Difference between revisions of "Team:Uppsala/Notebook"

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<h3>Week: 07/06-16/06</h3>
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- A safety demonstration was done by the Lab technicians and Project managers which enabled us to know the fire escapes, first aid and emergency contacts. Lab space was assigned.<br>
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- Buffers and stock solutions were prepared. LB media and LB-agar plates with different antibiotics (Kanamycin, Chloramphenicol and Tetracycline) were prepared.<br>
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- <i>E. coli DH5α</i> competent cells were prepared. Ordered constructs for the three genes (CCD, ADH and UGT) were digested with available enzyme (E and S) and ligated to Linearized plasmid backbones from the iGEM Kit.
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<p>- Prepared competent cells were tested with the iGEM Competent Cells Test kit. No growth was observed. Possible causes: Faulty competent cells, improper digestion or transformation protocol.<br>
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- Made DH5α competent cells with another new protocol to be tested and used later.</p>
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Revision as of 14:01, 31 October 2017