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| | <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Applied Design</p> | | <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Applied Design</p> |
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| − | <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Applied Design</p> | + | <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Applied Capsule ingredient statement</p> |
| − | <p>Because the bacteria need to survive in people’s colon, we need to make sure that the bacteria are able reach there without breakdown. Therefore, in our project we designed a capsule with our product in mind. For this aim, the bacteria need to be protected from absorption and injury in the gastrointestinal tract upstream of the colon. Subsequently, the bacteria need to be released immediately. There are various approaches such as designing a capsule coating that can be degraded at above-neutral pH, or that will be degraded after a predetermined time. According to previous studies, some plant polysaccharides such as amylose, inulin, pectin and guar gum are able to withstand the chemical insults of the gastrointestinal tract so that we can deliver the bacteria to the colon more easily. What’s more, because the pH of the gastrointestinal tract is gradually higher, the pH value can also be a control factor in our coating design. Time-dependent drug delivery is also an acceptable method, but there are some drawbacks. Because the time until foods passes through the digestive system and reaches colon is about 6 hours, and this time will change when people eat different foods, people need to control the food type they eat when they are taking our bacteria. Therefore, coating with pH-sensitive polymers is a better choice. We found a previous study that used acrylic resin as main material for pill coatings. The more hydroxy groups there are, the higher the pH value the coating can withstand, because the hydroxy groups will not be dissolved at low pH values. When the pH increases, the polymers are ionized and dissolved. This way, we can achieve successful colonic delivery.</p> | + | <img src="https://static.igem.org/mediawiki/2017/5/57/SHSBNU_17_14a003.jpg"/> |
| | + | <img src="https://static.igem.org/mediawiki/2017/1/1c/SHSBNU_17_13a00.jpg" style="height:100px"/> |
| | + | <img src="https://static.igem.org/mediawiki/2017/d/df/SHSBNU_17_14a001.jpg" style="height:120px"/> |
| | + | <p>(The structure of polyacrylic)</p> |
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| | + | <p>At the inside of capsule, it contains polyacrylic resin to control the dissolving process of the capsule. Polyacrylic is made from Methacrylic acid, Methyl propiolate and acrylic ester in different proportion. Nowadays, polyacrylic resin II and III are the common material used in coating. The effective portion of polyacrylic resin is its carboxyl. When polyacrylic resin in low PH environment, the carboxyl will combine with hydroxyl ion and form salt, so that it is easier for bacteria to pass through. </p> |
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| | + | <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Storage information</p> |
| | + | <p>The probiotic medicinal product contains viable bacteria in a high concentration. For this reason, it should be stored in a refrigerator at a temperature ranging between +2° C and +8° C. An interruption of chilling for a few days will have normally no influence on the number of viable cells. Therefore it is much available for patients to get effective products. </p> |
| | + | <img src="https://static.igem.org/mediawiki/2017/a/aa/SHSBNU_17_14a002.jpg" style="height:250px"/> |
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| | + | <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Safety</p> |
| | + | <p>In case of a prolonged heat exposure, however, the bacterial cells will to some extent lose their viability. No toxic substances will be developed. </p> |
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| | + | <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Sensitivity</p> |
| | + | <p>Because the capsule ingredient has been designed consummately, we can make sure the bacteria will not be released in wrong position, so these bacteria will work intensively. Specific speaking, our engineered bacteria show distinct color-depth difference when exposed to different concentrations of tetrasulfate and thiosulfate, ranging from 0.01 mM to 1mM. The probiotics are able to produce (Flow-process is following), and there will be an obvious result when the chromoprotein mix with excrement for we have down an experiment about color modulation (the result is following)</p> |
| | + | <p><a href="https://static.igem.org/mediawiki/2017/5/53/SHSBNU_17_10a00.jpg"></a></p> |
| | + | <p><a href="https://static.igem.org/mediawiki/2017/b/b3/SHSBNU_17_20b00.jpg"></a></p> |
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| | <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Reference</p> | | <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Reference</p> |
| − | <p>M. K. Chourasia, S. K. Jain, Pharmaceutical approaches to colon targeted drug delivery systems , J Pharm Pharmaceut Sci, 33-66, 2003.</p> | + | <p>杜德才. (2001). 肠溶包衣材料在药物制剂中的应用. <i>中国药业,</i> 10(5), 62-63.</p> |
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