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| | <div class="col-4 text-center"><a href="#" type="button" class="btn btn-primary igem_but btn-lg" id="01">Project Management</a></div> | | <div class="col-4 text-center"><a href="#" type="button" class="btn btn-primary igem_but btn-lg" id="01">Project Management</a></div> |
| | <div class="col-4 text-center"><a href="#" type="button" class="btn btn-primary igem_but btn-lg" id="02">Experiments – Overview</a></div> | | <div class="col-4 text-center"><a href="#" type="button" class="btn btn-primary igem_but btn-lg" id="02">Experiments – Overview</a></div> |
| − | <div class="col-4 text- left"><a href="#" type="button" class="btn btn-primary igem_but btn-lg" id="03">Protocols</a></div> | + | <div class="col-4 text- center"><a href="#" type="button" class="btn btn-primary igem_but btn-lg px-5" id="03">Protocols</a></div> |
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| | </div> | | </div> |
| | </div> | | </div> |
| − | <div class="row mt-3">
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| − | <div class="col-6">
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| − | <h5 class="text-left igem_blue">The Tapeworm <em>Taenia solium</em> </h5>
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| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <div class="col-6">
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| − | <h5 class="text-left igem_blue">The Tapeworm <em>Taenia solium</em> </h5>
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| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <h3 class="text-center igem_blue mt-4"><strong>The Idea</strong></h3>
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| − | <h5 class="text-left igem_blue pb-1">The Tapeworm <em>Taenia solium</em> </h5>
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| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <div class="col-6">
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| − | <p class="text-justify">The test will be based on a newly developed method called toehold switch sensors. This method has already been successfully applied to the diagnosis of the Zika virus [3]. We are therefore convinced that toehold switch sensors will also be useful in detecting T. solium.
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| − | In order to develop our test, we first need to achieve two things:
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| − | The test will be based on a newly developed method called toehold switch sensors. This method has already been successfully applied to the diagnosis of the Zika virus [3]. We are therefore convinced that toehold switch sensors will also be useful in detecting T. solium.
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| − | In order to develop our test, we first need to achieve two things:
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| − | <p class="summarybox p-4"><strong>Summary</strong>: Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum.</p>
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| − | <h3 class="text-center igem_blue mt-4"><strong>The Experiments</strong></h3>
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| − | <h5 class="text-left igem_blue pb-1">Production of Toehold Switch Sensors </h5>
| |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | </div>
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| − | <div class="col-6">
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| − | <h5 class="text-left igem_blue pb-1">Toehold Switch Sensor Test</h5>
| |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | </div>
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| − | <div class="row large_image_element mt-4">
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| − | <div class="col-12">
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| − | <img src="./images/toeholdswitch_easy.png" class="img-fluid">
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| − | <h3 class="text-center igem_blue mt-4"><strong>Application</strong></h3>
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| − | <h5 class="igem_blue pb-1">Überschrift</h5>
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| − | <p class="text-justify">The synthetic biology experiments described here are particularly important for participation in the iGEM competition. Simultaneously, a second team will work on the recovery of T. solium-RNA from patient stool samples in order to make our test applicable for medical purposes. We are currently forming the necessary partnerships for this goal: A working group in Nairobi has already committed to providing us with relevant material. However, these experiments will be conducted in a properly equipped laboratory, independent from the previously described experiments.</p>
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| − | </div>
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| − | </div>
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| − | </div>
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| | </div> | | </div> |
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| − | <!-- Experiment Overview --> | + | <!-- Experiment Overview --><div id="inhaltsverzeichnis"> |
| − | <div id="experi" style="display: none;"><div id="inhaltsverzeichnis"> | + | |
| | <div class="row"> | | <div class="row"> |
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| | <li>Gel Electrophoresis</li> | | <li>Gel Electrophoresis</li> |
