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+ | <center><img src="https://static.igem.org/mediawiki/2017/4/46/T--Macquarie_Australia--REGEL.png" width="500px" height="283px"> </center> | ||
+ | <center> <p align="justify" style="width:500px;word-wrap:break-word"> | ||
+ | <b><i>Figure 1.</i></b> Agarose gel (1%) electrophoresis. Lanes 1 and 10 contain a Neb 1kb marker. Lanes 2, and 6 were negative controls. Lane 4 shows no bands and indicates failed PCR of AMP in KOD. Lanes 7-9 show no bands and indicate unsuccessful PCR of respective antibiotics in Quick cells. Lanes 3 and 5 show successful band of CAM and AMP respectively at ~1600 bp. Lanes 11-14 show bands of ~3000 bp psbMZHWK (PS2) plasmid as expected cut with Eco-RI (E), XbaI (X), SpeI (S) and PstI (P). Lanes 15-18 show successful cut of PS2 plasmid at ~1000 bp and ~2000 bp, indicating all restriction enzymes are functional in cutsmart buffer. </center> | ||
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Revision as of 07:26, 31 October 2017
Glossary
- FNR - Ferredoxin NADP+ reductase.
- Fer- Ferredoxin and Ferredoxin NADP+ reductase (FNR).
- Hyd- hyd 1 coding for the enzymatic part of the Hydrogenase molecular machine.
- Fer/Hyd- Fer and Hyd as described above in a new biobrick construct.
- HydEF- coding two of the three maturation enzymes.
- HydEFG- hyd EF and hyd G, coding all three maturation enzymes in a new biobrick construct.
- Fer/Hyd/EFG, Omega Ω or HGPCC - plasmid with all the necessary genes coding the total Hydrogenase molecular machine. Ferredoxin, Ferredoxin NADP+ reductase, Hyd 1, Hyd EF and Hyd G all in a new biobrick construct. This plasmid gained the official bio-brick name – Hydrogen Gas Producing Gene Cluster (HGPGC).
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Figure 1. Agarose gel (1%) electrophoresis. Lanes 1 and 10 contain a Neb 1kb marker. Lanes 2, and 6 were negative controls. Lane 4 shows no bands and indicates failed PCR of AMP in KOD. Lanes 7-9 show no bands and indicate unsuccessful PCR of respective antibiotics in Quick cells. Lanes 3 and 5 show successful band of CAM and AMP respectively at ~1600 bp. Lanes 11-14 show bands of ~3000 bp psbMZHWK (PS2) plasmid as expected cut with Eco-RI (E), XbaI (X), SpeI (S) and PstI (P). Lanes 15-18 show successful cut of PS2 plasmid at ~1000 bp and ~2000 bp, indicating all restriction enzymes are functional in cutsmart buffer. |
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