Difference between revisions of "Team:Arizona State/Results"

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<h2>Sender Quest, Battle of the AHLs</h2>
 
<h2>Sender Quest, Battle of the AHLs</h2>
<p>Some of the specific questions this research was aiming to answer were: How do combinations of senders affect gene output? Can I find any combinations of senders that increase the overall GFP expression? Can I find any combinations that do not affect the GFP expression? </p>
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<p>Some of the specific questions this research was aiming to answer were: How do combinations of senders affect gene output? Are there any combinations of senders that increase the overall GFP expression? Are there any combinations that do not affect the GFP expression? </p>
  
<p>The specific senders I have chosen for the induction tests were selected because previous research showed that they have either a very low or very high rate of GFP induction when used in a single sender/ receiver circuit. In other words, the chosen senders tend to either work very well or not very well at all and we need more data on how well these senders express the gene when used in combination with another. By combining two senders at a time, sometimes with senders that have shown to induce a high GFP expression and sometimes with senders that have shown a weak induction, I wanted to see if I could increase or decrease the GFP expression on demand as needed. The controls I used for the experiment were testing single sender inductions on the same plate as the combinations, the use of blank wells (LB AMP 100%), a positive GFP control, and a negative control with negative receiver cells and negative sender supernatant.</p>
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<p>The specific senders that were chosen for the induction tests were selected because previous research showed that they have either a very low or very high rate of GFP induction when used in a single sender/ receiver circuit. In other words, the chosen senders tend to either work very well or not very well at all. More data is needed on how well these senders express the gene when used in combination with another. By combining two senders at a time, sometimes with senders that have shown to induce a high GFP expression and sometimes with senders that have shown a weak induction, the goal is to see if there is any increase or decrease the GFP expression on demand. The controls used for the experiment were single sender inductions on the same plate as the combinations, the use of blank wells (LB AMP 100%), a positive GFP control, and a negative control with negative receiver cells and negative sender supernatant.</p>
 
<p>The first sets of tests that utilize the LuxR were duplicates from last years results, there were some discrepancies in their final data so, all tests were duplicated to ensure the results were accurate before moving forward with the testing of further receivers. </p>
 
<p>The first sets of tests that utilize the LuxR were duplicates from last years results, there were some discrepancies in their final data so, all tests were duplicated to ensure the results were accurate before moving forward with the testing of further receivers. </p>
 
<p>Additional information regarding error calculations and additional graphs for the data presented below is available in the results summary section. </p>
 
<p>Additional information regarding error calculations and additional graphs for the data presented below is available in the results summary section. </p>

Revision as of 16:03, 1 November 2017