(13 intermediate revisions by 5 users not shown) | |||
Line 20: | Line 20: | ||
display:block; | display:block; | ||
float:left; | float:left; | ||
− | font-family:' | + | font-family:'Helvetica', sans-serif; |
background-color: #F6F6E3; | background-color: #F6F6E3; | ||
} | } | ||
Line 39: | Line 39: | ||
.igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 { | .igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 { | ||
+ | font-family: Helvetica; | ||
padding: 50px 0px 15px 0px; | padding: 50px 0px 15px 0px; | ||
border-bottom: 0px; | border-bottom: 0px; | ||
Line 52: | Line 53: | ||
.igem_2017_content_wrapper .content p{ | .igem_2017_content_wrapper .content p{ | ||
+ | font-family: Helvetica; | ||
color: #2C2C2C; | color: #2C2C2C; | ||
font-size: 1.4em; | font-size: 1.4em; | ||
Line 59: | Line 61: | ||
text-justify:inter-ideograph; | text-justify:inter-ideograph; | ||
} | } | ||
+ | |||
.igem_2017_content_wrapper .content img{ | .igem_2017_content_wrapper .content img{ | ||
Line 104: | Line 107: | ||
<!-- start of content --> | <!-- start of content --> | ||
<div class="igem_2017_content_wrapper"> | <div class="igem_2017_content_wrapper"> | ||
+ | <img width="25%" src="https://static.igem.org/mediawiki/2017/3/3d/T--NTHU_Taiwan--Project--gear.png"> | ||
<div style="text-align: center"> | <div style="text-align: center"> | ||
<h1 style="color:#DF6A6A">Contribution | <h1 style="color:#DF6A6A">Contribution | ||
Line 113: | Line 117: | ||
<h2> | <h2> | ||
− | + | Interlab measurement study | |
</h2> | </h2> | ||
<p> | <p> | ||
− | We have finished the interlab experiments and get lots of results in the page of interlab. | + | We have finished the interlab experiments and get lots of results in <a href="https://2017.igem.org/Team:NTHU_Taiwan/InterLab">the page of interlab.</a> |
− | </p> | + | </p><br><br><br> |
<h2> | <h2> | ||
− | + | Contribution of new parts | |
</h2> | </h2> | ||
Line 130: | Line 134: | ||
<p> | <p> | ||
We have contributed 12 new biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Table 1) | We have contributed 12 new biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Table 1) | ||
− | </p> | + | </p><br> |
Line 228: | Line 232: | ||
<td style="background-color:#ffe680">Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface.</td> | <td style="background-color:#ffe680">Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface.</td> | ||
<td style="background-color:#ffe680">Chan, Wei-Jen</td> | <td style="background-color:#ffe680">Chan, Wei-Jen</td> | ||
− | <td style="background-color:#ffe680"> | + | <td style="background-color:#ffe680">797</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 236: | Line 240: | ||
<td style="background-color:#ffe680">Expression of ER-alpha on the membrane of <I>E. coli</I> and ER-alpha can bind to EDCs</td> | <td style="background-color:#ffe680">Expression of ER-alpha on the membrane of <I>E. coli</I> and ER-alpha can bind to EDCs</td> | ||
<td style="background-color:#ffe680">Chan, Wei-Jen</td> | <td style="background-color:#ffe680">Chan, Wei-Jen</td> | ||
− | <td style="background-color:#ffe680"> | + | <td style="background-color:#ffe680">2199</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 244: | Line 248: | ||
<td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td> | <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td> | ||
<td style="background-color:#ffe680">Chan, Wei-Jen</td> | <td style="background-color:#ffe680">Chan, Wei-Jen</td> | ||
− | <td style="background-color:#ffe680"> | + | <td style="background-color:#ffe680">1217</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Line 250: | Line 254: | ||
<p><center><font size="2"> | <p><center><font size="2"> | ||
Table 1. the list of our 12 new parts | Table 1. the list of our 12 new parts | ||
