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<h3> <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/analysing">Analyzing</a> </h3> | <h3> <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Project/toolbox/analysing">Analyzing</a> </h3> | ||
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The structural analysis of a protein can be used to study distances between non-canonical amino acids (ncAAs) with Foerster Resonance Energy Transfer (FRET). This provides measuring distances between specific incorporated amino acids in the target protein. To incorporate the ncAAs we provide three different tRNA/aminoacyl-synthetases (<a target="_blank" href="http://parts.igem.org/Part:BBa_K2201201">BBa_K2201201</a>, <a target="_blank" href="http://parts.igem.org/Part:BBa_K2201202">BBa_K2201202</a>, <a target="_blank" href="http://parts.igem.org/Part:BBa_K2201203">BBa_K2201203</a>) which incorporate in respose to the amber or the less used leucine codon. To demonstrate this tool we also developed a prion detection assay. | The structural analysis of a protein can be used to study distances between non-canonical amino acids (ncAAs) with Foerster Resonance Energy Transfer (FRET). This provides measuring distances between specific incorporated amino acids in the target protein. To incorporate the ncAAs we provide three different tRNA/aminoacyl-synthetases (<a target="_blank" href="http://parts.igem.org/Part:BBa_K2201201">BBa_K2201201</a>, <a target="_blank" href="http://parts.igem.org/Part:BBa_K2201202">BBa_K2201202</a>, <a target="_blank" href="http://parts.igem.org/Part:BBa_K2201203">BBa_K2201203</a>) which incorporate in respose to the amber or the less used leucine codon. To demonstrate this tool we also developed a prion detection assay. | ||
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Revision as of 12:51, 31 October 2017
![](https://static.igem.org/mediawiki/2017/e/e9/T--Bielefeld-CeBiTec--title-img-toolbox.jpg)
Toolbox Overview
Short summary
Analyzing
The structural analysis of a protein can be used to study distances between non-canonical amino acids (ncAAs) with Foerster Resonance Energy Transfer (FRET). This provides measuring distances between specific incorporated amino acids in the target protein. To incorporate the ncAAs we provide three different tRNA/aminoacyl-synthetases (BBa_K2201201, BBa_K2201202, BBa_K2201203) which incorporate in respose to the amber or the less used leucine codon. To demonstrate this tool we also developed a prion detection assay.