Difference between revisions of "Team:Peking/Notebook"

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{{Peking}}
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<h1>Notebook</h1>
 
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
 
  
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        /***************************************************** DEFAULT WIKI SETTINGS  ****************************************************/
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<h5>What should this page have?</h5>
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<ul>
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<li>Chronological notes of what your team is doing.</li>
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<li> Brief descriptions of daily important events.</li>
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<li>Pictures of your progress. </li>
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<li>Mention who participated in what task.</li>
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</ul>
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<h5>Inspiration</h5>
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<p>You can see what others teams have done to organize their notes:</p>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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        !important;
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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        }
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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            <!-- Title -->
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            <a href="https://2017.igem.org/Team:Peking"><img
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              style="color: #000000; font-size: x-large"><strong>Peking iGEM </strong> 2017</a>
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                <h1 style="font-size: xx-large; color: white;text-shadow:2px 2px 8px #070707;"><strong>Notebook</strong></h1>
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            <section class="docs-toc docs-text-styling">
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            </section>
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            <br>
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            <h2>Week 1</h2>
 +
            <strong>26/06/2017-02/07/2017</strong><br>
 +
            Design primers.<br>
 +
            Construct standard structure of integrase int5 and int8.<br>
 +
            <h2>Week 2</h2>
 +
            <strong>03/07/2017-09/07/2017</strong><br>
 +
            Mutate integrase int8, int10, p22 for Golden Gate Assembly.<br>
 +
            Construct the expression system of integrase TP901, int2, int3, int4, int5, int7, int8, p21, FimB, FimE, HbiF, Vcre, Scre, Cre, Dre, Vika, Lambda-int.<br>
 +
            Construct the reporter system utilized the structure of attB-Terminator-attP.<br>
 +
            <h2>Week 3</h2>
 +
            <strong>10/07/2017-16/07/2017</strong><br>
 +
            Mutate Bxb1-gp35, phiC31 for Golden Gate Assembly.<br>
 +
            Ask for some plasmids of repressilator.<br>
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            <h2>Week 4</h2>
 +
            <strong>17/07/2017-23/07/2017</strong><br>
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            Construct the reporter system of Bxb1, TP901, phiC31.<br>
 +
            Prepare for repressilator test.<br>
 +
            <h2>Week 5</h2>
 +
            <strong>24/07/2017-30/07/2017</strong><br>
 +
            Construct the expression system of integrase TP901, Bxb1-gp35, phiC31 without RBS.<br>
 +
            Design Microfluidic device for the oscillation test of repressilator.<br>
 +
            <h2>Week 6</h2>
 +
            <strong>31/07/2017-06/08/2017</strong><br>
 +
            Construct the expression system of integrase TP901, Bxb1-gp35 with a series RBS of different transcription initiate rate.<br>
 +
            Manufacture seven-segment display microfluidic device.<br>
 +
            <h2>Week 7</h2>
 +
            <strong>07/08/2017-13/08/2017</strong><br>
 +
            Construct the expression system of integrase TP901, Bxb1-gp35 with another series RBS of different transcription initiate rate.<br>
 +
            Image period of LP117 oscillation around 15 hours by microfluidic device.<br>
 +
            <h2>Week 8</h2>
 +
            <strong>14/08/2017-20/08/2017</strong><br>
 +
            Construct the expression system of integrase phiC31 with a series RBS of different transcription initiate rate.<br>
 +
            Manufacture Sand Clock microfluidic device.<br>
 +
            Prepare for parts construction.<br>
 +
            <h2>Week 9</h2>
 +
            <strong>21/08/2017-27/08/2017</strong><br>
 +
            Construct the expression system and reporter system of integrase-RDF (recombination directionality factor) fusion protein.<br>
 +
            Test integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.<br>
 +
            Image period of plpt20 oscillation around 7 hours.<br>
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            <h2>Week 10</h2>
 +
            <strong>28/08/2017-03/09/2017</strong><br>
 +
            Make adjustment for the expression system of integrase TP901, Bxb1-gp35, phiC31 to improve the recombination efficiency, and rebuild the expression system.<br>
 +
            Test the adjusted integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.<br>
 +
            Parts construction of integrase TP901.<br>
 +
            Site mutation of plpt20 original plasmid.<br>
 +
            <h2>Week 11</h2>
 +
            <strong>04/09/2017-10/09/2017</strong><br>
 +
            Construct the front end of Flip-Flop.<br>
 +
            Construct the expression system of integrase-RDF (recombination directionality factor) fusion protein.<br>
 +
            Site mutation of plpt20 original plasmid.<br>
 +
            <h2>Week 12</h2>
 +
            <strong>11/09/2017-17/09/2017</strong><br>
 +
            Make adjustment again for the expression system of integrase TP901, Bxb1-gp35, phiC31 to improve the recombination efficiency, and rebuild the expression system.<br>
 +
            Test the adjusted integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.<br>
 +
            Construct the reporter system of integrase-RDF (recombination directionality factor) fusion protein.<br>
 +
            Parts construction of integrase int2, int5.<br>
 +
            Construct the expression system for oscillator plpt20 with integrase Bxb1-gp35.<br>
 +
            <h2>Week 13</h2>
 +
            <strong>18/09/2017-24/09/2017</strong><br>
 +
            Construct TP901, Bxb1, phiC31 expression system of integrase-RDF.<br>
 +
            Parts construction of integrase expression system.<br>
 +
            Construct the expression system for oscillator plpt20 with integrase T7 promoter.<br>
 +
            <h2>Week 14</h2>
 +
            <strong>25/09/2017-01/10/2017</strong><br>
 +
            Test the recombination efficiency of TP901, Bxb1 integrase-RDF by Flow Cytometer.<br>
 +
            Parts construction of integrase reporter system.<br>
 +
            <h2>Week 15</h2>
 +
            <strong>02/10/2017-08/10/2017</strong><br>
 +
            Make adjustment for integrase-RDF expression system to improve recombination efficiency.<br>
 +
            Test the recombination efficiency of adjusted integrase-RDF.<br>
 +
            Parts construction of integrase expression system and reporter system.<br>
 +
            Construct reporter system for oscillator plpt41 with T7 promoter, Bxb1-gp35.<br>
 +
            Image repressilator-drived DNA recombination.<br>
 +
 +
            <h2>Week 16</h2>
 +
            <strong>09/10/2017-15/10/2017</strong><br>
 +
            Measure the state transition efficiency of the Forward latch.<br>
 +
            Measure the recombination efficiency of the excisionases.<br>
 +
            Parts construction of the integrases and excisionases reporter systems.<br>
 +
            Measure the flipping efficiency and filter efficiency of the terminators flanked by a pair of attB and attP sites.<br>
 +
 +
            <h2>Week 17</h2>
 +
            <strong>16/10/2017-22/10/2017</strong><br>
 +
            Measure the recombination efficiency of the excisionases.<br>
 +
            Parts construction of the integrases and excisionases reporter systems.<br>
 +
            Measure the flipping efficiency and filter efficiency of the terminators flanked by two pair of attB and attP sites.<br>
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Latest revision as of 13:45, 1 November 2017