| | <li>DNA Clean-Up</li> | | <li>DNA Clean-Up</li> |
| − | </ol></li>
| + | </ol></li></ul> |
| | + | </div> |
| | + | <div class="col-6"> |
| | + | <ul> |
| | <li><a class="igem_blue" href="#switch_testing">Toehold Switch Sensor Test – Testing Pipeline</a> | | <li><a class="igem_blue" href="#switch_testing">Toehold Switch Sensor Test – Testing Pipeline</a> |
| | <ol> | | <ol> |
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| | <li>2. Preparation of sensors for iGEM Submission (Mutagenesis & Cloning)</li> | | <li>2. Preparation of sensors for iGEM Submission (Mutagenesis & Cloning)</li> |
| | </ol></li> | | </ol></li> |
| − | <p class="text-center"><strong>Please find detailed protocols under the "Protocol" Section</strong></p> | + | <p class="text-center mt-4"><strong>Please find detailed protocols under the "Protocol" Section</strong></p> |
| − | </ul> | + | </ul></div> |
| − | </div>
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| − | <div class="col-6"></div>
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| | </div> | | </div> |
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| − | <!-- Schema 1 -->
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| − | <h3 class="text-center igem_blue mt-4"><strong>The bioinformatics behind our switch design</strong></h3>
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| − | </div>
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| − | <h5 class="text-left igem_blue pb-1">In silico design </h5>
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| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <!-- Schema 1 -->
| + | </div> |
| − | <div class="row">
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| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue mt-4"><strong>The switch synthesis</strong></h3>
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| − | </div>
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| − | <img class="img-fluid" src="https://placekitten.com/550/400">
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| − | <h5 class="text-left igem_blue">Extension PCR </h5>
| + | |
| − | <p class="text-justify"> Toehold switches were constructed by two PCR-based steps, in which the recognition and hairpin region was attached to a LacZ reporter element. The construction was amplified from a circular DNA Template. </p>
| + | |
| − | <p class="text-justify">Primer P1 and P2 are specifically designed for different Toehold switches.( Abb. 1) These include the Recognition-Structure, the characteristic Hairpin, Ribosome binding site and Linker. The Switches were constructed by ligating them to the lacZ reporter gene.</p>
| + | |
| − | <p class="text-justify">A specific third step was used to purify the preceding amplifications and also attach a T7 Promotor region.</p>
| + | |
| − | </div>
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| − | </div>
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| − | <div class="row mt-3">
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| − | <div class="col-6">
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| − | <h5 class="text-left igem_blue pb-1">Nested PCR</h5>
| + | |
| − | <p class="text-justify"> Nested PCR is a method for speeding up the process of switch generation by combining the two steps of our extension-PCR in one cycling step. In order to extent the whole switch length without interruption, purification of P1-product and second PCR Set-Up. After consumption of P1-forward primer the already added P2 Primer takes over the reaction. </p>
| + | |
| − | <p class="text-justify"> Our experiments showed the suitability of nested PCR for the creation of diagnost-x toehold switches even though different to the situation described by Schuelke (1) our forward primers have equal overlaps and therefore are not suitable for modulating the annealing temperature in order to start the primers separately. Reactions with different relation in the amounts of P1 and P2 forward primer proved the relation of P1/P2=1/4 as the most reliable in sequence stability. </p>
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| − | <img class="img-fluid" src="https://placekitten.com/550/400">
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| − | <h5 class="text-left igem_blue">Colony PCR </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <div class="row mt-3">
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| − | <h5 class="text-left igem_blue pb-1">DNA Clean Up (via column & gel extraction) </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <div class="col-6">
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| − | <h5 class="text-left igem_blue">Gel electrophoresis </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <h3 class="text-center igem_blue mt-4"><strong>The Toehold Switch Sensor Test</strong></h3>
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| − | <h5 class="text-left igem_blue pb-1"> Standard screening in liquid phase </h5>
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| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <h5 class="text-left igem_blue"><strong> CFE on membrane trials (BSA vs Whatman membrane) </strong></h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <h3 class="text-center igem_blue mt-4"><strong>Taenia solium model</strong></h3>
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| − | <h5 class="text-left igem_blue pb-1"> RNA Library </h5>
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| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <img class="img-fluid" src="https://placekitten.com/550/400">
