Shuimoliuyun (Talk | contribs) |
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<br>At present, the method mainly uses Clostridium propionicum as the starting strain. | <br>At present, the method mainly uses Clostridium propionicum as the starting strain. | ||
− | Specific metabolic pathway is shown in | + | Specific metabolic pathway is shown in the following picture. Although the route is short, |
but there are still many problems when used in producing acrylic acid. | but there are still many problems when used in producing acrylic acid. | ||
<br> | <br> | ||
− | <br>As can be seen from | + | <br>As can be seen from the picture, metabolism of lactic acid is divided into the left |
side, the oxidation pathway and the right side, the reduction pathway. When Clostridium | side, the oxidation pathway and the right side, the reduction pathway. When Clostridium | ||
propionate uses lactic acid as a source of energy, one molecule of lactic acid produces | propionate uses lactic acid as a source of energy, one molecule of lactic acid produces | ||
− | one third of the molecule of acetic acid and 2/3 of the propionic acid | + | one third of the molecule of acetic acid and 2/3 of the propionic acid. In the |
production process of acetic acid, there will be four electrons and 4 protons generate, | production process of acetic acid, there will be four electrons and 4 protons generate, | ||
as well as offering ATP for bacteria to grow. The acryloyl CoA in the reduction pathway | as well as offering ATP for bacteria to grow. The acryloyl CoA in the reduction pathway | ||
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<div class="panel-body"> | <div class="panel-body"> | ||
3-hydroxy propionic acid (3-HP) is an important chemical intermediate, there are a number of patents have reported the methods | 3-hydroxy propionic acid (3-HP) is an important chemical intermediate, there are a number of patents have reported the methods | ||
− | of sugar conversion to 3-HP | + | of sugar conversion to 3-HP. And 3-HP has two functional groups, carboxyl |
and hydroxyl. It is possible to synthesize a variety of important chemical substances | and hydroxyl. It is possible to synthesize a variety of important chemical substances | ||
such as 1,3-propanediol, succinic acid through oxidation, dehydration and esterification | such as 1,3-propanediol, succinic acid through oxidation, dehydration and esterification | ||
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<br> Using 3-hydroxypropionic acid-related pathways to construct engineering bacteria | <br> Using 3-hydroxypropionic acid-related pathways to construct engineering bacteria | ||
− | to produce acrylic acid is a hotspot in recent years | + | to produce acrylic acid is a hotspot in recent years, but the yield is generally |
not high, partly because of the toxic side effects of acrylic acid on cells and, | not high, partly because of the toxic side effects of acrylic acid on cells and, | ||
on the other hand, the pathway is long, and the reaction requires vitamin B12 to | on the other hand, the pathway is long, and the reaction requires vitamin B12 to | ||
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<div class="panel-body"> | <div class="panel-body"> | ||
Dimethylsulphoniopropionate (DMSP) is a sulfur metabolite synthesized in some higher plants in sea water, algae and marine | Dimethylsulphoniopropionate (DMSP) is a sulfur metabolite synthesized in some higher plants in sea water, algae and marine | ||
− | microalgae | + | microalgae. Some marine organisms such as bacteria, phytoplankton have DMSP |
− | lyase, which cleaves the DMSP into acrylate and dimethyl sulfide (DMS) | + | lyase, which cleaves the DMSP into acrylate and dimethyl sulfide (DMS), which |
is also commonly found in Alcaligines faecalis, The route is shown in Figure 1.3. | is also commonly found in Alcaligines faecalis, The route is shown in Figure 1.3. | ||
Extracellular DMSP is transported into the body by the carrier protein, be cleaved | Extracellular DMSP is transported into the body by the carrier protein, be cleaved |
Latest revision as of 14:08, 1 November 2017