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} | } | ||
− | @media screen and (max-width: | + | @media screen and (max-width: 992px) { |
#section1, #section2, #section3, #section4, #section5 { | #section1, #section2, #section3, #section4, #section5 { | ||
margin-left: 50px; background-color: #eee; color:#171717; | margin-left: 50px; background-color: #eee; color:#171717; | ||
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body { | body { | ||
− | + | position: relative; | |
} | } | ||
+ | @media (min-width: 768px) { | ||
.menu_logo_1 { | .menu_logo_1 { | ||
− | + | top: 18px; | |
− | + | left:10px; | |
− | + | z-index: 1000; | |
− | + | position: fixed; | |
− | + | ||
} | } | ||
.menu_logo_2 { | .menu_logo_2 { | ||
− | + | top: 18px; | |
− | + | left:100px; | |
− | + | z-index: 1000; | |
− | + | position: fixed; | |
− | + | } | |
+ | .nav-pills{ | ||
+ | font-size:8px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 中等屏幕(桌面显示器,大于等于992px) */ | ||
+ | @media (min-width: 992px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:20px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:110px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:10px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 大屏幕(大桌面显示器,大于等于1200px) */ | ||
+ | @media (min-width: 1200px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:80px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:170px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:12px; | ||
+ | font-weight: bold; | ||
+ | } | ||
} | } | ||
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<li><a href="#section5">Calibration Protocol</a></li> | <li><a href="#section5">Calibration Protocol</a></li> | ||
<li><a href="#section6">Cell Culture</a></li> | <li><a href="#section6">Cell Culture</a></li> | ||
− | <li><a href="#section7">Interlab | + | <li><a href="#section7">Interlab Results</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
Line 522: | Line 566: | ||
<tr> | <tr> | ||
<td>Kangyuan Yu</td> | <td>Kangyuan Yu</td> | ||
− | <td> | + | <td>u201612160@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Haibo Huang </td> | <td>Haibo Huang </td> | ||
− | <td> | + | <td>u201612177@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Ziyang Xiao</td> | <td>Ziyang Xiao</td> | ||
− | <td> | + | <td>u201612166@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Shaofeng Liao</td> | <td>Shaofeng Liao</td> | ||
− | <td> | + | <td>u201612159@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>HuiPing Shi</td> | <td>HuiPing Shi</td> | ||
− | <td> | + | <td>u201513154@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | ||
<h4><strong> What chassis did you use?</strong> </h4> | <h4><strong> What chassis did you use?</strong> </h4> | ||
− | <p>Escherichia coli | + | <p>Escherichia coli DH5α</p> |
<h4><strong> What Biosafety Level is your chassis? </strong> </h4> | <h4><strong> What Biosafety Level is your chassis? </strong> </h4> | ||
<p>BSL1</p> | <p>BSL1</p> | ||
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<li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | <li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | ||
<li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> | <li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
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<li>③ Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li> | <li>③ Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li> | ||
<li>④ *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li> | <li>④ *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
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<div class="col-xs-12" > | <div class="col-xs-12" > | ||
<h5><strong> Part 2: Prepare the serial dilutions of Fluorescein </strong> </h5> | <h5><strong> Part 2: Prepare the serial dilutions of Fluorescein </strong> </h5> | ||
− | < | + | <ul style="list-style: none; font-size:10px;"> |
<li>①Add 100 μl of 1xPBS into wells A2, B2, C2, D2....A12, B12, C12, D12.</li> | <li>①Add 100 μl of 1xPBS into wells A2, B2, C2, D2....A12, B12, C12, D12.</li> | ||
<li>②Add 200 μl of 1x Fluorescein stock solution into A1, B1, C1, D1.</li> | <li>②Add 200 μl of 1x Fluorescein stock solution into A1, B1, C1, D1.</li> | ||
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<li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | <li>⑫ Mix A10 by pipetting up and down 3x and transfer 100 μl into A11.</li> | ||
<li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | <li>⑬ Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.</li> | ||
− | <li>⑭ | + | <li>⑭ Take care not to continue serial dilution into column 12.</li> |
<li>⑮ Repeat dilution series for rows B, C, D. | <li>⑮ Repeat dilution series for rows B, C, D. | ||
<li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | <li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | ||
− | </ | + | </ul> |
</div> | </div> | ||
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<li>① Set your instrument to read Abs600 (as OD calibration setting)</li> | <li>① Set your instrument to read Abs600 (as OD calibration setting)</li> | ||
<li>② Measure Abs600 of the overnight cultures</li> | <li>② Measure Abs600 of the overnight cultures</li> | ||
− | <li>③ | + | <li>③ Dilute the cultures to a target Abs600 of 0.02 (see the volume of preloading culture and media in Excel Dilution Calculation sheets) in 12 ml LB medium + Chloramphenicol in 50 mL falcon tube.</li> |
− | + | <li>④ Remove 500uL of each culture for your zero time point and hold these samples on ice. (You should have 16 sample tubes from the time=0 time point)</li> | |
− | <li> | + | <li>⑤ Incubate the cultures at 37°C and 220 rpm.</li> |
− | <li> | + | <li>⑥ Remove 500uL samples of each culture after 2, 4 and 6 hours of growth. Keep samples on ice while completing the additional time points. You should have 16 sample tubes for each time point.</li> |
− | <li> | + | <li>⑦ Set up your four measurement plates: For colony #1 culture, pipet 100 uL samples into wells A, B, C and D of the row for that device. For colony #2, pipet 100uL samples into wells E, F, G, and H of the row for that device.</li> |
− | <li> | + | <li>⑧ Read your plates, taking care to use the exact same settings used for your fluorescein measurement.</li> |
− | <li> | + | |
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<ul class="yuandian"> | <ul class="yuandian"> | ||
<li>Measure OD and fluorescence of all samples</li> | <li>Measure OD and fluorescence of all samples</li> | ||
− | |||
</ul> | </ul> | ||
<h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> | <h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> |
Latest revision as of 18:00, 1 November 2017