(Prototype team page) |
SaintVansan (Talk | contribs) |
||
(10 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
− | |||
<html> | <html> | ||
+ | <!--[if lt IE 7]> <html class="no-js lt-ie9 lt-ie8 lt-ie7"> <![endif]--> | ||
+ | <!--[if IE 7]> <html class="no-js lt-ie9 lt-ie8"> <![endif]--> | ||
+ | <!--[if IE 8]> <html class="no-js lt-ie9"> <![endif]--> | ||
+ | <!--[if gt IE 8]><!--> <html class="no-js"> <!--<![endif]--> | ||
+ | <head> | ||
+ | <meta charset="utf-8"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <title>TNCR_KOREA — Digestable Gluten</title> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
+ | <meta name="description" content="Free HTML5 Template by FREEHTML5.CO" /> | ||
+ | <meta name="keywords" content="free html5, free template, free bootstrap, html5, css3, mobile first, responsive" /> | ||
+ | <meta name="author" content="FREEHTML5.CO" /> | ||
+ | <meta property="og:title" content=""/> | ||
+ | <meta property="og:image" content=""/> | ||
+ | <meta property="og:url" content=""/> | ||
+ | <meta property="og:site_name" content=""/> | ||
+ | <meta property="og:description" content=""/> | ||
+ | <meta name="twitter:title" content="" /> | ||
+ | <meta name="twitter:image" content="" /> | ||
+ | <meta name="twitter:url" content="" /> | ||
+ | <meta name="twitter:card" content="" /> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | < | + | <link rel="shortcut icon" href="favicon.ico"> |
− | + | ||
− | + | ||
− | </ | + | <link href='https://fonts.googleapis.com/css?family=Source+Sans+Pro:400,700,300' rel='stylesheet' type='text/css'> |
+ | |||
+ | <!-- Animate.css --> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:TNCR_Korea_animate/CSS?action=raw&ctype=text/css"> | ||
+ | <!-- Icomoon Icon Fonts--> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:TNCR_Korea_icomoon/CSS?action=raw&ctype=text/css"> | ||
+ | <!-- Bootstrap --> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:TNCR_Korea_bootstrap/CSS?action=raw&ctype=text/css"> | ||
+ | <!-- Superfish --> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:TNCR_Korea_superfish/CSS?action=raw&ctype=text/css"> | ||
− | < | + | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:TNCR_Korea_style/CSS?action=raw&ctype=text/css"> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | |||
− | < | + | <!-- Modernizr JS --> |
− | < | + | <script src="https://2017.igem.org/Template:TNCR_Korea_modernizr/Javascript? |
− | + | action=raw&ctype=text/javascript"></script> | |
− | + | <!-- FOR IE9 below --> | |
− | < | + | <!--[if lt IE 9]> |
− | + | <script src="https://2017.igem.org/Template:TNCR_Korea_respond/Javascript? | |
− | < | + | action=raw&ctype=text/javascript"></script> |
− | + | <![endif]--> | |
+ | <style> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | /***************************************************** DEFAULT WIKI SETTINGS ****************************************************/ | ||
+ | |||
+ | /* Clear the default wiki settings */ | ||
+ | |||
+ | #home_logo, #sideMenu { display:none; } | ||
+ | #sideMenu, #top_title, .patrollink {display:none;} | ||
+ | #content { width:100%; padding: 0px; margin-top:-7px; margin-left:0px;} | ||
+ | body {background-color:white; } | ||
+ | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; } | ||
+ | |||
+ | |||
+ | |||
+ | #top_menu_under { | ||
+ | position: relative; | ||
+ | width: 100%; | ||
+ | /* height: 18px; */ | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu li{ | ||
+ | display: inline | ||
+ | } | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | #fh5co-menu-wrap { | ||
+ | position: relative; | ||
+ | z-index: 2; | ||
+ | display: block; | ||
+ | float: right; | ||
+ | margin-top: 60px; | ||
+ | } | ||
+ | |||
+ | #fh5co-menu-wrap .sf-menu a { | ||
+ | padding: 1em .75em; | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu > li > ul li.active > a { | ||
+ | color: #FCC72C !important; | ||
+ | } | ||
+ | #fh5co-primary-menu > li > .sf-with-ul:after { | ||
+ | border: none !important; | ||
