Line 512: | Line 512: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/Fe-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/Fe-PmrC-GFP</I></td> |
<td>pBAD30</td> | <td>pBAD30</td> | ||
<td>Test whether our PmrABC system works in our plasmid</td> | <td>Test whether our PmrABC system works in our plasmid</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT1-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT1-PmrC-GFP</I></td> |
<td>pBAD30</td> | <td>pBAD30</td> | ||
<td> Detecting effectiveness of protein LBT1</td> | <td> Detecting effectiveness of protein LBT1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT2-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT2-PmrC-GFP</I></td> |
<td>pBAD30 </td> | <td>pBAD30 </td> | ||
<td> Detecting effectiveness of protein LBT2</td> | <td> Detecting effectiveness of protein LBT2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT3-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT3-PmrC-GFP</I></td> |
<td>pBAD30 </td> | <td>pBAD30 </td> | ||
<td>Detecting effectiveness of protein LBT3</td> | <td>Detecting effectiveness of protein LBT3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT4-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT4-PmrC-GFP</I></td> |
<td> pBAD30 </td> | <td> pBAD30 </td> | ||
<td>Detecting effectiveness of protein LBT4</td> | <td>Detecting effectiveness of protein LBT4</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT5-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT5-PmrC-GFP</I></td> |
<td>pBAD30</td> | <td>pBAD30</td> | ||
<td>Detecting effectiveness of protein LBT5</td> | <td>Detecting effectiveness of protein LBT5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT6-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT6-PmrC-GFP</I></td> |
<td>pBAD30</td> | <td>pBAD30</td> | ||
<td> Detecting effectiveness of protein LBT6</td> | <td> Detecting effectiveness of protein LBT6</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT7-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT7-PmrC-GFP</I></td> |
<td>pBAD30 </td> | <td>pBAD30 </td> | ||
<td> Detecting effectiveness of protein LBT7</td> | <td> Detecting effectiveness of protein LBT7</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT8-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT8-PmrC-GFP</I></td> |
<td>pBAD30 </td> | <td>pBAD30 </td> | ||
<td>Detecting effectiveness of protein LBT8</td> | <td>Detecting effectiveness of protein LBT8</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT9-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT9-PmrC-GFP</I></td> |
<td> pBAD30 </td> | <td> pBAD30 </td> | ||
<td>Detecting effectiveness of protein LBT9</td> | <td>Detecting effectiveness of protein LBT9</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT10-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT10-PmrC-GFP</I></td> |
<td>pBAD30 </td> | <td>pBAD30 </td> | ||
<td> Detecting effectiveness of protein LBT10</td> | <td> Detecting effectiveness of protein LBT10</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT11-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT11-PmrC-GFP</I></td> |
<td>pBAD30 </td> | <td>pBAD30 </td> | ||
<td>Detecting effectiveness of protein LBT11</td> | <td>Detecting effectiveness of protein LBT11</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>PmrA-PmrB/LBT12-PmrC-GFP</td> | + | <td><I>PmrA-PmrB/LBT12-PmrC-GFP</I></td> |
<td> pBAD30 </td> | <td> pBAD30 </td> | ||
<td>Detecting effectiveness of protein LBT12</td> | <td>Detecting effectiveness of protein LBT12</td> | ||
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<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Construction of the whole circuit</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Construction of the whole circuit</h3> | ||
<p> | <p> | ||
− | <I> PmrA-PmrB-Fe-PmrC-GFP</I> All of our part should be built in one plasmid. Therefore, we use endonuclease and ligase to replace the GFP by oprF-LBT and oprF- | + | <I> PmrA-PmrB-Fe-PmrC-GFP</I> All of our part should be built in one plasmid. Therefore, we use endonuclease and ligase to replace the <I>GFP</I> by <I>oprF-LBT</I> and <I>oprF-Sitag</I>. |
</p> | </p> | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/5/58/2017_HUST_China_Experiment_image4.png" alt="experiment_circuit" alt="demo1" class="col-xs-4 col-xs-offset-4" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/5/58/2017_HUST_China_Experiment_image4.png" alt="experiment_circuit" alt="demo1" class="col-xs-4 col-xs-offset-4" style="padding: 10px 0px;"> | ||
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<div id="section4" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | <div id="section4" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Detection of sensing part </h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Detection of sensing part </h3> | ||
− | <p>We use our medium containing a small amount of rare earth ions to culture our E. coli. When our engineered E. coli sense the existence of rare earth ions, the PmrC promoter will activate the transcription of the GFP gene. Through the detection of GFP expression, we can know the efficacy of the sensing part. </p> | + | <p>We use our medium containing a small amount of rare earth ions to culture our E. coli. When our engineered E. coli sense the existence of rare earth ions, the PmrC promoter will activate the transcription of the <I>GFP</I> gene. Through the detection of <I>GFP</I> expression, we can know the efficacy of the sensing part. </p> |
</div> | </div> | ||
Revision as of 18:38, 31 October 2017