Difference between revisions of "Team:Fudan/Composite Part"
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<h class="highlight-title dark-blue"></br></br>For this year’s iGEM competition, our favorite basic part is SynPro(S)-43-8;8* </br>(BBa_K2446028).</h> | <h class="highlight-title dark-blue"></br></br>For this year’s iGEM competition, our favorite basic part is SynPro(S)-43-8;8* </br>(BBa_K2446028).</h> | ||
| − | <p></br>This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor | + | <p></br>This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S). </br></br> |
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<p>The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ end to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats. | <p>The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ end to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats. | ||
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| − | Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website. | + | Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446028">(BBa_K2446028)</a> on the Registry of Standard Biological Parts website. |
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Revision as of 00:33, 1 November 2017
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This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).
The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ end to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.
Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression in constant expression state is high. Meanwhile, it is almost being completely silenced in silencing expression state.
The silencing fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.
Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.
We listed all the composite parts we constructed this year here.
