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<h2 style="text-align:center">Overview</h2> | <h2 style="text-align:center">Overview</h2> | ||
<h4>The aim of the interlab this year was to explore-- | <h4>The aim of the interlab this year was to explore-- | ||
− | <br> "How close can the numbers be when fluorescence is measured all around the world?" | + | <br> "How close can the numbers be when fluorescence is measured all around the world?" |
<br> Participating in the interlab was a valuable experience, and it allowed us to make use of technology | <br> Participating in the interlab was a valuable experience, and it allowed us to make use of technology | ||
we had at our disposal that otherwise wasn’t relevant to our project. It added an extra dimension | we had at our disposal that otherwise wasn’t relevant to our project. It added an extra dimension | ||
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<br> | <br> | ||
<br> 1. OD600 reference point | <br> 1. OD600 reference point | ||
− | <div align="center"><img src="https://static.igem.org/mediawiki/2017/c/c0/Interlab-1.1.png" class="img-responsive" width=" | + | <div align="center"> |
+ | <img src="https://static.igem.org/mediawiki/2017/c/c0/Interlab-1.1.png" class="img-responsive" width="40%" height="40%"> | ||
+ | </div> | ||
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<br> | <br> | ||
− | <br> | + | <br> 2. Fluorescein standard curve |
− | + | <div class="col-md-12"> | |
− | + | <div class="col-md-6"> | |
− | + | <img src="https://static.igem.org/mediawiki/2017/9/91/Interlab-1.2.png" class="img-responsive"> | |
− | + | </div> | |
− | + | <div class="col-md-6"> | |
− | + | <img src="https://static.igem.org/mediawiki/2017/a/a0/Interlab-2.1.png" class="img-responsive"> | |
− | + | </div> | |
− | + | </div> | |
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</h4> | </h4> | ||
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+ | <br> | ||
+ | <br> | ||
+ | <div style="padding-top:30px"> | ||
+ | <h4> 3. Raw Plate Reader Measurements</h4> | ||
+ | </div> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/6d/Interlab_%281%29.jpg" class="img-responsive"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/dd/Interlab_%282%29.jpg" class="img-responsive"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br> | ||
+ | <br> | ||
+ | <div class="col-md-12" style="padding-top:20px"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/91/Interlab_%283%29.jpg" class="img-responsive"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f5/Interlab_%284%29.jpg" class="img-responsive"> | ||
+ | </div> | ||
+ | </div> | ||
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+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <div style="padding-top:600px"></div> | ||
+ | <h2 style="text-align:center" >Observation</h2> | ||
+ | <h4> | ||
+ | 1. It can be easily observed that Device1 and Device 4 didn’t grow after 6 hours. In fact, Device1 express high level of | ||
+ | GFP after being cultured all night long. | ||
+ | <div align="center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/ff/Interlab-gfp.jpg" class="img-responsive" width="40%" height="40%"> | ||
+ | </div> | ||
+ | |||
+ | <br> | ||
+ | <br> 2.In Fluorescein standard curve, it’s not linear when the concentration is high. But the Fluorescein | ||
+ | in Raw Plate Reader Measurements doesn’t reach the the non-linear region so the standard curve | ||
+ | is believable. | ||
+ | <br> | ||
+ | <br> 3. It was found there was great variation in readings when using different settings on the plate | ||
+ | reader. Given that iGEM HQ has kept the protocol consistent, it is suggested that the desired | ||
+ | plate reader settings are also specified to ensure consistency throughout the results. | ||
+ | <br> | ||
+ | <br> 4. The dilution forum provided by iGEM foundation is useful. After dilution, almost every OD600 | ||
+ | is around 0.04. | ||
+ | </h4> | ||
</div> | </div> |
Latest revision as of 03:19, 31 October 2017