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Revision as of 01:35, 31 October 2017
We have formed online collaboration with NEFU since September. We informed each other about our progress and helped each other with experimental puzzles. Some of the problems we tackled together are listed as follows.
♝:We plasmid vector can't be cut.
♗:It may be because of the enzyme had losed potency :
Plasmid was contaminated with protein
The plasmid may be dissolved in a solution that inhibited the activity of the enzyme
♝:What should we do to link three parts efficiently?
♗:Use isocaudamer.
♝: What should we do when we we want to dye the sample by Comassie blue staining and WB.
♗:When you add the samlpe, you should pay attention to the amount of it. Measure the value of OD to get the concentration of the sample you need and then add the sample. When making up polyacrylamide agarose gel, wash and dry the holder. In that way, you can get the gel free of bubble.
After we obtained the plasmid from OUC-China, we transformed the four plasmids into the yeast strain EBY100 and sampled the yeast growth stabilizer. The fluorescence and Abs600 of the four strains were determined by a microplate reader.
We gave the result to the team,OUC. They are glad to see their results was repeated in our lab environment and was gotten an ideal result.
Address
Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046
nanjing_china@163.com
Social media
Twitter button:
iGEM Nanjing-China@nanjing_china
