Difference between revisions of "Team:SHSBNU China/Improve"

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    <title>SHSBNU-China iGEM 2017 Home Page</title>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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    <div style="overflow: hidden; z-index: -1; position: fixed; height: 100%; width: 100%; top: 0px; left: 0px; ">
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        <img  id="background_picture" src="https://static.igem.org/mediawiki/2017/e/ee/SHSBNU_17_background.jpg" style="position: fixed; display: block; left: 0px; top: 0px;">
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            <a href="https://2017.igem.org/Team:SHSBNU_China"> <img src="https://static.igem.org/mediawiki/2017/a/aa/SHSBNU_17_Banner.jpg" /></a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Project" style = "opacity: 1;">Project</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Model" style = "opacity: 1;">Model</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/HP" style = "opacity: 1;">HP</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Safety" style = "opacity: 1;">Safety</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Parts" style = "opacity: 1;">Parts</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Team" style = "opacity: 1;">Team</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Achievement" style = "opacity: 1;">Achievement</a>
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            <p style = "font-family:arial;color:#444;font-size:30px; text-align: center">Project</p>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Project" style = "opacity: 1;">Project</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Experiment" style = "opacity: 1;">Experiment</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Improve" style = "opacity: 1;">Improve</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Demonstrate" style = "opacity: 1;">Demonstrate</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Applied_Design" style = "opacity: 1;">Applied Design</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Protocol" style = "opacity: 1;">Protocol</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/InterLab" style = "opacity: 1;">InterLab</a>
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        <div class="post-75 post type-post status-publish format-standard hentry category-azir-quantum-science concavebox">
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            <div class="entry" style="opacity: 0.95;">
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                <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Improvement</p>
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                <p>Before our marine <i>Shewanella baltica</i> TtrS/R (<a href="http://parts.igem.org/Part:BBa_K2507013" style = "opacity: 1;">BBa_K2507013</a>,  <a href="http://parts.igem.org/Part:BBa_K2507014" style = "opacity: 1;">BBa_K2507014</a>, <a href="http://parts.igem.org/Part:BBa_K2507015" style = "opacity: 1;">BBa_K2507015</a>, <a href="http://parts.igem.org/Part:BBa_K2507016" style = "opacity: 1;">BBa_K2507016</a>, <a href="http://parts.igem.org/Part:BBa_K2507017" style = "opacity: 1;">BBa_K2507017</a>) system, the only known genetically encoded tetrathionate sensor is the <i>Salmonella typhimurium</i> TtrSR two-component system (TCS) (Hensel et al, 1999; Price-Carter et al, 2001). However, they found <i>S.typhimurium’s</i> promoter PttrB is repressed by oxygen and nitrate via the global regulator Nitrate Reductase Regulator (FNR). While the oxygen levels in the intestinal are poorly understood by human beings Kristina et al think the oxygen level maybe relatively high near the epithelial mucosal boundary because of near to the blood. Meanwhile, nitrate levels in intestinal have been shown to be improved during inflammation (Winter et al, 2013). In iGEM 2012, Dundee used <i>Salmonella</i> TtrS/R system  <a href="http://parts.igem.org/Part:BBa_K895007" style = "opacity: 1;">BBa_K895007</a> and the system works well.</p>
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                <P>The sensor we use <i>S.baltica</i> TtrS/R which is only weakly repressed by oxygen and not repressed by nitrate in <i>E. coli.</i> And our <strong><i>S.baltica</i> TtrS/R system can work well using protoviolaceinic acid as the reporter</strong><a href="http://parts.igem.org/Part:BBa_K2507017" style = "opacity: 1;">BBa_K2507017</a>, which display dark-green color. And our system could also work poorly using sfGFP as the reporter.</P>
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                <img src="https://static.igem.org/mediawiki/2017/7/79/SHSBNU_17_12a00.jpg" style=""/>
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                <p>Figure1. Schematic diagram of ligand-induced signaling through TtrS/R and plasmid-borne implementation of the sensor components. We combine BBa_ <a href="http://parts.igem.org/Part:BBa_K2507006" style = "opacity: 1;">BBa_K2507006</a>with <a href="http://parts.igem.org/Part:BBa_K2507017" style = "opacity: 1;">BBa_K2507017</a>.</p>
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                <img src="https://static.igem.org/mediawiki/2017/0/0e/SHSBNU_17_11d06.jpg" style="height:200px"/>
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                <p>Figure2. Overnight cultures of E. coli Top10 overexpressing vioABDE, producing protoviolaceinic acid in different tetrathionate concentration. </p>
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                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> References</p>
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                <p>Hensel M, Hinsley AP, Nikolaus T, Sawers G, Berks BC (1999) The genetic basis of tetrathionate respiration in Salmonella typhimurium. <i>Mol Microbiol</i> 32: 275 – 287 </p>
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                <p>Price-Carter M, Tingey J, Bobik TA, Roth JR (2001) The alternative electron acceptor tetrathionate supports B12-dependent anaerobic growth of Salmonella enterica serovar typhimurium on ethanolamine or 1,2- propanediol. <i>J Bacteriol </i> 183: 2463 – 2475 </p>
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                  <p>Winter SE, Winter MG, Xavier MN, Thiennimitr P, Poon V, Keestra AM, Laughlin RC, Gomez G, Wu J, Lawhon SD, Popova IE, Parikh SJ, Adams LG, Tsolis RM, Stewart VJ, Baumler AJ (2013) Host-derived nitrate boosts growth of E. coli in the inflamed gut. <i>Science </i> 339: 708 – 711 </p>
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                <a href="http://www.shsbnu.net" style = "float:left"><img src = "https://static.igem.org/mediawiki/2017/4/44/SHSBNU_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
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                <a href="http://www.azirquantum.net.cn" style = "float:left"><img src = "https://static.igem.org/mediawiki/2017/1/1f/Azir_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
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                <a href="https://2017.igem.org" style = "float:right"><img src = "https://static.igem.org/mediawiki/2017/6/60/IGEM_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
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                <a href="http://www.bluepha.com" style = "float:right"><img src = "https://static.igem.org/mediawiki/2017/b/bc/Bluepha_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
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                <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">iGEM 2017</P>
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                <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">Made by Azir.</P>
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                <p style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">The Second High School Attached Beijing Normal University International Division </p>
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Latest revision as of 17:01, 1 November 2017

