Line 476: | Line 476: | ||
<h4><strong>①Amplify</strong> </h4> | <h4><strong>①Amplify</strong> </h4> | ||
<p> | <p> | ||
− | <I><I><I><I><I><I><I>pmrA-B-Fe</I></I></I></I></I></I></I>、<I><I><I><I>pmrC-gfp </I></I></I></I> gene through the pcr protocol | + | <I><I><I><I><I><I><I>pmrA-B-Fe</I></I></I></I></I></I></I>、 <I> <I> <I> <I> pmrC-gfp </I></I></I></I> gene through the pcr protocol |
</p> | </p> | ||
<h4><strong>②Plasmid Construction</strong> </h4> | <h4><strong>②Plasmid Construction</strong> </h4> | ||
Line 494: | Line 494: | ||
</p> | </p> | ||
<p> | <p> | ||
− | We use the original <I>pmrA-pmrB-Fe-pmrC-gfp </I>circuit to test whether our | + | We use the original <I> <I> pmrA-pmrB-Fe </I> </I>-<I> <I> <I> <I> pmrC-gfp </I></I></I></I>circuit to test whether our |
pmrABC system works in our plasmid, then the expression and efficacy | pmrABC system works in our plasmid, then the expression and efficacy | ||
of LBT1-12 were tested . | of LBT1-12 were tested . | ||
Line 611: | Line 611: | ||
<div id="section4" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | <div id="section4" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Detection of sensing part </h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Detection of sensing part </h3> | ||
− | <p>We use our medium containing a small amount of rare earth ions to culture our E. coli. When our engineered E. coli | + | <p>We use our medium containing a small amount of rare earth ions to culture our E. coli. When our engineered E. coli sense the existence of rare earth ions, the pmrC promoter will activate the transcription of the GFP gene. Through the detection of GFP expression, we can know the efficacy of the sensing part. </p> |
− | + | ||
</div> | </div> | ||
Revision as of 15:35, 31 October 2017