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yield multiple is a new indicator, the result is as follows: | yield multiple is a new indicator, the result is as follows: | ||
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− | <img src="https://static.igem.org/mediawiki/2017/2/2a/Sxt_%281%29.png" style="max-width:60%;"> | + | <img src="https://static.igem.org/mediawiki/2017/2/2a/Sxt_%281%29.png" style="max-width:60%;"><br /> |
The horizontal axis in the figure is the position of each mutational site, and the | The horizontal axis in the figure is the position of each mutational site, and the | ||
vertical axis is the multiple of the acrylic acid yield of each mutational site compared | vertical axis is the multiple of the acrylic acid yield of each mutational site compared | ||
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Fig3. A comparison of OD of BY4741 and MG1655 under 500mg/L acrylic acid | Fig3. A comparison of OD of BY4741 and MG1655 under 500mg/L acrylic acid | ||
<br /> | <br /> | ||
− | <img src="https://static.igem.org/mediawiki/2017/2/2a/Sxt_%281%29.png" style="max-width:60%;"> | + | <img src="https://static.igem.org/mediawiki/2017/2/2a/Sxt_%281%29.png" style="max-width:60%;"><br /> |
Fig4. A comparison of OD of BY4741 and MG1655 under 1000mg/L acrylic acid | Fig4. A comparison of OD of BY4741 and MG1655 under 1000mg/L acrylic acid | ||
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For E. coli, yields of using new and old synthetic pathways of acrylic acid are as follows: | For E. coli, yields of using new and old synthetic pathways of acrylic acid are as follows: | ||
Conditions: reaction time 42h, PH8.0, glycerol concentration 1% | Conditions: reaction time 42h, PH8.0, glycerol concentration 1% | ||
− | <img src="https://static.igem.org/mediawiki/2017/2/2a/Sxt_%281%29.png" style="max-width:60%;"> | + | <img src="https://static.igem.org/mediawiki/2017/2/2a/Sxt_%281%29.png" style="max-width:60%;"><br /> |
It can be seen that the acrylic acid yield is increased by 3 times after the introduction | It can be seen that the acrylic acid yield is increased by 3 times after the introduction | ||
of the GlyDH enzyme and the DAK enzyme compared to the introduction of only | of the GlyDH enzyme and the DAK enzyme compared to the introduction of only |
Revision as of 17:30, 1 November 2017