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| − | <!-- Schema 1 -->
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| − | <div class="row">
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| − | <div class="col-12">
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| − | <h3 class="text-center igem_blue mt-4"><strong>Optimizing of our screening pipeline</strong></h3>
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| − | <h5 class="text-left igem_blue pb-1"> Primer efficiency analysis & PCR characterization (P2 vs P3) </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
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| − | <div class="col-6">
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| − | <h5 class="text-left igem_blue"><strong>Optimizing CFE</strong> </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
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| − | </div>
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| − |
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| − | <!-- Seperator -->
| + | |
| − |
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| − | <div class="row"><div class="col"><hr class="mt-5 mb-5 igem_seperator mx-auto"></div></div>
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| − | | + | |
| − | <!-- Schema 3 -->
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| − |
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| − |
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue mt-4"><strong>Other Experiments</strong></h3>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue pb-1">TA Cloning </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue pb-1">Fusion Cloning</h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row large_image_element mt-4">
| + | |
| − | <div class="col-12">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/4/4f/T--Berlin_diagnostX--toehold_easy.png" class="img-fluid">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <!-- Schema 3.5 -->
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue pb-1"><strong>Mutagenesis</strong></h3>
| + | |
| − | <h5 class="igem_blue pb-1">Überschrift</h5>
| + | |
| − | <p class="text-justify">The synthetic biology experiments described here are particularly important for participation in the iGEM competition. Simultaneously, a second team will work on the recovery of T. solium-RNA from patient stool samples in order to make our test applicable for medical purposes. We are currently forming the necessary partnerships for this goal: A working group in Nairobi has already committed to providing us with relevant material. However, these experiments will be conducted in a properly equipped laboratory, independent from the previously described experiments.</p>
| + | |
| − | </div>
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| − | </div>
| + | |
| − |
| + | |
| − | <!-- Seperator -->
| + | |
| − | <div class="row"><div class="col"><hr class="mt-5 mb-5 igem_seperator mx-auto"></div></div>
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| − | <!-- Schema 2 -->
| + | |
| − |
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue mt-4"><strong>The Idea</strong></h3>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue pb-1">The Tapeworm <em>Taenia solium</em> </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | <div class="col-6">
| + | |
| − | <p class="text-justify">The test will be based on a newly developed method called toehold switch sensors. This method has already been successfully applied to the diagnosis of the Zika virus [3]. We are therefore convinced that toehold switch sensors will also be useful in detecting T. solium.
| + | |
| − | In order to develop our test, we first need to achieve two things:
| + | |
| − | The test will be based on a newly developed method called toehold switch sensors. This method has already been successfully applied to the diagnosis of the Zika virus [3]. We are therefore convinced that toehold switch sensors will also be useful in detecting T. solium.
| + | |
| − | In order to develop our test, we first need to achieve two things:
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <p class="summarybox p-4"><strong>Summary</strong>: Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <!-- Seperator -->
| + | |
| − |
| + | |
| − | <div class="row"><div class="col"><hr class="mt-5 mb-5 igem_seperator mx-auto"></div></div>
| + | |
| − | | + | |
| − | <!-- Schema 3 -->
| + | |
| − |
| + | |
| − |
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue mt-4"><strong>The Experiments</strong></h3>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue pb-1">Production of Toehold Switch Sensors </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue pb-1">Toehold Switch Sensor Test</h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row large_image_element mt-4">
| + | |
| − | <div class="col-12">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/4/4f/T--Berlin_diagnostX--toehold_easy.png" class="img-fluid">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <!-- Seperator -->
| + | |
| − | <div class="row"><div class="col"><hr class="mt-5 mb-5 igem_seperator mx-auto"></div></div>
| + | |
| − | | + | |
| − | <!-- Schema 3.5 -->
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue mt-4"><strong>Application</strong></h3>
| + | |
| − | <h5 class="igem_blue pb-1">Überschrift</h5>
| + | |
