(23 intermediate revisions by 4 users not shown) | |||
Line 7: | Line 7: | ||
<title>Interlab</title> | <title>Interlab</title> | ||
<link rel="stylesheet" href="https://2017.igem.org/Team:HUST-China/css/bootstrap?action=raw&ctype=text/css"> | <link rel="stylesheet" href="https://2017.igem.org/Team:HUST-China/css/bootstrap?action=raw&ctype=text/css"> | ||
− | + | <link href="https://2017.igem.org/Team:HUST-China/css/return_top?action=raw&ctype=text/css" rel="stylesheet" type="text/css" /> | |
− | + | <link rel="stylesheet" href="https://2017.igem.org/Team:HUST-China/css/picbox.css?action=raw&ctype=text/css" type="text/css" media="screen" /> | |
+ | |||
<script> | <script> | ||
if ($('.firstHeading')) $('.firstHeading').remove(); | if ($('.firstHeading')) $('.firstHeading').remove(); | ||
Line 299: | Line 300: | ||
ul.nav-pills2 { | ul.nav-pills2 { | ||
bottom:50px; | bottom:50px; | ||
− | |||
position: fixed; | position: fixed; | ||
} | } | ||
Line 309: | Line 309: | ||
} | } | ||
− | @media screen and (max-width: | + | @media screen and (max-width: 992px) { |
#section1, #section2, #section3, #section4, #section5 { | #section1, #section2, #section3, #section4, #section5 { | ||
margin-left: 50px; background-color: #eee; color:#171717; | margin-left: 50px; background-color: #eee; color:#171717; | ||
} | } | ||
+ | } | ||
+ | .yuandian li {list-style-type:circle; } | ||
+ | |||
+ | body { | ||
+ | position: relative; | ||
+ | } | ||
+ | @media (min-width: 768px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:10px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:100px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:8px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 中等屏幕(桌面显示器,大于等于992px) */ | ||
+ | @media (min-width: 992px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:20px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:110px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:10px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 大屏幕(大桌面显示器,大于等于1200px) */ | ||
+ | @media (min-width: 1200px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:80px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:170px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:12px; | ||
+ | font-weight: bold; | ||
+ | } | ||
} | } | ||
Line 319: | Line 382: | ||
<body data-spy="scroll" data-target="#myScrollspy" data-offset="20"> | <body data-spy="scroll" data-target="#myScrollspy" data-offset="20"> | ||
− | + | <nav class="navbar navbar-default navbar-fixed-top" style="margin-top:15px"> | |
<div class="container"> | <div class="container"> | ||
− | |||
<div class="navbar-header"> | <div class="navbar-header"> | ||
− | <a href="https://2017.igem.org/Team:HUST-China" class="navbar-brand logo"><img src="https://static.igem.org/mediawiki/2017/6/64/Team_HUST_China_2017_logo.png" alt="HUST_iGEM_2017_logo"></a> | + | <a href="https://2017.igem.org/Team:HUST-China" class="navbar-brand logo menu_logo_1"><img src="https://static.igem.org/mediawiki/2017/6/64/Team_HUST_China_2017_logo.png" alt="HUST_iGEM_2017_logo"></a> |
− | <a href="https://2017.igem.org" class="navbar-brand logo"><img src="https://static.igem.org/mediawiki/2017/f/f6/IGEM_2017_logo.png" alt="HUST_iGEM_2017_logo"></a> | + | <a href="https://2017.igem.org" class="navbar-brand logo menu_logo_2"><img src="https://static.igem.org/mediawiki/2017/f/f6/IGEM_2017_logo.png" alt="HUST_iGEM_2017_logo"></a> |
− | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#navbar-collapse"> | + | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#navbar-collapse" style="background-color: #333; color:#111"> |
− | <span class=" | + | <span class="icon-bar"></span> |
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
</button> | </button> | ||
</div> | </div> | ||
Line 333: | Line 398: | ||
<div class="collapse navbar-collapse" id="navbar-collapse"> | <div class="collapse navbar-collapse" id="navbar-collapse"> | ||
<ul class="nav navbar-nav navbar-right"> | <ul class="nav navbar-nav navbar-right"> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China">HOME</a></li> | ||
+ | <li class="dropdown active"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
+ | PROJECT <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/project/background">Background</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Description">Description</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Safety">Safety</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Demonstrate">Demonstrate</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