− | </font></center></p> | + | </font></center></p><br><br><br> |
<h2> | <h2> | ||
− | + | Validation of composite parts’ function | |
</h2> | </h2> | ||
Line 269: | Line 273: | ||
<p> | <p> | ||
− | <img src="https://static.igem.org/mediawiki/2017/7/71/T--NTHU_Taiwan--Parts--10.png"> | + | <img width="65%" src="https://static.igem.org/mediawiki/2017/7/71/T--NTHU_Taiwan--Parts--10.png"> |
</p> | </p> | ||
Line 275: | Line 279: | ||
<p> | <p> | ||
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it can thus capture our ER-alpha/EDCs complex. | Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it can thus capture our ER-alpha/EDCs complex. | ||
− | </p> | + | </p><br><br> |
Line 285: | Line 289: | ||
<p> | <p> | ||
− | <img src="https://static.igem.org/mediawiki/2017/e/eb/T--NTHU_Taiwan--Parts--k11.png"> | + | <img width="65%" src="https://static.igem.org/mediawiki/2017/e/eb/T--NTHU_Taiwan--Parts--k11.png"> |
</p> | </p> | ||
Line 291: | Line 295: | ||
<p> | <p> | ||
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of <I>E. coli</I> via the function of ice nucleating protein and can bind to EDCs for detection via ER-alpha. | ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of <I>E. coli</I> via the function of ice nucleating protein and can bind to EDCs for detection via ER-alpha. | ||
− | </p> | + | </p><br><br> |
Line 301: | Line 305: | ||
<p> | <p> | ||
− | <img src="https://static.igem.org/mediawiki/2017/0/06/T--NTHU_Taiwan--Parts-k12.png"> | + | <img width="48%" src="https://static.igem.org/mediawiki/2017/0/06/T--NTHU_Taiwan--Parts-k12.png"> |
</p> | </p> | ||
Latest revision as of 16:06, 1 November 2017
Contribution
Interlab measurement study
We have finished the interlab experiments and get lots of results in the page of interlab.
Contribution of new parts
We have contributed 12 new biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Table 1)
biobrick | type | name | description | designer | length |
K2354000 | basic | HRP | Degrade Estrogen Disrupt Chemicals | Chan, Wei-Jen | 927 |
K2354001 | basic | Laccase BP | Degrade Estrogen Disrupt Chemicals | Chan, Wei-Jen | 1572 |
K2354002 | basic | Laccase SC | Degrade Estrogen Disrupt Chemicals | Chan, Wei-Jen | 1539 |
K2354003 | basic | Laccase TC | Degrade Estrogen Disrupt Chemicals | Chan, Wei-Jen | 1593 |
K2354004 | basic | Laccase TV | Degrade Estrogen Disrupt Chemicals | Chan, Wei-Jen | 1557 |
K2354005 | basic | monobody TV | Bind to ER alpha when ER alpha is bind with EDCs | Chan, Wei-Jen | 435 |
K2354006 | basic | MNP TV | Degrade Estrogen Disrupt Chemicals | Chan, Wei-Jen | 1095 |
K2354007 | basic | ER-alpha TV | Bind to EDCs | Chan, Wei-Jen | 759 |
K2354008 | basic | monobody | Bind to ER alpha when ER alpha is bind with EDCs | Chan, Wei-Jen | 288 |
K2354009 | basic | ER-alpha HS | Bind to EDCs | Chan, Wei-Jen | 897 |
K2354010 | composite | monobody-GBP HS | Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface. | Chan, Wei-Jen | 797 |
K2354011 | composite | INP-ER-alpha HS | Expression of ER-alpha on the membrane of E. coli and ER-alpha can bind to EDCs | Chan, Wei-Jen | 2199 |
K2354012 | composite | HRP-his tag | Degrade Estrogen Disrupt Chemicals | Chan, Wei-Jen | 1217 |
Validation of composite parts’ function
We constructed three composite parts and all of their function have been validated:
(1) Part:BBa_K2354010 :monobody-GBP HS
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it can thus capture our ER-alpha/EDCs complex.
(2) Part:BBa_K2354011 :INP-ER-alpha HS
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E. coli via the function of ice nucleating protein and can bind to EDCs for detection via ER-alpha.
(3) Part:BBa_K2354012 :HRP-his tag
Horseradish peroxidase is a kind of peroxidase which uses heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.