Parts

Full Part table

	Part Number	Type	Designer	Length
Nor Pyochelin Synthesis Cluster	BBa_K2401000	Composite	A. Nicolai, S. Ricke	10760 bp
Yersiniabactin inner Membrane Transporter	BBa_K2401005	Composite	A. Nicolai, S. Ricke	3956 bp
Siderophore outer Membrane Transporter	BBa_K2401011	Composite	A. Nicolai, S. Ricke	5724 bp
HMWP1 Yersiniabactin Synthetase part 2	BBa_K2401012	Composite	A. Nicolai, S. Ricke	10731 bp
HMWP2 Yersiniabactin Synthetase Part 1	BBa_K2401001	Basic	A. Nicolai, S. Ricke	6107 bp
2,3-Dihydroxybenzoate-AMP ligase	BBa_K2401002	Basic	A. Nicolai, S. Ricke	1578 bp
Thiazolinyl imide reductase	BBa_K2401003	Basic	A. Nicolai, S. Ricke	1101 bp
Surfactin imide reductase	BBa_K2401004	Basic	A. Nicolai, S. Ricke	498 bp
ABC-Siderophore-Transporter	BBa_K2401006	Basic	A. Nicolai, S. Ricke	1789 bp
ATP-Siderophore-Bindingpermease	BBa_K2401007	Basic	A. Nicolai, S. Ricke	1801 bp
HMWP1 Yersiniabactin Synthetase Part 2	BBa_K2401008	Basic	A. Nicolai, S. Ricke	9492 bp
FyuA TonB Yersiniabactin outermembrane Receptor	BBa_K2401010	Basic	A. Nicolai, S. Ricke	2022 bp
FptA Pyochelin outermembrane Receptor	BBa_K2401009	Basic	A. Nicolai, S. Ricke	2160 bp
PchR Pyochelin regulator	BBa_K2401013	Regulatory	A. Nicolai, S. Ricke	960 bp
AraC like Yersiniabactin regulator	BBa_K2401014	Regulatory	A. Nicolai, S. Ricke	891 bp

Basic Parts

AraC family transcriptional regulator [AraCftr]
Origin:	Escherichia coli
Data:	891 bp, 296 AS, 32,3 kDa
Part No.:	BBa_K2401014
Function:	Yersiniabactin-Complex Regulated Promotor, most likely a leaky promoter that induces the Yersiniabactin synthesis if Yersiniabactin-iron3+-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it.
Plasmid:	Singular on a plasmid (pSB1C3) with cAMP-resistance.

Regulatory protein PchR [PchR]
Origin:	Pseudomonas aeruginosa PAO1 (strain: PAO1)
Data:	960 bp, 319 AS, 35.9 kDa
Part No.:	BBa_K2401013
Function:	Pyochelin-Complex Regulated Promotor, most likely a leaky promoter that induces the Pyochelin synthesis if Pyochelin-iron3+-di-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it. Our goal was to characterize the promoter and measure if pyochelin-gallium3+ and Nor-pyochelin-gallium3+ complexes induce the native promoter to estimate the metabolic excess overload that our antibiotic-compound induces on native Pseudomonas aeruginosa.
Plasmid:	Singular: pSB1C3 with cAMP-resistance. Combination: With HMWP2 , tired, sfp and dhBe to mimic the native expression system for pyochelin to measure additional metabolic load.