for different Toehold switches.( Abb. 1) These include the Recognition-Structure, the characteristic Hairpin, Ribosome binding site and Linker. The Switches were constructed by ligating them to the lacZ reporter gene.</p>
 +
<p class="text-justify">A specific third step was used to purify the preceding amplifications and also attach a T7 Promotor region.</p>
 
         </div>
 
         </div>
 
     </div>
 
     </div>

Revision as of 00:37, 31 October 2017

Experiments