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<h4><strong>①Amplify</strong> </h4> | <h4><strong>①Amplify</strong> </h4> | ||
<p> | <p> | ||
− | <I><I><I><I><I><I><I>pmrA-B-Fe</I></I></I></I></I></I></I>、 <I> <I> pmrC | + | <I><I><I><I><I><I><I>pmrA-B-Fe</I></I></I></I></I></I></I>、 <I> <I> <I> pmrC-GFP</I></I></I> gene through the pcr protocol |
</p> | </p> | ||
<h4><strong>②Plasmid Construction</strong> </h4> | <h4><strong>②Plasmid Construction</strong> </h4> | ||
<p> | <p> | ||
− | Coding sequences pmrA | + | Coding sequences <I> pmrA-pmrB</I> and pmrC-GFP were cloned into the expression vector pBAD30 by restriction enzyme digestion and DNA ligation. |
</p> | </p> | ||
<div class="experiment_circuit col-md-4 col-xs-4 col-md-offset-4 col-xs-offset-4"> | <div class="experiment_circuit col-md-4 col-xs-4 col-md-offset-4 col-xs-offset-4"> |
Revision as of 14:17, 31 October 2017