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{{NPU-China/mmp}} | {{NPU-China/mmp}} | ||
− | <html lang="en" > | + | <html lang="en"> |
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<head> | <head> | ||
− | <!-- Bootstrap Core CSS --> | + | <!-- Bootstrap Core CSS --> |
− | <link href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" rel="stylesheet"> | + | <link href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" rel="stylesheet"> |
− | <!-- Custom CSS --> | + | <!-- Custom CSS --> |
− | <link href="https://2017.igem.org/Template:NPU-China/css?action=raw&ctype=text/css" rel="stylesheet"> | + | <link href="https://2017.igem.org/Template:NPU-China/css?action=raw&ctype=text/css" rel="stylesheet"> |
− | <!-- Custom Fonts --> | + | <!-- Custom Fonts --> |
− | <link href="https://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet"> | + | <link href="https://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet"> |
− | <!-- jQuery --> | + | <!-- jQuery --> |
− | <script src="https://cdn.bootcss.com/jquery/3.2.1/jquery.min.js"></script> | + | <script src="https://cdn.bootcss.com/jquery/3.2.1/jquery.min.js"></script> |
− | <!-- Bootstrap Core JavaScript --> | + | <!-- Bootstrap Core JavaScript --> |
− | <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js"></script> | + | <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js"></script> |
− | <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries --> | + | <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries --> |
− | <!-- WARNING: Respond.js doesn't work if you view the page via file:// --> | + | <!-- WARNING: Respond.js doesn't work if you view the page via file:// --> |
− | <!--[if lt IE 9]> | + | <!--[if lt IE 9]> |
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− | <!-- Navigation --> | + | <!-- Navigation --> |
− | <nav class="navbar navbar-inverse navbar-fixed-top" role="navigation"> | + | <nav class="navbar navbar-inverse navbar-fixed-top" role="navigation"> |
− | + | <div class="container"> | |
− | + | <div class="navbar-header"> | |
− | + | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> | |
− | + | <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China"> | |
− | + | <img src="https://static.igem.org/mediawiki/2017/2/29/NPU-logo.png" style="max-width:50px;margin-top:-10px;"> | |
− | + | </a> | |
− | + | </div> | |
− | + | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> | |
− | + | <ul class="nav navbar-nav navbar-right"> | |
− | + | <li class="active"> <a href="https://2017.igem.org/Team:NPU-China">Home</a> </li> | |
− | + | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a> | |
− | + | <ul class="dropdown-menu"> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li> | |
− | + | </ul> | |
− | + | </li> | |
− | + | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a> | |
− | + | <ul class="dropdown-menu"> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> </li> | |
− | + | </ul> | |
− | + | </li> | |
− | + | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a> | |
− | + | <ul class="dropdown-menu"> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> </li> | |
− | + | </ul> | |
− | + | </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> </li> | |
− | + | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a> | |
− | + | <ul class="dropdown-menu"> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li> | |
− | + | </ul> | |
− | + | </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li> | |
− | + | ||
− | </ | + | |
− | < | + | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a> |
− | + | <ul class="dropdown-menu"> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> </li> | |
− | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> </li> | |
− | + | </ul> | |
− | + | </li> | |
− | + | </ul> | |
− | + | </div> | |
− | + | </div> | |
− | + | </nav> | |
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− | + | <!-- Header Carousel --> | |
− | + | <header id="myCarousel" class="carousel slide"> | |
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− | + | <div class="carousel-indicators"> | |
− | + | <li data-target="#myCarousel" data-slide-to="0" class="active"></li> | |
− | + | <li data-target="#myCarousel" data-slide-to="1"></li> | |
− | + | <li data-target="#myCarousel" data-slide-to="2"></li> | |
− | </ | + | </div> |
− | <!-- | + | <!-- Wrapper for slides --> |
− | <div class=" | + | <div class="carousel-inner"> |
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− | + | <img src="https://static.igem.org/mediawiki/2017/1/1f/Npu-banner1.jpg"> | |
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− | + | </div> | |
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− | + | <img src="https://static.igem.org/mediawiki/2017/0/06/Npu-banner3.jpg"> | |
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− | + | <img src="https://static.igem.org/mediawiki/2017/2/28/Npu-banner2.jpg"> | |
− | + | </div> | |
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− | + | <a class="left carousel-control" href="#myCarousel" data-slide="prev"> | |
− | + | <span class="icon-prev"></span> | |
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</a> | </a> | ||
