Difference between revisions of "Team:NTHU Taiwan/Contribution"

 
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</head>
 
</head>
  
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<!-- <div class="munu_img">
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan">
 
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<img src="https://static.igem.org/mediawiki/2017/1/1f/T--NTHU_Taiwan--HOME--logo.png">
 
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</a>
 
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MENU 
 
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<div id="menu_content">
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan">
 
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HOME
 
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</a>
 
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<button class="menu_button">TEAM</button>
 
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<div class="submenu_button">
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Team">
 
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<div id="Team_page">
 
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Team
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Attributions">
 
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<div  id="Attributions_page">
 
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Attributions
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Collaborations">
 
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<div  id="Collaborations_page">
 
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Collaborations
 
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</a>
 
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Description
 
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</a>
 
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<div  id="Design_page">
 
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Design
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Contribution">
 
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<div  id="Contribution_page">
 
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Contribution
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Model">
 
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<div  id="Model_page">
 
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Model
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Results">
 
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<div  id="Results_page">
 
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Results
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Demonstrate">
 
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<div  id="Demonstrate_page">
 
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Demonstrate
 
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</div>
 
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</a>
 
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</div>
 
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</div>
 
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<div class="submenu_wrapper">
 
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<button class="menu_button">WET LAB</button>
 
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<div class="submenu_button">
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Experiments">
 
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<div  id="Experiments_page">
 
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Experiments
 
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</div>
 
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</a>
 
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<a href="#">
 
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<div  id="Protocol_page">
 
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Protocol
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Notebook">
 
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<div  id="Notebook_page">
 
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Notebook
 
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</div>
 
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</a>
 
−
<a href="https://2017.igem.org/Team:NTHU_Taiwan/InterLab">
 
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<div  id="InterLab_page">
 
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InterLab
 
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</div>
 
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</a>
 
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</div>
 
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</div>
 
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<div class="submenu_wrapper">
 
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<button class="menu_button">PARTS</button>
 
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<div class="submenu_button">
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Basic_Part">
 
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<div  id="Basic_Part_page">
 
−
Basic Parts
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Composite_Part">
 
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<div  id="Composite_Part_page">
 
−
Composite Parts
 
−
</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Part_Collection">
 
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<div  id="Part_Collection_page">
 
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Part Collection
 
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</div>
 
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</a>
 
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</div>
 
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</div>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Safety">
 
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<div class="menu_button">
 
−
SAFETY
 
−
</div>
 
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</a>
 
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<div class="submenu_wrapper">
 
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<button class="menu_button">HUMAN PRACTICES</button>
 
−
<div class="submenu_button">
 
−
<a href="https://2017.igem.org/Team:NTHU_Taiwan/HP/Silver">
 
−
<div id="Silver_page">
 
−
Silver HP
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/HP/Gold_Integrated">
 
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<div  id="Gold_Integrated_page">
 
−
Integrated and Gold
 
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</div>
 
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</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Engagement">
 
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<div  id="Engagement_page">
 
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Public Engagement
 
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</div>
 
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</a>
 
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</div>
 
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</div>
 
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<div class="submenu_wrapper">
 
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<button class="menu_button">AWARDS</button>
 
−
<div class="submenu_button">
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Applied_Design">
 
−
<div id="Applied_Design_page">
 
−
Applied Design
 
−
</div>
 
−
</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Entrepreneurship">
 
−
<div  id="Entrepreneurship_page">
 
−
Integrated Human Practices
 
−
</div>
 
−
</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Hardware">
 
−
<div  id="Hardware_page">
 
−
Hardware
 
−
</div>
 
−
</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Model">
 
−
<div  id="Model_page">
 
−
Model
 
−
</div>
 
−
</a>
 
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<a href="https://2017.igem.org/Team:NTHU_Taiwan/Software">
 
−
<div  id="Software_page">
 
−
Software
 
−
</div>
 
−
</a>
 
−
<a href="#">
 
−
<div>
 
−
Criteria
 
−
</div>
 
−
</a>
 
−
</div>
 
−
</div>
 
−
 
−
<a href="https://igem.org/2017_Judging_Form?team=NTHU_Taiwan">
 
−
<div class="menu_button">
 
−
JUDGING FORM
 
−
</div>
 
−
</a>
 
−
 
−
</div>
 
−
 
−
</div>
 
  
  
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<!-- start of content -->
 
<!-- start of content -->
 
<div class="igem_2017_content_wrapper">
 
<div class="igem_2017_content_wrapper">
−
<img width="25%" src="https://static.igem.org/mediawiki/2017/e/e2/T--NTHU_Taiwan--Notebook--Top.png">
+
<img width="25%" src="https://static.igem.org/mediawiki/2017/3/3d/T--NTHU_Taiwan--Project--gear.png">
 
<div style="text-align: center">
 
<div style="text-align: center">
 
<h1 style="color:#DF6A6A">Contribution
 
<h1 style="color:#DF6A6A">Contribution
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<hr width="20%" />
 
<hr width="20%" />
 
</div>
 
</div>
 +
<div class="content">
 +
   
 +
<h2>
 +
Interlab measurement study
 +
</h2>
  
−
<style>
 
−
p{
 
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width:1000px;
 
