Difference between revisions of "Team:UNOTT/Results"

 
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  <h4>STEP 1: Create guideRNA Plasmid</h4>
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   <h4>STEP 2: Create Reporter Plasmid </h4>
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   <h4>STEP 3 : Create Promoter Library</h4>
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   <h4>STEP 4 : Random Ligations      </h4>
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  <h4>STEP 5: Freeze drying & Revivial</h4>
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   <h4>STEP 6: CRISPRi & guideRNA efficiency</h4>
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<p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid,
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  <label for="question-1"> STEP 1: Create guideRNA Plasmid </label>
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      <p>Text</p>
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  <input type="checkbox" id="question-2">
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  <label for="question-2"> STEP 2: Create Reporter Plasmid </label>
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      <p>Text</p>
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  <input type="checkbox" id="question-3">
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      <p>Text</p>
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<div class="dropdown1">
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  <input type="checkbox" id="question-4">
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  <label for="question-4">STEP 4: Random Ligations      </label>
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      <p>Text</p>
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  <label for="question-5">STEP 5: Freeze drying & Revival    </label>
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    <div class="text">
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      <p>Here are the results of our freeze-dried cells revival experiments. With these experiments, we want to show that different storage do not have an effect on the revival of the cells within our key.</p>
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<br>
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<div class="dropdown1">
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  <input type="checkbox" id="question-6">
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  <label for="question-6"> STEP 6: CRISPRi & guideRNA efficiency    </label>
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    <div class="text">
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      <p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid,
 
  or with a non-targeting gRNA plasmid.</p>
 
  or with a non-targeting gRNA plasmid.</p>
 
<img src="https://static.igem.org/mediawiki/2017/6/6a/T--UNOTT--gRNAresultspic.jpeg">
 
<img src="https://static.igem.org/mediawiki/2017/6/6a/T--UNOTT--gRNAresultspic.jpeg">
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<img src="https://static.igem.org/mediawiki/2017/e/e1/T--UNOTT--photosofgRNAresults.jpeg">
  
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Latest revision as of 16:11, 31 October 2017





RESULTS:

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Here are the results of our freeze-dried cells revival experiments. With these experiments, we want to show that different storage do not have an effect on the revival of the cells within our key.


This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.