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new biochemical reactions is an important research direction of synthetic biology. | new biochemical reactions is an important research direction of synthetic biology. | ||
<br><br> ceaS2, whose full name is N2-(2-carboxyethyl)arginine synthase2, is a kind of enzyme in Streptomyces | <br><br> ceaS2, whose full name is N2-(2-carboxyethyl)arginine synthase2, is a kind of enzyme in Streptomyces | ||
− | clavuligerus. The mentor of our team, Jiang Huifeng, has confirmed the new functions of ceaS2 with the help of TPP (Thiamine pyrophosphate) and magnesium ions | + | clavuligerus. The mentor of our team, Jiang Huifeng, has confirmed the new functions of ceaS2 with the help of TPP (Thiamine pyrophosphate) and magnesium ions. ceaS2 enzyme can catalyze the production of acrylic acid with DHAP (dihydroxy acetone phosphate) or G3P (glyceraldehyde 3-phosphate) as substrate. |
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<br><br> Cell factory of acrylic acid (GAACF) 1.0: | <br><br> Cell factory of acrylic acid (GAACF) 1.0: | ||
<br><br> DHAP and G3P are the central metabolic secondary products which can be easily found in various organisms. | <br><br> DHAP and G3P are the central metabolic secondary products which can be easily found in various organisms. | ||
They are the carbon flow nodes that must be passed in the glycerol metabolic pathway in most organisms. | They are the carbon flow nodes that must be passed in the glycerol metabolic pathway in most organisms. | ||
− | + | ceaS2 enzyme being the core part, it is possible to create a new pathway to synthesize acrylic acid based on glycerol metabolic pathway in organisms and construct a cell factory with a high yield of acrylic acid. | |
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<br><br> First, we took E. coli BL21 (DE3) as the chassis cells and constructed engineering bacteria carrying | <br><br> First, we took E. coli BL21 (DE3) as the chassis cells and constructed engineering bacteria carrying | ||
the gene of ceaS2 enzyme with pET-28a plasmid as the vector. We constructed a new pathway to synthesize | the gene of ceaS2 enzyme with pET-28a plasmid as the vector. We constructed a new pathway to synthesize |
Revision as of 17:06, 1 November 2017