+ | font-family: 'icomoon'; | ||
+ | speak: none; | ||
+ | font-style: normal; | ||
+ | font-weight: normal; | ||
+ | font-variant: normal; | ||
+ | text-transform: none; | ||
+ | line-height: 1; | ||
+ | /* Better Font Rendering =========== */ | ||
+ | -webkit-font-smoothing: antialiased; | ||
+ | -moz-osx-font-smoothing: grayscale; | ||
+ | position: absolute; | ||
+ | float: right; | ||
+ | margin-right: -5px; | ||
+ | top: 50%; | ||
+ | margin-top: -7px; | ||
+ | content: "\e922"; | ||
+ | color: rgba(255, 255, 255, 0.5); | ||
+ | } | ||
+ | #fh5co-primary-menu > li > ul li { | ||
+ | position: relative; | ||
+ | } | ||
+ | |||
+ | |||
+ | #fh5co-primary-menu > li > ul li > .sf-with-ul:after { | ||
+ | border: none !important; | ||
+ | font-family: 'icomoon'; | ||
+ | speak: none; | ||
+ | font-style: normal; | ||
+ | font-weight: normal; | ||
+ | font-variant: normal; | ||
+ | text-transform: none; | ||
+ | line-height: 1; | ||
+ | /* Better Font Rendering =========== */ | ||
+ | -webkit-font-smoothing: antialiased; | ||
+ | -moz-osx-font-smoothing: grayscale; | ||
+ | position: absolute; | ||
+ | float: right; | ||
+ | margin-right: 10px; | ||
+ | top: 20px; | ||
+ | font-size: 16px; | ||
+ | content: "\e924"; | ||
+ | color: rgba(0, 0, 0, 0.5); | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu { | ||
+ | padding: 10px 7px 10px; | ||
+ | background: #fff; | ||
+ | left: 0; | ||
+ | -webkit-border-radius: 5px; | ||
+ | -moz-border-radius: 5px; | ||
+ | -ms-border-radius: 5px; | ||
+ | border-radius: 5px; | ||
+ | -webkit-box-shadow: 0 5px 15px rgba(0, 0, 0, 0.15); | ||
+ | -moz-box-shadow: 0 5px 15px rgba(0, 0, 0, 0.15); | ||
+ | -ms-box-shadow: 0 5px 15px rgba(0, 0, 0, 0.15); | ||
+ | -o-box-shadow: 0 5px 15px rgba(0, 0, 0, 0.15); | ||
+ | box-shadow: 0 5px 15px rgba(0, 0, 0, 0.15); | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu > li > .fh5co-sub-menu { | ||
+ | left: 100%; | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu:before { | ||
+ | position: absolute; | ||
+ | top: -9px; | ||
+ | left: 20px; | ||
+ | width: 0; | ||
+ | height: 0; | ||
+ | content: ''; | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu:after { | ||
+ | position: absolute; | ||
+ | top: -8px; | ||
+ | left: 21px; | ||
+ | width: 0; | ||
+ | height: 0; | ||
+ | border-right: 8px solid transparent; | ||
+ | border-bottom: 8px solid #fff; | ||
+ | border-bottom: 8px solid white; | ||
+ | border-left: 8px solid transparent; | ||
+ | content: ''; | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu .fh5co-sub-menu:before { | ||
+ | top: 6px; | ||
+ | left: 100%; | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu .fh5co-sub-menu:after { | ||
+ | top: 7px; | ||
+ | right: 100%; | ||
+ | border: none !important; | ||
+ | } | ||
+ | |||
+ | .site-header.has-image #primary-menu .sub-menu { | ||
+ | border-color: #ebebeb; | ||
+ | -webkit-box-shadow: 0 5px 15px 4px rgba(0, 0, 0, 0.09); | ||
+ | -moz-box-shadow: 0 5px 15px 4px rgba(0, 0, 0, 0.09); | ||
+ | -ms-box-shadow: 0 5px 15px 4px rgba(0, 0, 0, 0.09); | ||
+ | -o-box-shadow: 0 5px 15px 4px rgba(0, 0, 0, 0.09); | ||
+ | box-shadow: 0 5px 15px 4px rgba(0, 0, 0, 0.09); | ||
+ | } | ||
+ | .site-header.has-image #primary-menu .sub-menu:before { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu a { | ||
+ | letter-spacing: 0; | ||
+ | padding: 0 15px; | ||
+ | font-size: 18px; | ||
+ | line-height: 34px; | ||
+ | color: #575757 !important; | ||
+ | text-transform: none; | ||
+ | background: none; | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu li { | ||
+ | display: block; | ||
+ | } | ||
+ | |||
+ | #fh5co-primary-menu .fh5co-sub-menu a:hover { | ||
+ | color: #FCC72C !important; | ||
+ | } | ||
+ | |||
+ | #fh5co-work-section, | ||
+ | #fh5co-services-section, | ||
+ | #fh5co-content-section, | ||
+ | #fh5co-work-section_2, | ||
+ | #fh5co-blog-section { | ||
+ | padding: 7em 0; | ||
+ | } | ||
+ | @media screen and (max-width: 768px) { | ||
+ | #fh5co-work-section, | ||
+ | #fh5co-services-section, | ||
