Difference between revisions of "Team:TecCEM/Basic Part"

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             .textoP{
 
             .textoP{
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                line-height: 1.3em !important;
 
                 font-size: 0.8em !important;
 
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                 <p>Composing Parts: Basic</br></p>             
 
                 <p>Composing Parts: Basic</br></p>             
 
                 <p>Widely used T7 terminator</br></p>
 
                 <p>Widely used T7 terminator</br></p>
−
                 <p>Terminator for parts with T7 promoter (BBa_I712074), a promoter recognized by T7 RNApol, a highly active enzime for a high transcription rate (Canton B., 2008).</br></br></p>              
+
                 <p class = "textoP">Terminator for parts with T7 promoter (BBa_I712074), a promoter recognized by T7 RNApol, a highly active enzime for a high transcription rate (Canton B., 2008).</br></br></p>
 
                 <p class = "textoP">References: Canton B. (2008). Engineering the interface between cellular chassis and synthetic biological systems. Retrieved from https://s3-us-west-2.amazonaws.com/oww-files-public/4/43/Canton-PhD-BE-2008.pdf</p>
 
                 <p class = "textoP">References: Canton B. (2008). Engineering the interface between cellular chassis and synthetic biological systems. Retrieved from https://s3-us-west-2.amazonaws.com/oww-files-public/4/43/Canton-PhD-BE-2008.pdf</p>
 
             </div>
 
             </div>
 
             <div class = "col-md-1"></div>
 
             <div class = "col-md-1"></div>
 
             <div class = "col-md-4 paragraphU cortaTexto">
 
             <div class = "col-md-4 paragraphU cortaTexto">
−
                 <h1 class = "subTitleUbuntu titleh1">BBa_K2246001</h1>
+
                 <h1 class = "subTitleUbuntu titleh1">BBa_K2246002</h1>
 
                 <img class = "centerImage responsiveImage" src="http://placehold.it/200x50">
 
                 <img class = "centerImage responsiveImage" src="http://placehold.it/200x50">
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                 <h2 class = "subTitleUbuntu titleh2">T7 Terminator</h2>
+
                 <h2 class = "subTitleUbuntu titleh2">AWD siRNA</h2>
 
                 <p>Composing Parts: Basic</br></p>                 
 
                 <p>Composing Parts: Basic</br></p>                 
−
                 <p>Widely used T7 terminator</br></p>
+
                 <p>siRNA sequence for silencing Abnormal Wing Disc gene in Diaphorina Citri</br></p>
−
                 <p>Terminator for parts with T7 promoter (BBa_I712074), a promoter recognized by T7 RNApol, a highly active enzime for a high transcription rate (Canton B., 2008).</br></br></p>                 
+
                 <p class = "textoP">This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for Abnormal Wing Disc (AWD) gene in D.citri.  This gene is involved in larvae growth and wing development, when silenced it has been shown to increase nymph mortality and lead to the development of damaged wings, therefore reducing psyllid's ability to fly, thus affecting its movility (El-Shesheny, Hajeri, El-Hawary, Gowda & Killiny, 2013).  The selected target sequence, in order to maximize specificity, was choosen by comparing the sequence of this gene against several species, and an exclusive region from D.citri was selected. Then a BLAST was run in order to eliminate the possibility of hybridization with other species.</br></br></p>                 
 
                 <p class = "textoP">References: Canton B. (2008). Engineering the interface between cellular chassis and synthetic biological systems. Retrieved from https://s3-us-west-2.amazonaws.com/oww-files-public/4/43/Canton-PhD-BE-2008.pdf</p>
 
                 <p class = "textoP">References: Canton B. (2008). Engineering the interface between cellular chassis and synthetic biological systems. Retrieved from https://s3-us-west-2.amazonaws.com/oww-files-public/4/43/Canton-PhD-BE-2008.pdf</p>
 
             </div>
 
             </div>

Revision as of 01:40, 1 November 2017

IGEM_TECCEM

Basic_Parts

Parts

Basic Parts

BBa_K2246001

T7 Terminator

Composing Parts: Basic

Widely used T7 terminator

Terminator for parts with T7 promoter (BBa_I712074), a promoter recognized by T7 RNApol, a highly active enzime for a high transcription rate (Canton B., 2008).

References: Canton B. (2008). Engineering the interface between cellular chassis and synthetic biological systems. Retrieved from https://s3-us-west-2.amazonaws.com/oww-files-public/4/43/Canton-PhD-BE-2008.pdf

BBa_K2246002

AWD siRNA

Composing Parts: Basic

siRNA sequence for silencing Abnormal Wing Disc gene in Diaphorina Citri

This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for Abnormal Wing Disc (AWD) gene in D.citri. This gene is involved in larvae growth and wing development, when silenced it has been shown to increase nymph mortality and lead to the development of damaged wings, therefore reducing psyllid's ability to fly, thus affecting its movility (El-Shesheny, Hajeri, El-Hawary, Gowda & Killiny, 2013). The selected target sequence, in order to maximize specificity, was choosen by comparing the sequence of this gene against several species, and an exclusive region from D.citri was selected. Then a BLAST was run in order to eliminate the possibility of hybridization with other species.

References: Canton B. (2008). Engineering the interface between cellular chassis and synthetic biological systems. Retrieved from https://s3-us-west-2.amazonaws.com/oww-files-public/4/43/Canton-PhD-BE-2008.pdf

IGEM_TECCEM