Lichenyuan (Talk | contribs) |
|||
(49 intermediate revisions by 2 users not shown) | |||
Line 25: | Line 25: | ||
<![endif]--> | <![endif]--> | ||
<style> | <style> | ||
− | |||
</style> | </style> | ||
</head> | </head> | ||
Line 37: | Line 36: | ||
<div class="navbar-header"> | <div class="navbar-header"> | ||
<button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> | ||
− | <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China">NPU- | + | <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China"> |
+ | <img src="https://static.igem.org/mediawiki/2017/2/29/NPU-logo.png" style="max-width:50px;margin-top:-10px;"> | ||
+ | </a> | ||
</div> | </div> | ||
<div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> | ||
Line 44: | Line 45: | ||
<li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a> | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a> | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a> | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> </li> |
<li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a> | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li> |
<li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a> | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> </li> |
− | <li> <a href="https://2017.igem.org/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
Line 105: | Line 106: | ||
</div> | </div> | ||
<div class="item"> | <div class="item"> | ||
− | + | <img src="https://static.igem.org/mediawiki/2017/2/28/Npu-banner2.jpg"> | |
</div> | </div> | ||
</div> | </div> | ||
Line 116: | Line 117: | ||
<!-- Controls --> | <!-- Controls --> | ||
</header> | </header> | ||
− | <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed;"> | + | |
+ | <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;"> | ||
<!-- Page Content --> | <!-- Page Content --> | ||
<div class="container"> | <div class="container"> | ||
− | <div class="row"> | + | <div class="row" style=" padding-top:70px"> |
<div class="col-md-12"> | <div class="col-md-12"> | ||
− | <h2 class="page-header">Abstract</h2> | + | <h2 class="page-header" align="center" >Abstract</h2> |
− | < | + | <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent |
polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average | polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average | ||
annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion | annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion | ||
− | US dollars | + | US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which |
− | is obtained by petroleum cracking) after multi-step treatment | + | is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, |
− | high energy consumption and | + | high energy consumption and a lack of sustainablility.<br><br> This year, we aim to use a greener and more environmentally- |
− | friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional | + | friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional |
− | chemical synthesis methods, Synbio is | + | chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene. |
− | </ | + | </h4> |
+ | |||
</div> | </div> | ||
</div> | </div> | ||
− | + | ||
<!-- Marketing Icons Section --> | <!-- Marketing Icons Section --> | ||
− | <div class="row"> | + | <div class="row" style="padding-top:70px"> |
<div class="col-md-12"> | <div class="col-md-12"> | ||
− | <h2 class="page-header">We | + | <h2 class="page-header" align="center">We constructed our cell factory based on 4 levels, which are—</h2> |
<br> | <br> | ||
</div> | </div> | ||
Line 148: | Line 151: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART"> |
<img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png"> | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png"> | ||
</a> | </a> | ||
− | <h3> | + | <h3 align="center"> |
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a> |
</h3> | </h3> | ||
− | < | + | <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type |
− | We designed | + | is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic |
− | </ | + | effect of ceaS2 enzyme.<br><br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) |
+ | platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) | ||
+ | and HTS(High throughput screening). | ||
+ | </h4> | ||
</div> | </div> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM"> |
− | <img class="img-responsive img-hover" src=" | + | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/8/85/System.png" alt=""> |
</a> | </a> | ||
− | <h3> | + | <h3 align="center"> |
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM">System</a> |
</h3> | </h3> | ||
− | < | + | <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient |
− | </ | + | to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, |
+ | we have E. coli MG1655 and S. cerevisiae BY4741 tested individually. | ||
+ | </h4> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 172: | Line 180: | ||
<!-- Projects Row --> | <!-- Projects Row --> | ||
− | <div class="row"> | + | <div class="row" style="padding-top:50px"> |
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY"> |
− | <img class="img-responsive img-hover" src=" | + | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/e/ec/Pathway.png" alt=""> |
</a> | </a> | ||
− | <h3> | + | <h3 align="center"> |
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY">Pathway</a> |
</h3> | </h3> | ||
− | < | + | <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different |
− | + | optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol | |
+ | metabolism of the two organisms, so that they could produce the target product acrylic acid using the | ||
+ | intermediates G3P and DHAP.<br><br> Besides the construction of the pathways, we also reconstructed | ||
+ | and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | ||
+ | and reduced the loss of by-pass carbon flux.</h4> | ||
</div> | </div> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION"> |
− | <img class="img-responsive img-hover" src=" | + | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/6/67/Production2.png" alt=""> |
</a> | </a> | ||
− | <h3> | + | <h3 align="center"> |
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a> |
</h3> | </h3> | ||
− | < | + | <h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic |
− | Therefore, we selected the carbon source, | + | acid. In the subsequent fermentation, we also determined the best conditions |
+ | of the engineered microorganism strains.<br><br> Therefore, we selected to control the carbon source, buffer, temperature, pH | ||
+ | and other conditions to optimize the cell production process.</h4> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | </div> | ||
+ | <!-- /.container --> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png" class="img-responsive"> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
− | <!-- /. | + | <!-- /.batu --> |
− | + | ||
Latest revision as of 12:52, 1 November 2017