+ | #fh5co-content-section, | ||
+ | #fh5co-work-section_2, | ||
+ | #fh5co-blog-section { | ||
+ | padding: 4em 0; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | |||
+ | </style> | ||
+ | |||
+ | |||
+ | <!--- THIS IS WHERE THE HTML BEGINS ---> | ||
+ | |||
+ | </head> | ||
+ | <body> | ||
+ | <div id="fh5co-wrapper"> | ||
+ | <div id="fh5co-page"> | ||
+ | <div id="fh5co-header"> | ||
+ | <header id="fh5co-header-section"> | ||
+ | <div class="container"> | ||
+ | <div class="nav-header"> | ||
+ | <a href="#" class="js-fh5co-nav-toggle fh5co-nav-toggle"><i></i></a> | ||
+ | <h1 id="fh5co-logo"><a href="https://2017.igem.org/Team:TNCR_Korea"><span>TNCR</span>KOREA</a></h1> | ||
+ | <!-- START #fh5co-menu-wrap --> | ||
+ | <nav id="fh5co-menu-wrap" role="navigation"> | ||
+ | <ul class="sf-menu" id="fh5co-primary-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:TNCR_Korea">Home</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="portfolio.html" class="fh5co-sub-ddown">Team</a> | ||
+ | <ul class="fh5co-sub-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Team" target="_blank">Team</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Attributions" target="_blank">Attributions</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Collaborations" target="_blank">Collaborations</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="active"> | ||
+ | <a href="portfolio.html" class="fh5co-sub-ddown">Project</a> | ||
+ | <ul class="fh5co-sub-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Background" target="_blank">Background</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Description" target="_blank">Description</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Design" target="_blank">Design</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Model" target="_blank">Model</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Experiments" target="_blank">Experiments</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Notebook" target="_blank">Notebook</a></li> | ||
+ | |||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Results" target="_blank">Results</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="portfolio.html" class="fh5co-sub-ddown">Parts</a> | ||
+ | <ul class="fh5co-sub-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Parts" target="_blank">Parts</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Contribution" target="_blank">Contribution</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Basic_Part" target="_blank">Basic Part</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Composite_Part" target="_blank">Composite Part</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Part_Collection" target="_blank">Part Collection</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="portfolio.html" class="fh5co-sub-ddown">Human Practices</a> | ||
+ | <ul class="fh5co-sub-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/HP/Silver" target="_blank">Human Activity</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/HP/Gold_Integrated" target="_blank">Integrated Human Activity</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:TNCR_Korea/Engagement" target="_blank">Public Engagement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:TNCR_Korea/Awards">Awards</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | </div> | ||
+ | </div> | ||
+ | </header> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="fh5co-hero fh5co-hero-2"> | ||
+ | <div class="fh5co-overlay"></div> | ||
+ | <div class="fh5co-cover fh5co-cover_2 text-center" data-stellar-background-ratio="0.5" style="background-image: url(images/about-image.jpg);"> | ||
+ | <div class="desc animate-box"> | ||
+ | <h2>Project</h2> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- end:header-top --> | ||
+ | <div id="fh5co-about"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-8 col-md-offset-2 text-center heading-section animate-box"> | ||
+ | <h3>Design</h3> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row"> | ||