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</a> | </a> | ||
− | < | + | <!-- Controls --> |
− | + | </header> | |
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− | </ | + | |
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− | <div class=" | + | <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;"> |
− | + | <!-- Page Content --> | |
− | + | <div class="container"> | |
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− | + | <div class="row" style=" padding-top:70px"> | |
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− | <div class="row"> | + | |
<div class="col-md-12"> | <div class="col-md-12"> | ||
− | < | + | <h2 class="page-header" align="center" >Abstract</h2> |
+ | <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent | ||
+ | polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average | ||
+ | annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion | ||
+ | US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which | ||
+ | is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, | ||
+ | high energy consumption and a lack of sustainablility.<br><br> This year, we aim to use a greener and more environmentally- | ||
+ | friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional | ||
+ | chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene. | ||
+ | </h4> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!-- Marketing Icons Section --> | ||
+ | <div class="row" style="padding-top:70px"> | ||
+ | <div class="col-md-12"> | ||
+ | <h2 class="page-header" align="center">We constructed our cell factory based on 4 levels, which are—</h2> | ||
+ | <br> | ||
</div> | </div> | ||
</div> | </div> | ||
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+ | <div class="row"> | ||
+ | <div class="col-md-6 img-portfolio"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART"> | ||
+ | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png"> | ||
+ | </a> | ||
+ | <h3 align="center"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a> | ||
+ | </h3> | ||
+ | <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type | ||
+ | is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic | ||
+ | effect of ceaS2 enzyme.<br><br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) | ||
+ | platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) | ||
+ | and HTS(High throughput screening). | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div class="col-md-6 img-portfolio"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM"> | ||
+ | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/8/85/System.png" alt=""> | ||
+ | </a> | ||
+ | <h3 align="center"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM">System</a> | ||
+ | </h3> | ||
+ | <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient | ||
+ | to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, | ||
+ | we have E. coli MG1655 and S. cerevisiae BY4741 tested individually. | ||
+ | </h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- /.row --> | ||
+ | <!-- Projects Row --> | ||
+ | <div class="row" style="padding-top:50px"> | ||
+ | <div class="col-md-6 img-portfolio"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY"> | ||
+ | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/e/ec/Pathway.png" alt=""> | ||
+ | </a> | ||
+ | <h3 align="center"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY">Pathway</a> | ||
+ | </h3> | ||
+ | <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different | ||
+ | optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol | ||
+ | metabolism of the two organisms, so that they could produce the target product acrylic acid using the | ||
+ | intermediates G3P and DHAP.<br><br> Besides the construction of the pathways, we also reconstructed | ||
+ | and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | ||
+ | and reduced the loss of by-pass carbon flux.</h4> | ||
+ | </div> | ||
+ | <div class="col-md-6 img-portfolio"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION"> | ||
+ | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/6/67/Production2.png" alt=""> | ||
+ | </a> | ||
+ | <h3 align="center"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a> | ||
+ | </h3> | ||
+ | <h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic | ||
+ | acid. In the subsequent fermentation, we also determined the best conditions | ||
+ | of the engineered microorganism strains.<br><br> Therefore, we selected to control the carbon source, buffer, temperature, pH | ||
+ | and other conditions to optimize the cell production process.</h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- /.container --> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png" class="img-responsive"> | ||
− | <!-- Script to Activate the Carousel --> | + | </div> |
− | <script> | + | <!-- /.batu --> |
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− | </script> | + | |
+ | <!-- Script to Activate the Carousel --> | ||
+ | <script> | ||
+ | $('.carousel').carousel({ | ||
+ | interval: 5000 //changes the speed | ||
+ | }) | ||
+ | </script> | ||
</body> | </body> | ||
+ | |||
</html> | </html> |
Latest revision as of 12:52, 1 November 2017