+ | WETLAB <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class<nav class="navbar navbar-default navbar-fixed-top" style="margin-top:15px"> | ||
+ | <div class="container"> | ||
+ | <div class="navbar-header"> | ||
+ | <a href="https://2017.igem.org/Team:HUST-China" class="navbar-brand logo menu_logo_1"><img src="https://static.igem.org/mediawiki/2017/6/64/Team_HUST_China_2017_logo.png" alt="HUST_iGEM_2017_logo"></a> | ||
+ | <a href="https://2017.igem.org" class="navbar-brand logo menu_logo_2"><img src="https://static.igem.org/mediawiki/2017/f/f6/IGEM_2017_logo.png" alt="HUST_iGEM_2017_logo"></a> | ||
+ | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#navbar-collapse" style="background-color: #333; color:#111"> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | </button> | ||
+ | </div> | ||
− | + | <div class="collapse navbar-collapse" id="navbar-collapse"> | |
+ | <ul class="nav navbar-nav navbar-right"> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China">HOME</a></li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown"> | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
Line 343: | Line 438: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2017.igem.org/Team:HUST-China/project/background">Background</a></li> | <li><a href="https://2017.igem.org/Team:HUST-China/project/background">Background</a></li> | ||
− | <li><a href="https://2017.igem.org/Team:HUST-China/Description">Description</a></li> | + | <li><a href="https://2017.igem.org/Team:HUST-China/Description">Description</a></li> |
<li><a href="https://2017.igem.org/Team:HUST-China/Safety">Safety</a></li> | <li><a href="https://2017.igem.org/Team:HUST-China/Safety">Safety</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Demonstrate">Demonstrate</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
Line 360: | Line 456: | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
− | <a href="https://2017.igem.org/Team:HUST-China/ | + | <a href="https://2017.igem.org/Team:HUST-China/Model" > |
− | + | MODEL | |
</a> | </a> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
− | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | |
− | PARTS | + | PARTS<b class="caret"></b> |
</a> | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Basic part">Basic part</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Composite part">Composite part</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Part collection">Part collection</a></li> | ||
+ | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
− | <a href=" | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> |
− | + | HP<b class="caret"></b> | |
</a> | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Collaborations"> Collaborations</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/HP/Silver">HP/Silver</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Engagement">Engagement</a></li> | ||
+ | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
Line 383: | Line 489: | ||
</ul> | </ul> | ||
</li> | </li> | ||
+ | |||
+ | |||
</ul> | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
</nav> | </nav> | ||
− | |||
<div class="tab1" id="section0" style="width:100%;margin-top: 65px;"> | <div class="tab1" id="section0" style="width:100%;margin-top: 65px;"> | ||
Line 404: | Line 511: | ||
<li><a href="#section4">Instrument</a></li> | <li><a href="#section4">Instrument</a></li> | ||
<li><a href="#section5">Calibration Protocol</a></li> | <li><a href="#section5">Calibration Protocol</a></li> | ||
+ | <li><a href="#section6">Cell Culture</a></li> | ||
+ | <li><a href="#section7">Interlab Results</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Line 417: | Line 526: | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Provenance and Release</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Provenance and Release</h3> | ||
<h4><strong>①Individuals responsible for conducting InterLab study</strong> </h4> | <h4><strong>①Individuals responsible for conducting InterLab study</strong> </h4> | ||
− | <div class="table-responsive" style="padding: 10px 100px; text-align: center;"> | + | <div class="table-responsive" style="padding: 10px 100px; text-align: center;font-size:14px;"> |
− | <table class="table | + | <table class="table table-hover"> |
<thead> | <thead> | ||
<tr> | <tr> | ||
Line 442: | Line 551: | ||
</div> | </div> | ||
<h4><strong> ②Corresponding email</strong> </h4> | <h4><strong> ②Corresponding email</strong> </h4> | ||