+ | <div class="col-md-8 col-md-offset-2 animate-box"> | ||
+ | <h3>To find out the fundamental solution, we casted several questions to think about, focusing on DPP4:</h3> | ||
+ | <ul> | ||
+ | <li>1. What is the difference between gluten-digestion disabled and normal? Is it because of the host’s defect in gene regarding DPP4 or is it because of enterobacteria’s other potential cause of gluten digestion capability?</li> | ||
+ | <li>2. Analyzing the difference of intestinal flora between normal and disabled focusing on DPP4 using</li> | ||
+ | <ul> | ||
+ | <li>NGS (focusing on 16s RNA sequencing)</li> | ||
+ | <li>Terminal RFLP (checking terminal unit’s number between floras)</li> | ||
+ | </ul> | ||
+ | </ul> | ||
+ | <br> | ||
+ | <br> | ||
+ | <h3>As an answer of these questions, we thought of applying the normal enterobacteria’s property of DPP4 into the enterobacteria which is disabled. While brainstorming, we thought of two potential methods:</h3> | ||
+ | <ul> | ||
+ | <li>1. Transmuting the disabled intestinal flora by inserting the normal enterobacteria directly by eating yogurts and checking if the intestinal flora has changed its digesting capability</li> | ||
+ | <li>2. Directly changing the disabled intestinal flora by using synthetic biology: inserting the normal one’s DPP4 gene into the disabled one and improving the digesting capability</li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </br> | ||
+ | </br> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-8 col-md-offset-2 animate-box"> | ||
+ | <p>We chose the second method as it is specifically required in iGEM. We learned about the general process we should be working on by reading and paraphrasing the protocols that are mentioned in the iGEM website. We each made questions about the protocol and asked each other about general process including mini prep, ligation, backbone plasmid, etc.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </br> | ||
+ | </br> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-8 col-md-offset-2 animate-box"> | ||
+ | <p>After we specified the method by several brainstorming, we looked up more about the DPP4 and found out that it accompanies several side-effects if it is in excess amount.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </br> | ||
+ | </br> | ||
+ | </br> | ||
+ | </br> | ||
+ | |||
+ | <div class="row row-bottom-padded-md"> | ||
+ | <div class="col-md-12 animate-box"> | ||
+ | <figure> | ||
+ | <center><img src="https://scontent-icn1-1.xx.fbcdn.net/v/t34.0-12/23140416_537545683249646_922561142_n.png?oh=c5f3eedd615f46a778614bbe427cdd37&oe=59FBD518"img-responsive" width="1000" height="800"></center> | ||
+ | </figure> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row"> | ||
+ | <div class="col-md-8 col-md-offset-2 animate-box"> | ||
+ | <p>Accoding to <i>World Preview 2016, Outlook to 2022</i>'s analysis of Anti-Diabetics Market to 2022, DPP4 inhibitor has been and will be commonly used as a cure of diabetes, which means that overexpression of DPP4 might lead into diabetes. Therefore, we recognized that we had to regulate the expression in order not to cause the overexpression. We thereby knew that additional gene should be used to regulate the expression of DPP4 to reach the best amount of DPP4 of individuals.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </br> | ||
+ | </br> | ||
+ | </br> | ||
+ | </br> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- fh5co-content-section --> | ||
+ | |||
+ | <!-- fh5co-blog-section --> | ||
+ | <footer> | ||
+ | <div id="footer"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6 col-md-offset-3 text-center"> | ||
+ | <p class="fh5co-social-icons"> | ||
+ | <a href="#"><i class="icon-twitter2"></i></a> | ||
+ | <a href="#"><i class="icon-facebook2"></i></a> | ||
+ | <a href="#"><i class="icon-instagram"></i></a> | ||
+ | <a href="#"><i class="icon-dribbble2"></i></a> | ||
+ | <a href="#"><i class="icon-youtube"></i></a> | ||
+ | </p> | ||
