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− | {{NPU-China}} | + | {{NPU-China/mmp}} |
− | <html> | + | <html lang="en"> |
+ | <head> | ||
+ | <!-- Bootstrap Core CSS --> | ||
+ | <link href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" rel="stylesheet"> | ||
− | < | + | <!-- Custom CSS --> |
− | < | + | <link href="https://2017.igem.org/Template:NPU-China/css?action=raw&ctype=text/css" rel="stylesheet"> |
− | + | ||
− | + | ||
− | + | ||
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− | < | + | <!-- Custom Fonts --> |
− | + | <link href="https://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet"> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | < | + | <!-- jQuery --> |
− | </ | + | <script src="https://cdn.bootcss.com/jquery/3.2.1/jquery.min.js"></script> |
+ | <!-- Bootstrap Core JavaScript --> | ||
+ | <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js"></script> | ||
+ | <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries --> | ||
+ | <!-- WARNING: Respond.js doesn't work if you view the page via file:// --> | ||
+ | <!--[if lt IE 9]> | ||
+ | <script src="https://oss.maxcdn.com/libs/html5shiv/3.7.0/html5shiv.js"></script> | ||
+ | <script src="https://oss.maxcdn.com/libs/respond.js/1.4.2/respond.min.js"></script> | ||
+ | <![endif]--> | ||
+ | <style> | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <!-- Navigation --> | ||
+ | <nav class="navbar navbar-inverse navbar-fixed-top" role="navigation"> | ||
+ | <div class="container"> | ||
+ | <div class="navbar-header"> | ||
+ | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1"> | ||
+ | <span class="sr-only">Toggle navigation</span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | </button> | ||
+ | <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/29/NPU-logo.png" style="max-width:50px;margin-top:-10px;"> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> | ||
+ | <ul class="nav navbar-nav navbar-right"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China">Home</a> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> | ||
+ | </li> | ||
+ | <li class="active"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
+ | |||
+ | <!-- Page Content --> | ||
+ | <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/a/a6/%E9%A2%98%E7%9B%AE%E5%B0%8F%E9%80%9A%E6%A0%8Finterlab.jpg"> | ||
+ | <div class="container" style="padding-top:70px"> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-12"> | ||
+ | <h2 style="text-align:center">Overview</h2> | ||
+ | <h4>The aim of the interlab this year was to explore-- | ||
+ | <br> "How close can the numbers be when fluorescence is measured all around the world?" | ||
+ | <br> Participating in the interlab was a valuable experience, and it allowed us to make use of technology | ||
+ | we had at our disposal that otherwise wasn’t relevant to our project. It added an extra dimension | ||
+ | to our lab work, and was a lot of fun! | ||
+ | <br> | ||
+ | <br> 1. OD600 reference point | ||
+ | <div align="center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/c/c0/Interlab-1.1.png" class="img-responsive" width="40%" height="40%"> | ||
+ | </div> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> 2. Fluorescein standard curve | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/91/Interlab-1.2.png" class="img-responsive"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/a/a0/Interlab-2.1.png" class="img-responsive"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </h4> | ||
+ | |||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <div style="padding-top:30px"> | ||
+ | <h4> 3. Raw Plate Reader Measurements</h4> | ||
+ | </div> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/6d/Interlab_%281%29.jpg" class="img-responsive"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/dd/Interlab_%282%29.jpg" class="img-responsive"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br> | ||
+ | <br> | ||
+ | <div class="col-md-12" style="padding-top:20px"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/91/Interlab_%283%29.jpg" class="img-responsive"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f5/Interlab_%284%29.jpg" class="img-responsive"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <div style="padding-top:600px"></div> | ||
+ | <h2 style="text-align:center" >Observation</h2> | ||
+ | <h4> | ||
+ | 1. It can be easily observed that Device1 and Device 4 didn’t grow after 6 hours. In fact, Device1 express high level of | ||
+ | GFP after being cultured all night long. | ||
+ | <div align="center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/ff/Interlab-gfp.jpg" class="img-responsive" width="40%" height="40%"> | ||
+ | </div> | ||
+ | |||
+ | <br> | ||
+ | <br> 2.In Fluorescein standard curve, it’s not linear when the concentration is high. But the Fluorescein | ||
+ | in Raw Plate Reader Measurements doesn’t reach the the non-linear region so the standard curve | ||
+ | is believable. | ||
+ | <br> | ||
+ | <br> 3. It was found there was great variation in readings when using different settings on the plate | ||
+ | reader. Given that iGEM HQ has kept the protocol consistent, it is suggested that the desired | ||
+ | plate reader settings are also specified to ensure consistency throughout the results. | ||
+ | <br> | ||
+ | <br> 4. The dilution forum provided by iGEM foundation is useful. After dilution, almost every OD600 | ||
+ | is around 0.04. | ||
+ | </h4> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <!-- Blog Post Row --> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png" class="img-responsive"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </body> | ||
</html> | </html> |
Latest revision as of 03:19, 31 October 2017