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<li><a href="#section5">Calibration Protocol</a></li> | <li><a href="#section5">Calibration Protocol</a></li> | ||
<li><a href="#section6">Cell Culture</a></li> | <li><a href="#section6">Cell Culture</a></li> | ||
− | <li><a href="#section7">Interlab | + | <li><a href="#section7">Interlab Results</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<tr> | <tr> | ||
<td>Haibo Huang </td> | <td>Haibo Huang </td> | ||
− | <td> | + | <td>2278190212@qq.com</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<tr> | <tr> | ||
<td>Shaofeng Liao</td> | <td>Shaofeng Liao</td> | ||
− | <td> | + | <td>1607278252@qq.com</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | ||
<h4><strong> What chassis did you use?</strong> </h4> | <h4><strong> What chassis did you use?</strong> </h4> | ||
− | <p>Escherichia coli | + | <p>Escherichia coli DH5α</p> |
<h4><strong> What Biosafety Level is your chassis? </strong> </h4> | <h4><strong> What Biosafety Level is your chassis? </strong> </h4> | ||
<p>BSL1</p> | <p>BSL1</p> | ||
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<li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | <li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | ||
<li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> | <li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
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<li>③ Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li> | <li>③ Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li> | ||
<li>④ *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li> | <li>④ *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
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<li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | <li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | ||
<li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | <li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | ||
− | <li>⑭ | + | <li>⑭ Take care not to continue serial dilution into column 12.</li> |
<li>⑮ Repeat dilution series for rows B, C, D. | <li>⑮ Repeat dilution series for rows B, C, D. | ||
<li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | <li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | ||
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<ul class="yuandian"> | <ul class="yuandian"> | ||
<li>Measure OD and fluorescence of all samples</li> | <li>Measure OD and fluorescence of all samples</li> | ||
− | |||
</ul> | </ul> | ||
<h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> | <h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> |
Revision as of 20:17, 31 October 2017