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font-size:15px;
 
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text-align: justify;
 
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text-justify:inter-ideograph;
 
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line-height: 30px;
 
−
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</style>
 
−
<body>
 
−
<center>
 
  
 +
<p>
 +
We have finished the interlab experiments and get lots of results in <a href="https://2017.igem.org/Team:NTHU_Taiwan/InterLab">the page of interlab.</a>
 +
</p><br><br><br>
  
  
−
<p>
+
<h2>
−
1. interlab measurement study
+
Contribution of new parts
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</p>
+
</h2>
  
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<p>
 
−
We have finished the interlab experiments and get lots of results in the page of interlab
 
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</p>
 
  
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<p>
 
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2. Contribution of new parts
 
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</p>
 
  
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<p>
+
<p>
−
We have contributed new 12 biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Figure 1)
+
We have contributed 12 new biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Table 1)
−
</p>
+
</p><br>
  
−
<p>
 
−
Figure 1. the list of our new 12 parts
 
−
</p>
 
  
−
<p>
+
<table style="border:3px #cccccc solid;" cellpadding="10" border='1';"font-size:18px";"font-family:Comic Sans MS">
−
3. Validation of composite parts’ function
+
　<tr>
−
</p>
+
　<td style="background-color:#99c2ff">biobrick</td>
 +
　<td style="background-color:#99c2ff">type</td>
 +
  <td style="background-color:#99c2ff">name</td>
 +
  <td style="background-color:#99c2ff">description</td>
 +
  <td style="background-color:#99c2ff">designer</td>
 +
  <td style="background-color:#99c2ff">length</td>
 +
　</tr>
 +
　<tr>
 +
  <td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354000">K2354000</a></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">HRP</td>
 +
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">927</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354001">K2354001</a></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">Laccase BP</td>
 +
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">1572</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354002">K2354002</a></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">Laccase SC</td>
 +
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">1539</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354003">K2354003</a></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">Laccase TC</td>
 +
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">1593</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354004">K2354004</a></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">Laccase TV</td>
 +
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">1557</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354005">K2354005</a></td></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">monobody TV</td>
 +
  <td style="background-color:#ffe680">Bind to ER alpha when ER alpha is bind with EDCs</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">435</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354006">K2354006</a></td></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">MNP TV</td>
 +
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">1095</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354007">K2354007</a></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">ER-alpha TV</td>
 +
  <td style="background-color:#ffe680">Bind to EDCs</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">759</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354008">K2354008</a></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">monobody</td>
 +
  <td style="background-color:#ffe680">Bind to ER alpha when ER alpha is bind with EDCs</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">288</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354009">K2354009</a></td>
 +
　<td style="background-color:#ffe680">basic</td>
 +
  <td style="background-color:#ffe680">ER-alpha HS</td>
 +
  <td style="background-color:#ffe680">Bind to EDCs</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">897</td>
 +
　</tr>
 +
　<tr>
 +
  <td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354010">K2354010</a></td>
 +
　<td style="background-color:#ffe680">composite</td>
 +
  <td style="background-color:#ffe680">monobody-GBP HS</td>
 +
  <td style="background-color:#ffe680">Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface.</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">797</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354011">K2354011</a></td>
 +
　<td style="background-color:#ffe680">composite</td>
 +
  <td style="background-color:#ffe680">INP-ER-alpha HS </td>
 +
  <td style="background-color:#ffe680">Expression of ER-alpha on the membrane of <I>E. coli</I> and ER-alpha can bind to EDCs</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">2199</td>
 +
　</tr>
 +
　<tr>
 +
　<td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354012">K2354012</a></td>
 +
　<td style="background-color:#ffe680">composite</td>
 +
  <td style="background-color:#ffe680">HRP-his tag</td>
 +
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">1217</td>
 +
　</tr>
 +
</table>
  
−
<p>
+
<p><center><font size="2">
−
We constructed three composite parts and all of their function have been validated:
+
Table 1. the list of our 12 new parts
−
</p>
+
</font></center></p><br><br><br>
  
−
<p>
+
<h2>
−
(1)Part:BBa_K2354010:monobody-GBP HS
+
Validation of composite parts’ function
−
</p>
+
</h2>
  
−
<p>
 
−
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it and binds to ER-alpha/EDCs complex.
 
−
</p>
 
  
−
<p>
+
<p>
−
(2)Part:BBa_K2354011:INP-ER-alpha HS
+
We constructed three composite parts and all of their function have been validated:
−
</p>
+
</p>
  
−
<p>
 
−
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
 
−
</p>
 
  
−
<p>
+
<p>
−
(3)Part:BBa_K2354012:HRP-his tag
+
(1)
−
</p>
+
<a href="http://parts.igem.org/Part:BBa_K2354010">Part:BBa_K2354010</a>
 +
:monobody-GBP HS
 +
</p>
  
−
<p>
+
<p>
−
Horseradish peroxidase is one kind of peroxidase which has heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
+
<img width="65%" src="https://static.igem.org/mediawiki/2017/7/71/T--NTHU_Taiwan--Parts--10.png">
−
</p>
+
</p>
  
