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Revision as of 14:03, 1 November 2017
The fight against T. solium
The worldwide fight against T.solium is facing three different challenges
HIGH PREVALENCE
Estimated billions are affected, the infection causing epilepsy, blindness and death
INSUFFICIENT DRUG DISTRIBUTION
Although affordable therapeutics exit
EXPENSIVE DIAGNOSTICS
No method suited for field use
The vision of diagnost-x
Developing a low cost, easy-to-handle diagnostic test for infection with the tapeworm Taenia solium
Target Identification
Having 96 million RNAs sequenced and mapped them against about 12.000 Genes of T. saginata T. asiatica and T. solium, the expression level of unique RNAs was analyzed.
We identified 215 unique, potential diagnost-x targets.
The colour intensity is proportional to the expression of each identified possible target.
First direct RNA isolation (blau) from T. solium eggs without a manipulative hatching procedure and thereof performed transcriptome analysis
Sensor Design
Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.
Refined preselection of promising sensors thanks to established software
Sensor Screening
The designed switches from the In Silico Team were furthermore assembled using Extension PCR with for every switch tailored primers. Assembled Sensors passed all through the standard screening in liquid phase.
6 successfully working sensors detected thanks to our high flow-through Screening Pipeline
Route to the future
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Target Identification
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