Difference between revisions of "Team:Uppsala/Improve"

(Prototype team page)
 
 
(8 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
{{Uppsala}}
+
{{Uppsala/CSS}}
−
<html>
+
{{Uppsala/Navbar}}
  
 +
<!DOCTYPE html>
 +
<html lang="en">
 +
<head>
 +
  <title>Results</title>
 +
  <meta charset="utf-8">
 +
  <meta name="viewport" content="width=device-width, initial-scale=1">
 +
  <link href="https://fonts.googleapis.com/css?family=Raleway" rel="stylesheet">
 +
  <link href="https://fonts.googleapis.com/css?family=Roboto+Slab" rel="stylesheet">
 +
  <style type="text/css">
 +
    .background {
 +
      background-image: url("https://static.igem.org/mediawiki/2017/f/f9/Results_background.svg");
 +
      background-repeat: repeat-y;
 +
      background-position: center;
 +
      background-size: 100%;
 +
    }
  
−
<div class="column full_size judges-will-not-evaluate">
+
    .textbox {
−
<h3>★  ALERT! </h3>
+
      font-family: "Roboto Slab";
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
      font-size: 3vh;
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
      background-image: url("https://static.igem.org/mediawiki/2017/5/5b/Results_text_background.svg");
−
</div>
+
      padding: 80px;
−
<div class="clear"></div>
+
      text-align: center;
 +
line-height:150%;
  
−
<div class="column full_size">
+
    }
−
<h1>Improve</h1>
+
−
<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
+
  
−
<h3>Gold Medal Criterion #2</h3>
+
    .header {
−
<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
+
    font-family: "Raleway";
 +
    text-align: center;
 +
font-size: 5vh;
 +
padding-bottom: 2%;
 +
line-height:150%;
 +
    }
  
−
<br><br>
+
  </style>
−
<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
+
</head>
  
 +
<body>
  
 +
<div class="container-fluid background">
 +
 +
  <div class="row">
 +
    <div class="col-xs-1"></div>
 +
    <div class="col-xs-10 textbox">
 +
      <div class="header" style="margin-top:100px;">Improvement</div>
 +
      <img src="https://static.igem.org/mediawiki/2017/3/30/Results_line_under_title.svg" style="margin: auto;">
 +
      <div class="textbox">Our project was in a sense, a continuation of Uppsala 2013 and Slovenia 2010 team's legacy. We have worked with the Slovenia 2010 strain, and integrated the genes into the chromosome to stabilize production. We improved the crocin  biosynthetic pathway that Uppsala 2013 had problems with by identifying and working with the correct genes in the pathway i.e CaCCD2 and CsADH2946. We have done codon optimization of all the enzyme sequences and created new biobricks with them. Another improvement we made was to add different promoters to the biobrick. In all of our enzymes, we have worked with both constitutive and inducible promoters. Since our enzymes were poorly characterized , we added a his-tag to them to make the purification more achievable. The addition of his-tag was done after consulting the structural models of the enzymes. For example, <a href="http://parts.igem.org/Part:BBa_K2423008">BBa_K2423008</a> is an improved version of <a href="http://parts.igem.org/Part:BBa_K1033112">BBa_K1033112</a> and a slightly modified version of <a href="http://parts.igem.org/Part:BBa_K2423002">BBa_K2423002</a> (different promoter and RBS).
 
</div>
 
</div>
  
  
  
 +
      <img src="https://static.igem.org/mediawiki/2017/0/07/Results_title_footer.svg" style="margin: auto; width: 60%; padding-top: 20px;" class="img-responsive" align="middle">
 +
    </div>
 +
    <div class="col-xs-1"></div>
 +
  </div>   
 +
 +
</div>
 +
 +
</body>
 
</html>
 
</html>
 +
 +
{{Uppsala/Footer}}

Latest revision as of 13:51, 1 November 2017

<!DOCTYPE html> Results

Improvement
Our project was in a sense, a continuation of Uppsala 2013 and Slovenia 2010 team's legacy. We have worked with the Slovenia 2010 strain, and integrated the genes into the chromosome to stabilize production. We improved the crocin biosynthetic pathway that Uppsala 2013 had problems with by identifying and working with the correct genes in the pathway i.e CaCCD2 and CsADH2946. We have done codon optimization of all the enzyme sequences and created new biobricks with them. Another improvement we made was to add different promoters to the biobrick. In all of our enzymes, we have worked with both constitutive and inducible promoters. Since our enzymes were poorly characterized , we added a his-tag to them to make the purification more achievable. The addition of his-tag was done after consulting the structural models of the enzymes. For example, BBa_K2423008 is an improved version of BBa_K1033112 and a slightly modified version of BBa_K2423002 (different promoter and RBS).