SHSBNU-China iGEM 2017 Home Page

Improvement

Before our marine Shewanella baltica TtrS/R (BBa_K2507013, BBa_K2507014, BBa_K2507015, BBa_K2507016, BBa_K2507017) system, the only known genetically encoded tetrathionate sensor is the Salmonella typhimurium TtrSR two-component system (TCS) (Hensel et al, 1999; Price-Carter et al, 2001). However, they found S.typhimurium’s promoter PttrB is repressed by oxygen and nitrate via the global regulator Nitrate Reductase Regulator (FNR). While the oxygen levels in the intestinal are poorly understood by human beings Kristina et al think the oxygen level maybe relatively high near the epithelial mucosal boundary because of near to the blood. Meanwhile, nitrate levels in intestinal have been shown to be improved during inflammation (Winter et al, 2013). In iGEM 2012, Dundee used Salmonella TtrS/R system BBa_K895007 and the system works well.

The sensor we use S.baltica TtrS/R which is only weakly repressed by oxygen and not repressed by nitrate in E. coli. And our S.baltica TtrS/R system can work well using protoviolaceinic acid as the reporterBBa_K2507017, which display dark-green color. And our system could also work poorly using sfGFP as the reporter.

Figure1. Schematic diagram of ligand-induced signaling through TtrS/R and plasmid-borne implementation of the sensor components. We combine BBa_ BBa_K2507006with BBa_K2507017.

Figure2. Overnight cultures of E. coli Top10 overexpressing vioABDE, producing protoviolaceinic acid in different tetrathionate concentration.

References

Hensel M, Hinsley AP, Nikolaus T, Sawers G, Berks BC (1999) The genetic basis of tetrathionate respiration in Salmonella typhimurium. Mol Microbiol 32: 275 – 287

Price-Carter M, Tingey J, Bobik TA, Roth JR (2001) The alternative electron acceptor tetrathionate supports B12-dependent anaerobic growth of Salmonella enterica serovar typhimurium on ethanolamine or 1,2- propanediol. J Bacteriol 183: 2463 – 2475

Winter SE, Winter MG, Xavier MN, Thiennimitr P, Poon V, Keestra AM, Laughlin RC, Gomez G, Wu J, Lawhon SD, Popova IE, Parikh SJ, Adams LG, Tsolis RM, Stewart VJ, Baumler AJ (2013) Host-derived nitrate boosts growth of E. coli in the inflamed gut. Science 339: 708 – 711

iGEM 2017

Made by Azir.

The Second High School Attached Beijing Normal University International Division