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<h4><strong><I>D.The Bridging PCR protocols</I></strong> </h4> | <h4><strong><I>D.The Bridging PCR protocols</I></strong> </h4> | ||
+ | <p>Bridge PCR: take normal protocol with following revision: PCR 2 bridge DNA sections with upper/midup or midlow/lower primers from model, midup and midlow primer should be designed overlap with at least 22 bp, PCR result DNA sections can be used together as models for 2nd PCR with upper/lower primers. </p> | ||
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Revision as of 08:38, 31 October 2017