− | <div class="table-responsive" style="padding: 10px 100px; text-align: center;"> | + | <div class="table-responsive" style="padding: 10px 100px; text-align: center;font-size:14px;"> |
− | <table class="table | + | <table class="table table-hover"> |
<thead> | <thead> | ||
<tr> | <tr> | ||
Line 457: | Line 566: | ||
<tr> | <tr> | ||
<td>Kangyuan Yu</td> | <td>Kangyuan Yu</td> | ||
− | <td> | + | <td>u201612160@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Haibo Huang </td> | <td>Haibo Huang </td> | ||
− | <td> | + | <td>u201612177@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Ziyang Xiao</td> | <td>Ziyang Xiao</td> | ||
− | <td> | + | <td>u201612166@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Shaofeng Liao</td> | <td>Shaofeng Liao</td> | ||
− | <td> | + | <td>u201612159@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>HuiPing Shi</td> | <td>HuiPing Shi</td> | ||
− | <td> | + | <td>u201513154@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 486: | Line 595: | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | ||
<h4><strong> What chassis did you use?</strong> </h4> | <h4><strong> What chassis did you use?</strong> </h4> | ||
− | <p>Escherichia coli | + | <p>Escherichia coli DH5α</p> |
<h4><strong> What Biosafety Level is your chassis? </strong> </h4> | <h4><strong> What Biosafety Level is your chassis? </strong> </h4> | ||
<p>BSL1</p> | <p>BSL1</p> | ||
Line 529: | Line 638: | ||
<li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | <li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | ||
<li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> | <li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
Line 567: | Line 675: | ||
<li>③ Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li> | <li>③ Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li> | ||
<li>④ *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li> | <li>④ *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
+ | <h5>Measurement work flow:</h5> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/f/f8/2017_HUST_China_interlab_img_001.png" rel="lightbox-demo" title="my caption"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/f/f8/2017_HUST_China_interlab_img_001.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | <div class="col-xs-12" > | ||
+ | <h5><strong> Part 2: Prepare the serial dilutions of Fluorescein </strong> </h5> | ||
+ | <ul style="list-style: none; font-size:10px;"> | ||
+ | <li>①Add 100 μl of 1xPBS into wells A2, B2, C2, D2....A12, B12, C12, D12.</li> | ||
+ | <li>②Add 200 μl of 1x Fluorescein stock solution into A1, B1, C1, D1.</li> | ||
+ | <li>③ Transfer 100 μl of Fluorescein stock solution from A1 into A2.</li> | ||
+ | <li>④ Mix A2 by pipetting up and down 3x and transfer 100 μl into A3.</li> | ||
+ | <li>⑤ Mix A3 by pipetting up and down 3x and transfer 100 μl into A4.</li> | ||
+ | <li>⑥ Mix A4 by pipetting up and down 3x and transfer 100 μl into A5.</li> | ||
+ | <li>⑦ Mix A5 by pipetting up and down 3x and transfer 100 μl into A6.</li> | ||
+ | <li>⑧ Mix A6 by pipetting up and down 3x and transfer 100 μl into A7.</li> | ||
+ | <li>⑨ Mix A7 by pipetting up and down 3x and transfer 100 μl into A8.</li> | ||
+ | <li>⑩ Mix A8 by pipetting up and down 3x and transfer 100 μl into A9.</li> | ||
+ | <li>⑪ Mix A9 by pipetting up and down 3x and transfer 100 μl into A10.</li> | ||
+ | <li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | ||
+ | <li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | ||
+ | <li>⑭ Take care not to continue serial dilution into column 12.</li> | ||
+ | <li>⑮ Repeat dilution series for rows B, C, D. | ||
+ | <li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | ||
+ | </ul> | ||
+ | |||
+ | </div> | ||
</div> | </div> | ||
+ | <div id="section6" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
+ | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Cell Culture Setup and Measurement</h3> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/e/e6/2017_HUST_China_interlab_img_002.png" rel="lightbox-demo" title="my caption"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/e/e6/2017_HUST_China_interlab_img_002.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | <div class="col-xs-12"> | ||
+ | <h5><strong>Transformation:</strong> </h5> | ||
+ | <ul class="yuandian"> | ||
+ | <li> control</li> | ||
+ | <li> Negative control</li> | ||
+ | <li>Test Device 1: J23101+I13504</li> | ||
+ | <li>Test Device 2: J23106+I13504</li> | ||
+ | <li>Test Device 3: J23117+I13504</li> | ||
