Difference between revisions of "Team:UNOTT/Results"

 
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<head>
 
<head>
 
   <meta charset="UTF-8">
 
   <meta charset="UTF-8">
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   <title>Expandable Boxes With CSS Transitions</title>
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   <script src="https://s.codepen.io/assets/libs/modernizr.js" type="text/javascript"></script>
 
   <script src="https://s.codepen.io/assets/libs/modernizr.js" type="text/javascript"></script>
  
  
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  <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/normalize/5.0.0/normalize.min.css">
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<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/normalize/5.0.0/normalize.min.css">
 
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/angular.js/1.5.5/angular.min.js">
 
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/angular.js/1.5.5/angular.min.js">
 
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.6/css/bootstrap.min.csss">
 
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.6/css/bootstrap.min.csss">
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<link href="https://fonts.googleapis.com/css?family=Montserrat" rel="stylesheet">
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<link href="https://fonts.googleapis.com/css?family=Karla" rel="stylesheet">
  
 
<style>
 
<style>
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body {
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    font-family: 'Karla', sans-serif;
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p {
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margin-top:10px;
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h2 span.spacer {
 
h2 span.spacer {
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   left: 0;  
 
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   width: 100%;
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  font-family: 'Karla', sans-serif;
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}
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h4 {
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  font-family: 'Karla', sans-serif;
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  color:#4b524a;
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   margin: auto;
 
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     width: 60%;
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    border: 3px solid #73AD21;
 
 
     padding: 10px;
 
     padding: 10px;
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    font-family: 'Karla', sans-serif;
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    background-color: #ffffff;
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}
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.wrapper {
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  width: 100%;
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  margin: 0 auto;
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  padding: 3em 0 0;
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}
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.dropdown1 {
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  float: left;
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  width: 100%;
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  position: relative;
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  padding-bottom: 2em;
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}
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.dropdown1 input[type=checkbox] {
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  position: absolute;
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  top: 30px;
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  left: 0;
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  height: 100%;
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  opacity: 0;
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  cursor: pointer;
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.dropdown1 label {
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  cursor: pointer;
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  display: inline-block;
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  padding-left: 1.75rem;
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  font-size: 5.0rem;
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  line-height: 2rem;
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  font-weight: 600;
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  color: #333;
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  font-family: Karla, sans-serif;
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}
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.dropdown1 label:before {
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  content: "";
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  position: absolute;
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  top: 6px;
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  left: 0;
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  border-left: 8px solid #F5634A;
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  border-top: 8px solid transparent;
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  border-bottom: 8px solid transparent;
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  margin-left: 4px;
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}
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.dropdown1 input[type=checkbox]:checked ~ label:before {
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  border-left: 8px solid transparent;
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  border-top: 8px solid #F5634A;
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  border-right: 8px solid transparent;
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  margin: 8px 0 0;
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}
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.dropdown1 .text {
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  max-height: 0;
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  -webkit-transition: max-height 1s;
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  -moz-transition: max-height 1s;
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  -ms-transition: max-height 1s;
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  -o-transition: max-height 1s;
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  transition: max-height 1s; 
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  overflow: hidden;
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  font-family: Karla, serif;
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  font-size: 1.2rem;
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  line-height: 1.6rem;
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  letter-spacing: 0.05em;
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  color: #333;
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  padding-left: 1.75rem;
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}
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.dropdown1 input[type=checkbox]:checked ~ .text {
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  max-height: 1000px;
 
}
 
}
 
</style>
 
</style>
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<body>
 
<body>
  
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                  <link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet">
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<p><br>
 
<p><br>
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<h4>OBJECTIVE: CREATE pgRNA</h4>
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   <h4>OBJECTIVE: CREATE pREPORTER</h4>
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   <h4>OBJECTIVE: PROMOTER LIBRARY</h4>
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  <h4>OBJECTIVE: RANDOM LIGATIONS</h4>
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      <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">OBJECTIVE: FREEZE DRYING</h4>
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  <h4>OBJECTIVE: CRISPRi & gRNA EFFICIENCY</h4>
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<p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid,
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<div class="wrapper">
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  <div class="dropdown1">
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  <input type="checkbox" id="question-1">
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  <label for="question-1"> STEP 1: Create guideRNA Plasmid </label>
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    <div class="text">
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      <p>Text</p>
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    </div>
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  </div>
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<br> 
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<div class="dropdown1">
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  <input type="checkbox" id="question-2">
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  <label for="question-2"> STEP 2: Create Reporter Plasmid </label>
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    <div class="text">
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      <p>Text</p>
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    </div>
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  </div>
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<br> 
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<div class="dropdown1">
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  <input type="checkbox" id="question-3">
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  <label for="question-3">  STEP 3: Create Promoter Library </label>
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    <div class="text">
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      <p>Text</p>
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    </div>
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  </div>
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<br>
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<div class="dropdown1">
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  <input type="checkbox" id="question-4">
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  <label for="question-4">STEP 4: Random Ligations      </label>
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    <div class="text">
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      <p>Text</p>
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    </div>
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  </div>
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<br>
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<div class="dropdown1">
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  <input type="checkbox" id="question-5">
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  <label for="question-5">STEP 5: Freeze drying & Revival    </label>
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    <div class="text">
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      <p>Here are the results of our freeze-dried cells revival experiments. With these experiments, we want to show that different storage do not have an effect on the revival of the cells within our key.</p>
 +
    </div>
 +
  </div>
 +
 
 +
<br>
 +
 
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<div class="dropdown1">
 +
  <input type="checkbox" id="question-6">
 +
  <label for="question-6"> STEP 6: CRISPRi & guideRNA efficiency    </label>
 +
    <div class="text">
 +
      <p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid,
 
  or with a non-targeting gRNA plasmid.</p>
 
  or with a non-targeting gRNA plasmid.</p>
 
<img src="https://static.igem.org/mediawiki/2017/6/6a/T--UNOTT--gRNAresultspic.jpeg">
 
<img src="https://static.igem.org/mediawiki/2017/6/6a/T--UNOTT--gRNAresultspic.jpeg">
 +
<img src="https://static.igem.org/mediawiki/2017/e/e1/T--UNOTT--photosofgRNAresults.jpeg">
  
 +
    </div>
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  </div>
  
  
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</body>
 
</body>

Latest revision as of 16:11, 31 October 2017





RESULTS:

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Here are the results of our freeze-dried cells revival experiments. With these experiments, we want to show that different storage do not have an effect on the revival of the cells within our key.


This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.