Difference between revisions of "Team:MSU-Michigan/Current"

 
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<h1>Current from Induction</h1>
 
<h1>Current from Induction</h1>
  
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<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3>
 
<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3>
<img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentmtrB170725.png" style="width:100%">
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<img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentmtrB170725.png" style="width:100%;max-width:600px">
 
<p>Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.</p>
 
<p>Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.</p>
 
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<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3>
 
<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3>
<img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentdeltamrtBGFPinduced.png" style="width=25%; max-width:600px">
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<img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentdeltamrtBGFPinduced.png" style="width=100%; max-width:600px">
 
<p>The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.</p>
 
<p>The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.</p>
 
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<h3>Multiple Strains</h3>
 
<h3>Multiple Strains</h3>
<figure><img src="https://static.igem.org/mediawiki/2017/d/d7/T--MSU-Michigan--current170714.png" width="366.5" height="300">
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<img src="https://static.igem.org/mediawiki/2017/d/d7/T--MSU-Michigan--current170714.png" style="width:100%;max-width:600px">
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<p>Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.</p>
 
<p>Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.</p>
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<h3>Anaerobic Current</h3>
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<figure><img src="https://static.igem.org/mediawiki/2017/2/22/T--MSU-Michigan--currentanaerobic20170911.png" width="366.5" height="300"></figure>
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<p>Anaerobic current showing how current induction can take the place of GFP induction as a marker for gene expression. IPTG induction occurs right at the small dip right around the 2 hour mark.</p>
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<iframe width="560" height="315" src="https://www.youtube.com/embed/91eUc9rtiLA" frameborder="0" allowfullscreen></iframe>
 
<iframe width="560" height="315" src="https://www.youtube.com/embed/91eUc9rtiLA" frameborder="0" allowfullscreen></iframe>
  
 
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{{MSU-Michigan-Footer}}

Latest revision as of 02:11, 1 November 2017

Current from Induction

deltamtrB_GFPmtrB Induced V Uninduced

Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.

deltamtrB_GFPmtrB Induced V Uninduced

The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.

Multiple Strains

Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.

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