+ | <li>Test Device 4: J23101.BCD2.E0040.B0015</li> | ||
+ | <li>Test Device 5: J23106.BCD2.E0040.B0015</li> | ||
+ | <li>Test Device 6: J23117.BCD2.E0040.B0015</li> | ||
+ | </ul> | ||
+ | <h5><strong>Cell growth:</strong> </h5> | ||
+ | <li class="dropdown" style="list-style-type:none;"> | ||
+ | <button href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
+ | More details <b class="caret"></b> | ||
+ | </button> | ||
+ | <ul class="dropdown-menu" style="padding: 10px; background:#ccc;"> | ||
+ | |||
+ | <li> ① Pick 2 colonies from each of plate and inoculate each in 5-10 mL LB medium + Chloramphenicol (For antibiotic concentrations, please follow these guidelines:<a href="http://parts.igem.org/Help:Protocols/Antibiotic_Stocks">http://parts.igem.org/Help:Protocols/Antibiotic_Stocks). | ||
+ | </a> | ||
+ | </li> | ||
+ | <li>② Grow the cells overnight (16-18 hours) at 37°C and 220 rpm.</li> | ||
+ | <li>③ With 8 total devices (including controls) and 2 colonies per device, you should have 16 cultures.</li> | ||
+ | |||
+ | </ul> | ||
+ | </li> | ||
+ | <h5><strong>Cell growth, sampling, and assay</strong> </h5> | ||
+ | <li class="dropdown" style="list-style-type:none;"> | ||
+ | <button href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
+ | More details <b class="caret"></b> | ||
+ | </button> | ||
+ | <ul class="dropdown-menu" style="padding: 10px; background:#ccc;"> | ||
+ | |||
+ | <li>① Set your instrument to read Abs600 (as OD calibration setting)</li> | ||
+ | <li>② Measure Abs600 of the overnight cultures</li> | ||
+ | <li>③ Dilute the cultures to a target Abs600 of 0.02 (see the volume of preloading culture and media in Excel Dilution Calculation sheets) in 12 ml LB medium + Chloramphenicol in 50 mL falcon tube.</li> | ||
+ | <li>④ Remove 500uL of each culture for your zero time point and hold these samples on ice. (You should have 16 sample tubes from the time=0 time point)</li> | ||
+ | <li>⑤ Incubate the cultures at 37°C and 220 rpm.</li> | ||
+ | <li>⑥ Remove 500uL samples of each culture after 2, 4 and 6 hours of growth. Keep samples on ice while completing the additional time points. You should have 16 sample tubes for each time point.</li> | ||
+ | <li>⑦ Set up your four measurement plates: For colony #1 culture, pipet 100 uL samples into wells A, B, C and D of the row for that device. For colony #2, pipet 100uL samples into wells E, F, G, and H of the row for that device.</li> | ||
+ | <li>⑧ Read your plates, taking care to use the exact same settings used for your fluorescein measurement.</li> | ||
+ | |||
+ | |||
+ | </ul> | ||
+ | </li> | ||
+ | <h5><strong>The initial OD600 measurement of our overnight cultures.</strong> </h5> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/1/1a/2017_HUST_China_interlab_img_table1.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </div> | ||
+ | <div class="col-xs-12"> | ||
+ | <h5><strong>What type of media did you use for this step? </strong> </h5> | ||
+ | <p>Luria Bertani </p> | ||
+ | <h5><strong>What type of vessel or container did you use to grow your cells? </strong> </h5> | ||
+ | <p>50 ml Falcon tube</p> | ||
+ | <h5><strong>What temperature setting did you use during the measurement?</strong> </h5> | ||
+ | <p>22℃</p> | ||
+ | <h5><strong>What type of 96-well plates did you use?</strong> </h5> | ||
+ | <p></p> | ||
+ | <h5><strong>Black plates with transparent/clear bottom (preferred)</strong> </h5> | ||
+ | <p>Flat</p> | ||
+ | <h5><strong>Measurement </strong> </h5> | ||
+ | <ul class="yuandian"> | ||
+ | <li>Measure OD and fluorescence of all samples</li> | ||
+ | </ul> | ||
+ | <h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/8/87/2017_HUST_China_interlab_img_003.png" rel="lightbox-demo" title="my caption"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/8/87/2017_HUST_China_interlab_img_003.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div id="section7" style="border: solid 1px #666; margin:5px; padding:5px;border-radius:10px;overflow: hidden;"> | ||
+ | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Interlab Resultn</h3> | ||
+ | <h5 class="col-xs-12"><strong>OD600 Reference Point</strong> </h5> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/5/52/2017_HUST_China_interlab_img_table2.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | <h5 class="col-xs-12"><strong>Fluorescence standard curve</strong> </h5> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/a/a1/2017_HUST_China_interlab_img_table3.png" rel="lightbox-demo" title="my caption"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/a/a1/2017_HUST_China_interlab_img_table3.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | <p class="col-xs-12"><strong>NOTE: 50uM Sample exceeds the range of measurements</strong> </p> | ||
