Difference between revisions of "Team:Wageningen UR/Part Collection"
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<h1>Collection of Parts</h1> | <h1>Collection of Parts</h1> | ||
| − | <p>Here you can find our best collection of | + | <p>Here you can find our best collection of parts. We took five different fluorescent proteins (mVenus, sfGFP, mCerulean, mRFP and eYFP) and we split them to be used in Bimolecular Fluorescence Complementation (BiFC) analysis. All these parts have been <a href="https://2017.igem.org/Team:Wageningen_UR/Results/Fluorescent">characterized</a> and our <a href="https://2017.igem.org/Team:Wageningen_UR/Composite_Part">Best Part</a> is a practical application of split proteins, used in the <a href="https://2017.igem.org/Team:Wageningen_UR/Results/SpecificVisualization">visualization system</a> of Mantis. </p> |
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<tr> | <tr> | ||
<td><a href=http://parts.igem.org/Part:BBa_K2387045>BBa_K2387045</a></td> | <td><a href=http://parts.igem.org/Part:BBa_K2387045>BBa_K2387045</a></td> | ||
| − | <td>mVenus under Arabinose | + | <td>mVenus under Arabinose promoter</td> |
<td>José Manuel</td> | <td>José Manuel</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href=http://parts.igem.org/Part:BBa_K2387046>BBa_K2387046</a></td> | <td><a href=http://parts.igem.org/Part:BBa_K2387046>BBa_K2387046</a></td> | ||
| − | <td>Split mVenus under Arabinose | + | <td>Split mVenus under Arabinose promoter</td> |
<td>José Manuel</td> | <td>José Manuel</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href=http://parts.igem.org/Part:BBa_K2387047>BBa_K2387047</a></td> | <td><a href=http://parts.igem.org/Part:BBa_K2387047>BBa_K2387047</a></td> | ||
| − | <td>sfGFP under Arabinose | + | <td>sfGFP under Arabinose promoter</td> |
<td>José Manuel</td> | <td>José Manuel</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href=http://parts.igem.org/Part:BBa_K2387048>BBa_K2387048</a></td> | <td><a href=http://parts.igem.org/Part:BBa_K2387048>BBa_K2387048</a></td> | ||
| − | <td>Split sfGFP under Arabinose | + | <td>Split sfGFP under Arabinose promoter</td> |
<td>José Manuel</td> | <td>José Manuel</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href=http://parts.igem.org/Part:BBa_K2387052>BBa_K2387052</a></td> | <td><a href=http://parts.igem.org/Part:BBa_K2387052>BBa_K2387052</a></td> | ||
| − | <td>mCerulean under Arabinose | + | <td>mCerulean under Arabinose promoter</td> |
<td>José Manuel</td> | <td>José Manuel</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href=http://parts.igem.org/Part:BBa_K2387053>BBa_K2387053</a></td> | <td><a href=http://parts.igem.org/Part:BBa_K2387053>BBa_K2387053</a></td> | ||
| − | <td>Split mCerulean under Arabinose | + | <td>Split mCerulean under Arabinose promoter</td> |
<td>José Manuel</td> | <td>José Manuel</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href=http://parts.igem.org/Part:BBa_K2387054>BBa_K2387054</a></td> | <td><a href=http://parts.igem.org/Part:BBa_K2387054>BBa_K2387054</a></td> | ||
| − | <td>mRFP under Arabinose | + | <td>mRFP under Arabinose promoter</td> |
<td>José Manuel</td> | <td>José Manuel</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href=http://parts.igem.org/Part:BBa_K2387055>BBa_K2387055</a></td> | <td><a href=http://parts.igem.org/Part:BBa_K2387055>BBa_K2387055</a></td> | ||
| − | <td>Split mRFP under Arabinose | + | <td>Split mRFP under Arabinose promoter</td> |
<td>José Manuel</td> | <td>José Manuel</td> | ||
</tr> | </tr> | ||
Revision as of 14:11, 31 October 2017
Collection of Parts
Here you can find our best collection of parts. We took five different fluorescent proteins (mVenus, sfGFP, mCerulean, mRFP and eYFP) and we split them to be used in Bimolecular Fluorescence Complementation (BiFC) analysis. All these parts have been characterized and our Best Part is a practical application of split proteins, used in the visualization system of Mantis.
| BioBrick Code | Part Name | Designer |
|---|---|---|
| BBa_K2387045 | mVenus under Arabinose promoter | José Manuel |
| BBa_K2387046 | Split mVenus under Arabinose promoter | José Manuel |
| BBa_K2387047 | sfGFP under Arabinose promoter | José Manuel |
| BBa_K2387048 | Split sfGFP under Arabinose promoter | José Manuel |
| BBa_K2387052 | mCerulean under Arabinose promoter | José Manuel |
| BBa_K2387053 | Split mCerulean under Arabinose promoter | José Manuel |
| BBa_K2387054 | mRFP under Arabinose promoter | José Manuel |
| BBa_K2387055 | Split mRFP under Arabinose promoter | José Manuel |
| BBa_K2387003 | eYFP + araC/pBAD promoter | Bart |
| BBa_K2387065 | eYFP[1-154]-nLeucine Zipper and eYFP[155-238]-cLeucine Zipper fusions controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387032 | CpxR-eYFPn and CpxR-eYPFc fusions controlled by inducible araC/pBAD promoter | Bart |
