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<div id="section1" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | <div id="section1" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Introduction</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Introduction</h3> | ||
− | <p style="padding:10px">It is significant for synthetic biology to develop a reliable and repeatable measurement, the same to all the other engineering disciplines. We HUST-China have volunteered to test some RBS devices (BCDs) that are intended to make gene expression more precise and reliable by | + | <p style="padding:10px">It is significant for synthetic biology to develop a reliable and repeatable measurement, the same to all the other engineering disciplines. We HUST-China have volunteered to test some RBS devices (BCDs) that are intended to make gene expression more precise and reliable by measuring the expression level of GFP, in order to help the iGEM community collect data about how reliable these devices will turn out to be in labs around the world. |
</p> | </p> | ||
</div> | </div> | ||
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<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Instrument</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Instrument</h3> | ||
<h4><strong> What instrument did you use during your measurements? </strong> </h4> | <h4><strong> What instrument did you use during your measurements? </strong> </h4> | ||
− | <p> | + | <p>Plate reader</p> |
<h4><strong> Please provide the brand and model of your instrument.</strong> </h4> | <h4><strong> Please provide the brand and model of your instrument.</strong> </h4> | ||
<p>Flexstation 3</p> | <p>Flexstation 3</p> | ||
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<li>②Add 100 μl LUDOX 100 % into wells A1, B1, C1, D1</li> | <li>②Add 100 μl LUDOX 100 % into wells A1, B1, C1, D1</li> | ||
<li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | <li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | ||
− | <li>④Measure absorbance 600 nm of all samples in all standard measurement | + | <li>④Measure absorbance 600 nm of all samples in all standard measurement models in instrument</li> |
<li>⑤Import data into "Abs600" blue cells in provided Excel calibration sheet</li> | <li>⑤Import data into "Abs600" blue cells in provided Excel calibration sheet</li> | ||
</ul> | </ul> | ||
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− | <h4><strong> B1. Protocol for | + | <h4><strong> B1. Protocol for Fluorescence in fluorescence standard curve</strong> </h4> |
<h5><strong>Did you use pathlength correction during measurement? </strong> </h5> | <h5><strong>Did you use pathlength correction during measurement? </strong> </h5> | ||
<p>Yes</p> | <p>Yes</p> | ||
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<li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | <li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | ||
<li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | <li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | ||
− | <li>⑭ | + | <li>⑭ Take care not to continue serial dilution into column 12.</li> |
<li>⑮ Repeat dilution series for rows B, C, D. | <li>⑮ Repeat dilution series for rows B, C, D. | ||
<li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | <li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. |
Revision as of 13:31, 31 October 2017