−
</center>
 
−
</body>
 
  
 +
<p>
 +
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it can thus capture our ER-alpha/EDCs complex.
 +
</p><br><br>
  
−
</div>
 
  
−
<script type="Text/JavaScript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.6/jquery.min.js"></script>
+
<p>
 +
(2)
 +
<a href="http://parts.igem.org/Part:BBa_K2354011">Part:BBa_K2354011</a>
 +
:INP-ER-alpha HS
 +
</p>
  
−
<script>
+
<p>
 +
<img width="65%" src="https://static.igem.org/mediawiki/2017/e/eb/T--NTHU_Taiwan--Parts--k11.png">
 +
</p>
  
−
// This is the jquery part of your template. 
 
−
// Try not modify any of this code too much since it makes your menu work.
 
  
−
$(document).ready(function() {
+
<p>
 +
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of <I>E. coli</I> via the function of ice nucleating protein and can bind to EDCs for detection via  ER-alpha.
 +
</p><br><br>
  
−
$("#HQ_page").attr('id','');
 
  
−
// call the functions that control the menu
+
<p>
−
menu_functionality();
+
(3)
−
hide_show_menu();
+
<a href="http://parts.igem.org/Part:BBa_K2354012">Part:BBa_K2354012</a>
 +
:HRP-his tag
 +
</p>
  
 +
<p>
 +
<img width="48%" src="https://static.igem.org/mediawiki/2017/0/06/T--NTHU_Taiwan--Parts-k12.png">
 +
</p>
  
  
−
//this function controls the expand and collapse buttons of the menu and changes the +/- symbols
+
<p>
−
function menu_functionality() {
+
Horseradish peroxidase is a kind of peroxidase which uses heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
 +
</p>
  
−
//when clicking on a "menu_button", it will change the "+/-" accordingly and it will show/hide the corresponding submenu
 
−
$(".menu_button").click(function(){
 
  
−
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−
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$("#display_menu_control").click(function(){
 
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$('#menu_content').fadeToggle(400);
 
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// select a page from the menu based on the id assigned to it and the current page name and add the class "current page" to make it change background color
 
−
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−
 
−
// now that the current_page class has been added to a menu item, make the submenu fade in
 
−
$(".current_page").parents(".submenu_wrapper").fadeIn(400);
 
−
// change the +/- symbol of the corresponding menu button
 
−
$(".current_page").parents(".submenu_wrapper").prev().children().toggleClass("open");
 
−
 
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−
 
−
 
−
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−
function hide_show_menu() {
 
 
 
−
// in case you preview mode is selected, the menu is hidden for better visibility
 
−
if (window.location.href.indexOf("submit") >= 0) {
 
−
$(".igem_2017_menu_wrapper").hide();
 
−
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−
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−
// when the "bars_item" has been clicked
+
</div>
−
$("#bars_item").click(function() {
+
</div>
−
$("#sideMenu").hide();
+
−
 
+
−
// show/hide the menu wrapper
+
−
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Latest revision as of 16:06, 1 November 2017

HOME
Team
Attributions
Collaborations
Description
Design
Contribution
Model
Results
Demonstrate
Experiments
Protocol
Notebook
InterLab
Basic Parts
Composite Parts
Part Collection
SAFETY
Silver HP
Integrated and Gold
Public Engagement
Applied Design
Integrated Human Practices
Hardware
Model
Software
Criteria
JUDGING FORM

Contribution

Interlab measurement study

We have finished the interlab experiments and get lots of results in the page of interlab.




Contribution of new parts

We have contributed 12 new biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Table 1)


　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　
biobricktype name description designer length
K2354000basic HRP Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 927
K2354001basic Laccase BP Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1572
K2354002basic Laccase SC Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1539
K2354003basic Laccase TC Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1593
K2354004basic Laccase TV Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1557
K2354005basic monobody TV Bind to ER alpha when ER alpha is bind with EDCs Chan, Wei-Jen 435
K2354006basic MNP TV Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1095
K2354007basic ER-alpha TV Bind to EDCs Chan, Wei-Jen 759
K2354008basic monobody Bind to ER alpha when ER alpha is bind with EDCs Chan, Wei-Jen 288
K2354009basic ER-alpha HS Bind to EDCs Chan, Wei-Jen 897
K2354010composite monobody-GBP HS Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface. Chan, Wei-Jen 797
K2354011composite INP-ER-alpha HS Expression of ER-alpha on the membrane of E. coli and ER-alpha can bind to EDCs Chan, Wei-Jen 2199
K2354012composite HRP-his tag Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1217

Table 1. the list of our 12 new parts




Validation of composite parts’ function

We constructed three composite parts and all of their function have been validated:

(1) Part:BBa_K2354010 :monobody-GBP HS

Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it can thus capture our ER-alpha/EDCs complex.



(2) Part:BBa_K2354011 :INP-ER-alpha HS

ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E. coli via the function of ice nucleating protein and can bind to EDCs for detection via ER-alpha.



(3) Part:BBa_K2354012 :HRP-his tag

Horseradish peroxidase is a kind of peroxidase which uses heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.