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} | } | ||
− | @media screen and (max-width: | + | @media screen and (max-width: 992px) { |
#section1, #section2, #section3, #section4, #section5 { | #section1, #section2, #section3, #section4, #section5 { | ||
margin-left: 50px; background-color: #eee; color:#171717; | margin-left: 50px; background-color: #eee; color:#171717; | ||
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body { | body { | ||
− | + | position: relative; | |
} | } | ||
+ | @media (min-width: 768px) { | ||
.menu_logo_1 { | .menu_logo_1 { | ||
− | + | top: 18px; | |
− | + | left:10px; | |
− | + | z-index: 1000; | |
− | + | position: fixed; | |
− | + | ||
} | } | ||
.menu_logo_2 { | .menu_logo_2 { | ||
− | + | top: 18px; | |
− | + | left:100px; | |
− | + | z-index: 1000; | |
− | + | position: fixed; | |
− | + | } | |
+ | .nav-pills{ | ||
+ | font-size:8px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 中等屏幕(桌面显示器,大于等于992px) */ | ||
+ | @media (min-width: 992px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:20px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:110px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:10px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 大屏幕(大桌面显示器,大于等于1200px) */ | ||
+ | @media (min-width: 1200px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:80px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:170px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:12px; | ||
+ | font-weight: bold; | ||
+ | } | ||
} | } | ||
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<body data-spy="scroll" data-target="#myScrollspy" data-offset="20"> | <body data-spy="scroll" data-target="#myScrollspy" data-offset="20"> | ||
− | + | <nav class="navbar navbar-default navbar-fixed-top" style="margin-top:15px"> | |
+ | <div class="container"> | ||
+ | <div class="navbar-header"> | ||
+ | <a href="https://2017.igem.org/Team:HUST-China" class="navbar-brand logo menu_logo_1"><img src="https://static.igem.org/mediawiki/2017/6/64/Team_HUST_China_2017_logo.png" alt="HUST_iGEM_2017_logo"></a> | ||
+ | <a href="https://2017.igem.org" class="navbar-brand logo menu_logo_2"><img src="https://static.igem.org/mediawiki/2017/f/f6/IGEM_2017_logo.png" alt="HUST_iGEM_2017_logo"></a> | ||
+ | <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#navbar-collapse" style="background-color: #333; color:#111"> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | </button> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="collapse navbar-collapse" id="navbar-collapse"> | ||
+ | <ul class="nav navbar-nav navbar-right"> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China">HOME</a></li> | ||
+ | <li class="dropdown active"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
+ | PROJECT <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/project/background">Background</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Description">Description</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Safety">Safety</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Demonstrate">Demonstrate</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
+ | WETLAB <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class<nav class="navbar navbar-default navbar-fixed-top" style="margin-top:15px"> | ||
<div class="container"> | <div class="container"> | ||
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<div class="collapse navbar-collapse" id="navbar-collapse"> | <div class="collapse navbar-collapse" id="navbar-collapse"> | ||
<ul class="nav navbar-nav navbar-right"> | <ul class="nav navbar-nav navbar-right"> | ||
− | <li | + | <li><a href="https://2017.igem.org/Team:HUST-China">HOME</a></li> |
<li class="dropdown"> | <li class="dropdown"> | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown"> | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
− | <li class="dropdown"> | + | <li class="dropdown active"> |
<a href="#" class="dropdown-toggle" data-toggle="dropdown"> | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | ||
WETLAB <b class="caret"></b> | WETLAB <b class="caret"></b> | ||
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</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
− | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | |
− | PARTS | + | PARTS<b class="caret"></b> |
</a> | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Basic part">Basic part</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Composite part">Composite part</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Part collection">Part collection</a></li> | ||
+ | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
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<li><a href="https://2017.igem.org/Team:HUST-China/Collaborations"> Collaborations</a></li> | <li><a href="https://2017.igem.org/Team:HUST-China/Collaborations"> Collaborations</a></li> | ||
<li><a href="https://2017.igem.org/Team:HUST-China/HP/Silver">HP/Silver</a></li> | <li><a href="https://2017.igem.org/Team:HUST-China/HP/Silver">HP/Silver</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:HUST-China/Engagement">Engagement</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
Line 413: | Line 495: | ||
</div> | </div> | ||
</nav> | </nav> | ||
− | |||
− | |||
<div class="tab1" id="section0" style="width:100%;margin-top: 65px;"> | <div class="tab1" id="section0" style="width:100%;margin-top: 65px;"> | ||
Line 432: | Line 512: | ||
<li><a href="#section5">Calibration Protocol</a></li> | <li><a href="#section5">Calibration Protocol</a></li> | ||
<li><a href="#section6">Cell Culture</a></li> | <li><a href="#section6">Cell Culture</a></li> | ||
− | <li><a href="#section7">Interlab | + | <li><a href="#section7">Interlab Results</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