| − | <p class="text-justify">The synthetic biology experiments described here are particularly important for participation in the iGEM competition. Simultaneously, a second team will work on the recovery of T. solium-RNA from patient stool samples in order to make our test applicable for medical purposes. We are currently forming the necessary partnerships for this goal: A working group in Nairobi has already committed to providing us with relevant material. However, these experiments will be conducted in a properly equipped laboratory, independent from the previously described experiments.</p>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | </div>
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| | | | |
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| | </div> | | </div> |
| − | <div class="row mt-3">
| |
| − | <div class="col-6">
| |
| − | <h5 class="text-left igem_blue">The Tapeworm <em>Taenia solium</em> </h5>
| |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| |
| − | </div>
| |
| − | <div class="col-6">
| |
| − | <img class="img-fluid" src="images/kitten550x400.jpeg">
| |
| − | </div>
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| | </div> | | </div> |
| − | <div class="row mt-5">
| + | |
| − | <div class="col-6">
| + | |
| − | <img class="img-fluid" src="images/kitten550x400.jpeg">
| + | |
| − | </div>
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue">The Tapeworm <em>Taenia solium</em> </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <!-- Seperator -->
| + | |
| − |
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| − | <div class="row"><div class="col"><hr class="mt-5 mb-5 igem_seperator mx-auto"></div></div>
| + | |
| − | | + | |
| − | <!-- Schema 2 -->
| + | |
| − |
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue mt-4"><strong>The Idea</strong></h3>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue pb-1">The Tapeworm <em>Taenia solium</em> </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | <div class="col-6">
| + | |
| − | <p class="text-justify">The test will be based on a newly developed method called toehold switch sensors. This method has already been successfully applied to the diagnosis of the Zika virus [3]. We are therefore convinced that toehold switch sensors will also be useful in detecting T. solium.
| + | |
| − | In order to develop our test, we first need to achieve two things:
| + | |
| − | The test will be based on a newly developed method called toehold switch sensors. This method has already been successfully applied to the diagnosis of the Zika virus [3]. We are therefore convinced that toehold switch sensors will also be useful in detecting T. solium.
| + | |
| − | In order to develop our test, we first need to achieve two things:
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <p class="summarybox p-4"><strong>Summary</strong>: Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <!-- Seperator -->
| + | |
| − |
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| − | <div class="row"><div class="col"><hr class="mt-5 mb-5 igem_seperator mx-auto"></div></div>
| + | |
| − | | + | |
| − | <!-- Schema 3 -->
| + | |
| − |
| + | |
| − |
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue mt-4"><strong>The Experiments</strong></h3>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue pb-1">Production of Toehold Switch Sensors </h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | <div class="col-6">
| + | |
| − | <h5 class="text-left igem_blue pb-1">Toehold Switch Sensor Test</h5>
| + | |
| − | <p class="text-justify"><em>Taenia solium</em>, otherwise known as pork tapeworm, is a parasite with potentially serious health effects in humans. It infects millions of people worldwide, leading to severe brain diseases, blindness, epilepsy, and death. Countries where where pork production and consumption are coupled with poor hygiene are the worst affected, including large areas of Latin America, Sub-Saharan Africa, and South- and East-Asia, where over 14% of people have experienced an infection at some point <a class="igem_blue" href="#"> [1]</a>. However, a clear picture of how many people are currently infected by T. solium does not exist, due to the current difficulty in making a diagnosis. </p>
| + | |
| − | </div>
| + | |
| − | <div class="row large_image_element mt-4">
| + | |
| − | <div class="col-12">
| + | |
| − | <img src="./images/toeholdswitch_easy.png" class="img-fluid">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <!-- Seperator -->
| + | |
| − | <div class="row"><div class="col"><hr class="mt-5 mb-5 igem_seperator mx-auto"></div></div>
| + | |
| − | | + | |
| − | <!-- Schema 3.5 -->
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-12">
| + | |
| − | <h3 class="text-center igem_blue mt-4"><strong>Application</strong></h3>
| + | |
| − | <h5 class="igem_blue pb-1">Überschrift</h5>
| + | |
| − | <p class="text-justify">The synthetic biology experiments described here are particularly important for participation in the iGEM competition. Simultaneously, a second team will work on the recovery of T. solium-RNA from patient stool samples in order to make our test applicable for medical purposes. We are currently forming the necessary partnerships for this goal: A working group in Nairobi has already committed to providing us with relevant material. However, these experiments will be conducted in a properly equipped laboratory, independent from the previously described experiments.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| | </div> | | </div> |
| | | | |