Peking iGEM 2017

Notebook


Week 1

26/06/2017-02/07/2017
Design primers.
Construct standard structure of integrase int5 and int8.

Week 2

03/07/2017-09/07/2017
Mutate integrase int8, int10, p22 for Golden Gate Assembly.
Construct the expression system of integrase TP901, int2, int3, int4, int5, int7, int8, p21, FimB, FimE, HbiF, Vcre, Scre, Cre, Dre, Vika, Lambda-int.
Construct the reporter system utilized the structure of attB-Terminator-attP.

Week 3

10/07/2017-16/07/2017
Mutate Bxb1-gp35, phiC31 for Golden Gate Assembly.
Ask for some plasmids of repressilator.

Week 4

17/07/2017-23/07/2017
Construct the reporter system of Bxb1, TP901, phiC31.
Prepare for repressilator test.

Week 5

24/07/2017-30/07/2017
Construct the expression system of integrase TP901, Bxb1-gp35, phiC31 without RBS.
Design Microfluidic device for the oscillation test of repressilator.

Week 6

31/07/2017-06/08/2017
Construct the expression system of integrase TP901, Bxb1-gp35 with a series RBS of different transcription initiate rate.
Manufacture seven-segment display microfluidic device.

Week 7

07/08/2017-13/08/2017
Construct the expression system of integrase TP901, Bxb1-gp35 with another series RBS of different transcription initiate rate.
Image period of LP117 oscillation around 15 hours by microfluidic device.

Week 8

14/08/2017-20/08/2017
Construct the expression system of integrase phiC31 with a series RBS of different transcription initiate rate.
Manufacture Sand Clock microfluidic device.
Prepare for parts construction.

Week 9

21/08/2017-27/08/2017
Construct the expression system and reporter system of integrase-RDF (recombination directionality factor) fusion protein.
Test integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.
Image period of plpt20 oscillation around 7 hours.

Week 10

28/08/2017-03/09/2017
Make adjustment for the expression system of integrase TP901, Bxb1-gp35, phiC31 to improve the recombination efficiency, and rebuild the expression system.
Test the adjusted integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.
Parts construction of integrase TP901.
Site mutation of plpt20 original plasmid.

Week 11

04/09/2017-10/09/2017
Construct the front end of Flip-Flop.
Construct the expression system of integrase-RDF (recombination directionality factor) fusion protein.
Site mutation of plpt20 original plasmid.

Week 12

11/09/2017-17/09/2017
Make adjustment again for the expression system of integrase TP901, Bxb1-gp35, phiC31 to improve the recombination efficiency, and rebuild the expression system.
Test the adjusted integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.
Construct the reporter system of integrase-RDF (recombination directionality factor) fusion protein.
Parts construction of integrase int2, int5.
Construct the expression system for oscillator plpt20 with integrase Bxb1-gp35.

Week 13

18/09/2017-24/09/2017
Construct TP901, Bxb1, phiC31 expression system of integrase-RDF.
Parts construction of integrase expression system.
Construct the expression system for oscillator plpt20 with integrase T7 promoter.

Week 14

25/09/2017-01/10/2017
Test the recombination efficiency of TP901, Bxb1 integrase-RDF by Flow Cytometer.
Parts construction of integrase reporter system.

Week 15

02/10/2017-08/10/2017
Make adjustment for integrase-RDF expression system to improve recombination efficiency.
Test the recombination efficiency of adjusted integrase-RDF.
Parts construction of integrase expression system and reporter system.
Construct reporter system for oscillator plpt41 with T7 promoter, Bxb1-gp35.
Image repressilator-drived DNA recombination.

Week 16

09/10/2017-15/10/2017
Measure the state transition efficiency of the Forward latch.
Measure the recombination efficiency of the excisionases.
Parts construction of the integrases and excisionases reporter systems.
Measure the flipping efficiency and filter efficiency of the terminators flanked by a pair of attB and attP sites.

Week 17

16/10/2017-22/10/2017
Measure the recombination efficiency of the excisionases.
Parts construction of the integrases and excisionases reporter systems.
Measure the flipping efficiency and filter efficiency of the terminators flanked by two pair of attB and attP sites.






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