Line 446: | Line 526: | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Provenance and Release</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Provenance and Release</h3> | ||
<h4><strong>①Individuals responsible for conducting InterLab study</strong> </h4> | <h4><strong>①Individuals responsible for conducting InterLab study</strong> </h4> | ||
− | <div class="table-responsive" style="padding: 10px 100px; text-align: center;"> | + | <div class="table-responsive" style="padding: 10px 100px; text-align: center;font-size:14px;"> |
<table class="table table-hover"> | <table class="table table-hover"> | ||
<thead> | <thead> | ||
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</div> | </div> | ||
<h4><strong> ②Corresponding email</strong> </h4> | <h4><strong> ②Corresponding email</strong> </h4> | ||
− | <div class="table-responsive" style="padding: 10px 100px; text-align: center;"> | + | <div class="table-responsive" style="padding: 10px 100px; text-align: center;font-size:14px;"> |
<table class="table table-hover"> | <table class="table table-hover"> | ||
<thead> | <thead> | ||
Line 486: | Line 566: | ||
<tr> | <tr> | ||
<td>Kangyuan Yu</td> | <td>Kangyuan Yu</td> | ||
− | <td> | + | <td>u201612160@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Haibo Huang </td> | <td>Haibo Huang </td> | ||
− | <td> | + | <td>u201612177@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Ziyang Xiao</td> | <td>Ziyang Xiao</td> | ||
− | <td> | + | <td>u201612166@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Shaofeng Liao</td> | <td>Shaofeng Liao</td> | ||
− | <td> | + | <td>u201612159@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>HuiPing Shi</td> | <td>HuiPing Shi</td> | ||
− | <td> | + | <td>u201513154@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 515: | Line 595: | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | ||
<h4><strong> What chassis did you use?</strong> </h4> | <h4><strong> What chassis did you use?</strong> </h4> | ||
− | <p>Escherichia coli | + | <p>Escherichia coli DH5α</p> |
<h4><strong> What Biosafety Level is your chassis? </strong> </h4> | <h4><strong> What Biosafety Level is your chassis? </strong> </h4> | ||
<p>BSL1</p> | <p>BSL1</p> | ||
Line 558: | Line 638: | ||
<li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | <li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | ||
<li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> | <li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
Line 596: | Line 675: | ||
<li>③ Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li> | <li>③ Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li> | ||
<li>④ *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li> | <li>④ *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
Line 606: | Line 684: | ||
<div class="col-xs-12" > | <div class="col-xs-12" > | ||
<h5><strong> Part 2: Prepare the serial dilutions of Fluorescein </strong> </h5> | <h5><strong> Part 2: Prepare the serial dilutions of Fluorescein </strong> </h5> | ||
− | < | + | <ul style="list-style: none; font-size:10px;"> |
<li>①Add 100 μl of 1xPBS into wells A2, B2, C2, D2....A12, B12, C12, D12.</li> | <li>①Add 100 μl of 1xPBS into wells A2, B2, C2, D2....A12, B12, C12, D12.</li> | ||
<li>②Add 200 μl of 1x Fluorescein stock solution into A1, B1, C1, D1.</li> | <li>②Add 200 μl of 1x Fluorescein stock solution into A1, B1, C1, D1.</li> | ||
Line 620: | Line 698: | ||
<li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | <li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | ||
<li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | <li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | ||
− | <li>⑭ | + | <li>⑭ Take care not to continue serial dilution into column 12.</li> |
<li>⑮ Repeat dilution series for rows B, C, D. | <li>⑮ Repeat dilution series for rows B, C, D. | ||
<li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | <li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | ||
− | </ | + | </ul> |
</div> | </div> | ||
Line 670: | Line 748: | ||
<li>① Set your instrument to read Abs600 (as OD calibration setting)</li> | <li>① Set your instrument to read Abs600 (as OD calibration setting)</li> | ||
<li>② Measure Abs600 of the overnight cultures</li> | <li>② Measure Abs600 of the overnight cultures</li> | ||
− | <li>③ | + | <li>③ Dilute the cultures to a target Abs600 of 0.02 (see the volume of preloading culture and media in Excel Dilution Calculation sheets) in 12 ml LB medium + Chloramphenicol in 50 mL falcon tube.</li> |
− | + | <li>④ Remove 500uL of each culture for your zero time point and hold these samples on ice. (You should have 16 sample tubes from the time=0 time point)</li> | |
− | <li> | + | <li>⑤ Incubate the cultures at 37°C and 220 rpm.</li> |
− | <li> | + | <li>⑥ Remove 500uL samples of each culture after 2, 4 and 6 hours of growth. Keep samples on ice while completing the additional time points. You should have 16 sample tubes for each time point.</li> |
− | <li> | + | <li>⑦ Set up your four measurement plates: For colony #1 culture, pipet 100 uL samples into wells A, B, C and D of the row for that device. For colony #2, pipet 100uL samples into wells E, F, G, and H of the row for that device.</li> |
− | <li> | + | <li>⑧ Read your plates, taking care to use the exact same settings used for your fluorescein measurement.</li> |
− | <li> | + | |
Line 698: | Line 775: | ||
<ul class="yuandian"> | <ul class="yuandian"> | ||
<li>Measure OD and fluorescence of all samples</li> | <li>Measure OD and fluorescence of all samples</li> | ||
− | |||
</ul> | </ul> | ||
<h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> | <h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> |
Latest revision as of 18:00, 1 November 2017