+ | <p>Copyright 2016 Free Html5 <a href="#">TNCR_KOREA</a>. All Rights Reserved. <br>Made with <i class="icon-heart3"></i> by <a href="http://freehtml5.co/" target="_blank">Freehtml5.co</a> / Demo Images: <a href="https://unsplash.com/" target="_blank">Unsplash</a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </footer> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | <!-- END fh5co-page --> | ||
+ | |||
+ | </div> | ||
+ | <!-- END fh5co-wrapper --> | ||
+ | |||
+ | <!-- jQuery --> | ||
+ | |||
+ | |||
+ | <script src="https://2017.igem.org/Template:TNCR_Korea_jquery_min/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | <!-- jQuery Easing --> | ||
+ | <script src="https://2017.igem.org/Template:TNCR_Korea_jquery_easing_1_3/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | <!-- Bootstrap --> | ||
+ | <script src="https://2017.igem.org/Template:TNCR_Korea_bootstrap_min/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | <!-- Waypoints --> | ||
+ | <script src="https://2017.igem.org/Template:TNCR_Korea_jquery_waypoints_min/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | <!-- Stellar --> | ||
+ | <script src="https://2017.igem.org/Template:TNCR_Korea_jquery_stellar_min/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | <!-- Superfish --> | ||
+ | <script src="https://2017.igem.org/Template:TNCR_Korea_hoverIntent/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | <script src="https://2017.igem.org/Template:TNCR_Korea_superfish/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | |||
+ | <!-- Main JS (Do not remove) --> | ||
+ | <script src="https://2017.igem.org/Template:TNCR_Korea_main/Javascript? | ||
+ | action=raw&ctype=text/javascript"></script> | ||
+ | |||
+ | </body> | ||
</html> | </html> |
Latest revision as of 16:19, 1 November 2017
Project
Design
To find out the fundamental solution, we casted several questions to think about, focusing on DPP4:
- 1. What is the difference between gluten-digestion disabled and normal? Is it because of the host’s defect in gene regarding DPP4 or is it because of enterobacteria’s other potential cause of gluten digestion capability?
- 2. Analyzing the difference of intestinal flora between normal and disabled focusing on DPP4 using
- NGS (focusing on 16s RNA sequencing)
- Terminal RFLP (checking terminal unit’s number between floras)
As an answer of these questions, we thought of applying the normal enterobacteria’s property of DPP4 into the enterobacteria which is disabled. While brainstorming, we thought of two potential methods:
- 1. Transmuting the disabled intestinal flora by inserting the normal enterobacteria directly by eating yogurts and checking if the intestinal flora has changed its digesting capability
- 2. Directly changing the disabled intestinal flora by using synthetic biology: inserting the normal one’s DPP4 gene into the disabled one and improving the digesting capability
We chose the second method as it is specifically required in iGEM. We learned about the general process we should be working on by reading and paraphrasing the protocols that are mentioned in the iGEM website. We each made questions about the protocol and asked each other about general process including mini prep, ligation, backbone plasmid, etc.
After we specified the method by several brainstorming, we looked up more about the DPP4 and found out that it accompanies several side-effects if it is in excess amount.
![](https://scontent-icn1-1.xx.fbcdn.net/v/t34.0-12/23140416_537545683249646_922561142_n.png?oh=c5f3eedd615f46a778614bbe427cdd37&oe=59FBD518)
Accoding to World Preview 2016, Outlook to 2022's analysis of Anti-Diabetics Market to 2022, DPP4 inhibitor has been and will be commonly used as a cure of diabetes, which means that overexpression of DPP4 might lead into diabetes. Therefore, we recognized that we had to regulate the expression in order not to cause the overexpression. We thereby knew that additional gene should be used to regulate the expression of DPP4 to reach the best amount of DPP4 of individuals.