+ | <h5 class="col-xs-12"><strong>OD600 Reference Point</strong> </h5> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/d/d1/2017_HUST_China_interlab_img_table4.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/d/df/2017_HUST_China_interlab_img_table5.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/b/b3/2017_HUST_China_interlab_img_table6.png" rel="lightbox-demo" title="my caption"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b3/2017_HUST_China_interlab_img_table6.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | <p class="col-xs-12"><strong>Final scaling level determined from medium-high points likely to be less impacted by saturation or pipetting error.</strong> </p> | ||
+ | <p class="col-xs-12"><strong>If needed, you can shift which points are used, but it is likely better to correct instrument settings and protocol.</strong> </p> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/b/b8/2017_HUST_China_interlab_img_table7.png" rel="lightbox-demo" title="my caption"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b8/2017_HUST_China_interlab_img_table7.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/e/ea/2017_HUST_China_interlab_img_table8.png" title="my caption"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/e/ea/2017_HUST_China_interlab_img_table8.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/d/d2/2017_HUST_China_interlab_img_table9.png" rel="lightbox-demo" title="my caption"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/d/d2/2017_HUST_China_interlab_img_table9.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | <p><strong><span class="col-xs-12">Click on the picture and you can see a clearer picture.Then click on the return key of the browser and you can return.</span></strong></p><br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/2/2b/2017_HUST_China_interlab_img_table10.png" rel="lightbox-demo" title="my caption" style="padding: 10px;"><br /> | ||
+ | <img title="demo1" src="https://static.igem.org/mediawiki/2017/2/2b/2017_HUST_China_interlab_img_table10.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | ||
+ | </a> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
</div> | </div> | ||
+ | |||
+ | |||
<nav class="col-sm-2" id="myScrollspy2.0"> | <nav class="col-sm-2" id="myScrollspy2.0"> | ||
<div class="container-fluid"> | <div class="container-fluid"> | ||
<div class="collapse navbar-collapse" id="navbar-collapse"> | <div class="collapse navbar-collapse" id="navbar-collapse"> | ||
− | <ul class="nav nav-pills2 nav-stacked | + | <ul class="nav nav-pills2 nav-stacked"> |
− | <li class="active">< | + | <li class="active"> |
+ | <div style="display:none;" id="rocket-to-top"> | ||
+ | <div style="opacity:0;display:block;" class="level-2"></div> | ||
+ | <div class="level-3"></div> | ||
+ | </div> | ||
+ | </li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Line 585: | Line 843: | ||
</div> | </div> | ||
</div> | </div> | ||
− | |||
+ | <footer id="footer"> | ||
+ | <div class="container" style="text-align: left"> | ||
+ | <h3 class="col-xs-12"><strong>Acknowledgments:</strong></h3> | ||
+ | <img style="max-width: 100%; height:auto; display: block;" src="https://static.igem.org/mediawiki/2017/8/88/2017_HUST_China_seven_logo.png" /> | ||
+ | </div> | ||
+ | </footer> | ||
+ | <script type="text/javascript" src="https://2017.igem.org/Team:HUST-China/js/bootstrap?action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="https://2017.igem.org/Team:HUST-China/js/jquery?action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="https://2017.igem.org/Team:HUST-China/js/return_top_jquery?action=raw&ctype=text/javascript"></script> | ||
+ | <script src="https://2017.igem.org/Team:HUST-China/js/return_top_script?action=raw&ctype=text/javascript" type="text/javascript"></script> | ||
+ | <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4/jquery.min.js"></script> | ||
+ | <script type="text/javascript" src="https://2017.igem.org/Team:HUST-China/ajax/libs/jquery/1.4/jquery.min.js?action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="https://2017.igem.org/Team:HUST-China/js/picbox.js?action=raw&ctype=text/javascript"></script> | ||
Latest revision as of 18:00, 1 November 2017