Line 480: | Line 480: | ||
<h4><strong>②Plasmid Construction</strong> </h4> | <h4><strong>②Plasmid Construction</strong> </h4> | ||
<p> | <p> | ||
− | Coding sequences <I> pmrA-pmrB</I> and <I> <I> <I> pmrC- | + | Coding sequences <I> pmrA-pmrB</I> and <I> <I> <I> <I> pmrC-gfp </I></I></I></I>were cloned into the expression vector pBAD30 by restriction enzyme digestion and DNA ligation. |
</p> | </p> | ||
<div class="experiment_circuit col-md-4 col-xs-4 col-md-offset-4 col-xs-offset-4"> | <div class="experiment_circuit col-md-4 col-xs-4 col-md-offset-4 col-xs-offset-4"> | ||
Line 494: | Line 494: | ||
</p> | </p> | ||
<p> | <p> | ||
− | We use the original pmrA-pmrB / Fe- <I> <I> <I> pmrC- | + | We use the original pmrA-pmrB / Fe- <I> <I> <I> <I> pmrC-gfp </I></I></I></I>circuit to test whether our |
pmrABC system works in our plasmid, then the expression and efficacy | pmrABC system works in our plasmid, then the expression and efficacy | ||
of LBT1-12 were tested . | of LBT1-12 were tested . | ||
Line 517: | Line 517: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>pmrA-pmrB/LBT1- <I> <I> <I> pmrC- | + | <td>pmrA-pmrB/LBT1- <I> <I> <I> <I> pmrC-gfp </I></I></I></I></td> |
<td>pBAD30</td> | <td>pBAD30</td> | ||
<td> Detecting effectiveness of protein LBT1</td> | <td> Detecting effectiveness of protein LBT1</td> | ||
Line 542: | Line 542: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>pmrA-pmrB/LBT6- <I> <I> <I> pmrC- | + | <td>pmrA-pmrB/LBT6- <I> <I> <I> <I> pmrC-gfp </I></I></I></I></td> |
<td>pBAD30</td> | <td>pBAD30</td> | ||
<td> Detecting effectiveness of protein LBT6</td> | <td> Detecting effectiveness of protein LBT6</td> | ||
Line 572: | Line 572: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>pmrA-pmrB/LBT12- <I> <I> <I> pmrC- | + | <td>pmrA-pmrB/LBT12- <I> <I> <I> <I> pmrC-gfp </I></I></I></I></td> |
<td> pBAD30 </td> | <td> pBAD30 </td> | ||
<td>Detecting effectiveness of protein LBT12</td> | <td>Detecting effectiveness of protein LBT12</td> |
Revision as of 14:26, 31 October 2017