Difference between revisions of "Team:UCLouvain/Composite Part"

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<h3>★  ALERT! </h3>
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<div class="big-title montserrat-text uppercase">in the lab</div>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<div class="small-title montserrat-text uppercase"><br><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">Composite Parts</Marine></div>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<span>New parts</span>
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<p class="montserrat-text uppercase">pCMMCD 105</p>
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                        <p>Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.</p>
  
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                        <p>GusA encodes the <i>beta</i>-glucuronidase gene from <i>E. coli</i>. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and <i>para</i>-nitrophenyl glucuronide.</p>
  
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<p>Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).</p>
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<h1>Composite Parts</h1>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<span>iGEM parts</span>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<p class="montserrat-text uppercase">piFinal <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_K577882">(BBa_K577882)</a></p>
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                        <p>iGEM plasmid consisting of araC, a pBAD/AraC promoter (I0500), an RBS (B0034), an RFP (E1010), and two terminators (B0010 and B0012) contained inside one biobrick (B0015)</p>
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<h3>Best Composite Part Special Prize</h3>
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
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<h4>Note</h4>
 
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
 
  
 
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Latest revision as of 16:55, 1 November 2017

iGEM UCLouvain Team iGEM UCLouvain Team

in the lab

Composite Parts
New parts


pCMMCD 105

Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.

GusA encodes the beta-glucuronidase gene from E. coli. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and para-nitrophenyl glucuronide.

Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).



iGEM parts


piFinal (BBa_K577882)

iGEM plasmid consisting of araC, a pBAD/AraC promoter (I0500), an RBS (B0034), an RFP (E1010), and two terminators (B0010 and B0012) contained inside one biobrick (B0015)

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