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} | } | ||
− | @media screen and (max-width: | + | @media screen and (max-width: 992px) { |
#section1, #section2, #section3, #section4, #section5 { | #section1, #section2, #section3, #section4, #section5 { | ||
margin-left: 50px; background-color: #eee; color:#171717; | margin-left: 50px; background-color: #eee; color:#171717; | ||
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body { | body { | ||
− | + | position: relative; | |
} | } | ||
+ | @media (min-width: 768px) { | ||
.menu_logo_1 { | .menu_logo_1 { | ||
− | + | top: 18px; | |
− | + | left:10px; | |
− | + | z-index: 1000; | |
− | + | position: fixed; | |
− | + | ||
} | } | ||
.menu_logo_2 { | .menu_logo_2 { | ||
− | + | top: 18px; | |
− | + | left:100px; | |
− | + | z-index: 1000; | |
− | + | position: fixed; | |
− | + | } | |
+ | .nav-pills{ | ||
+ | font-size:8px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 中等屏幕(桌面显示器,大于等于992px) */ | ||
+ | @media (min-width: 992px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:20px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:110px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:10px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 大屏幕(大桌面显示器,大于等于1200px) */ | ||
+ | @media (min-width: 1200px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:80px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:170px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:12px; | ||
+ | font-weight: bold; | ||
+ | } | ||
} | } | ||
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<li><a href="#section5">Calibration Protocol</a></li> | <li><a href="#section5">Calibration Protocol</a></li> | ||
<li><a href="#section6">Cell Culture</a></li> | <li><a href="#section6">Cell Culture</a></li> | ||
− | <li><a href="#section7">Interlab | + | <li><a href="#section7">Interlab Results</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div id="section1" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | <div id="section1" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Introduction</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Introduction</h3> | ||
− | <p style="padding:10px">It is significant for synthetic biology to develop a reliable and repeatable measurement, the same to all the other engineering disciplines. We HUST-China have volunteered to test some RBS devices (BCDs) that are intended to make gene expression more precise and reliable by | + | <p style="padding:10px">It is significant for synthetic biology to develop a reliable and repeatable measurement, the same to all the other engineering disciplines. We HUST-China have volunteered to test some RBS devices (BCDs) that are intended to make gene expression more precise and reliable by measure the expression level of GFP, in order to help the iGEM community collect data about how reliable will these devices turn out to be in labs around the world. |
</p> | </p> | ||
</div> | </div> | ||
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<tr> | <tr> | ||
<td>Kangyuan Yu</td> | <td>Kangyuan Yu</td> | ||
− | <td> | + | <td>u201612160@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Haibo Huang </td> | <td>Haibo Huang </td> | ||
− | <td> | + | <td>u201612177@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Ziyang Xiao</td> | <td>Ziyang Xiao</td> | ||
− | <td> | + | <td>u201612166@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Shaofeng Liao</td> | <td>Shaofeng Liao</td> | ||
− | <td> | + | <td>u201612159@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>HuiPing Shi</td> | <td>HuiPing Shi</td> | ||
− | <td> | + | <td>u201513154@hust.edu.cn</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3> | ||
<h4><strong> What chassis did you use?</strong> </h4> | <h4><strong> What chassis did you use?</strong> </h4> | ||
− | <p>Escherichia coli | + | <p>Escherichia coli DH5α</p> |
<h4><strong> What Biosafety Level is your chassis? </strong> </h4> | <h4><strong> What Biosafety Level is your chassis? </strong> </h4> | ||
<p>BSL1</p> | <p>BSL1</p> | ||
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<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Instrument</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Instrument</h3> | ||
<h4><strong> What instrument did you use during your measurements? </strong> </h4> | <h4><strong> What instrument did you use during your measurements? </strong> </h4> | ||
− | <p> | + | <p>plate reader</p> |
<h4><strong> Please provide the brand and model of your instrument.</strong> </h4> | <h4><strong> Please provide the brand and model of your instrument.</strong> </h4> | ||
<p>Flexstation 3</p> | <p>Flexstation 3</p> | ||
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<li>②Add 100 μl LUDOX 100 % into wells A1, B1, C1, D1</li> | <li>②Add 100 μl LUDOX 100 % into wells A1, B1, C1, D1</li> | ||
<li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | <li>③Add 100 μl of dH2O into A2, B2, C2, D2</li> | ||
− | <li>④Measure absorbance 600 nm of all samples in all standard measurement | + | <li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <h4><strong> B1. Protocol for | + | <h4><strong> B1. Protocol for FIuorescein Fluoresence standard curve</strong> </h4> |
<h5><strong>Did you use pathlength correction during measurement? </strong> </h5> | <h5><strong>Did you use pathlength correction during measurement? </strong> </h5> | ||
<p>Yes</p> | <p>Yes</p> | ||
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<div class="col-xs-12" > | <div class="col-xs-12" > | ||
<h5><strong> Part 2: Prepare the serial dilutions of Fluorescein </strong> </h5> | <h5><strong> Part 2: Prepare the serial dilutions of Fluorescein </strong> </h5> | ||
− | < | + | <ul style="list-style: none; font-size:10px;"> |
<li>①Add 100 μl of 1xPBS into wells A2, B2, C2, D2....A12, B12, C12, D12.</li> | <li>①Add 100 μl of 1xPBS into wells A2, B2, C2, D2....A12, B12, C12, D12.</li> | ||
<li>②Add 200 μl of 1x Fluorescein stock solution into A1, B1, C1, D1.</li> | <li>②Add 200 μl of 1x Fluorescein stock solution into A1, B1, C1, D1.</li> | ||
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<li>⑮ Repeat dilution series for rows B, C, D. | <li>⑮ Repeat dilution series for rows B, C, D. | ||
<li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | <li>⑯ Measure fluorescence of all samples in all standard measurement modes in instrument. | ||
− | </ | + | </ul> |
</div> | </div> | ||
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<ul class="yuandian"> | <ul class="yuandian"> | ||
<li>Measure OD and fluorescence of all samples</li> | <li>Measure OD and fluorescence of all samples</li> | ||
− | |||
</ul> | </ul> | ||
<h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> | <h5><strong>Suggested Plate Layout for 96-well Plate</strong> </h5> | ||
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</div> | </div> | ||
<div id="section7" style="border: solid 1px #666; margin:5px; padding:5px;border-radius:10px;overflow: hidden;"> | <div id="section7" style="border: solid 1px #666; margin:5px; padding:5px;border-radius:10px;overflow: hidden;"> | ||
− | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Interlab | + | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Interlab Resultn</h3> |
<h5 class="col-xs-12"><strong>OD600 Reference Point</strong> </h5> | <h5 class="col-xs-12"><strong>OD600 Reference Point</strong> </h5> | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/5/52/2017_HUST_China_interlab_img_table2.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/5/52/2017_HUST_China_interlab_img_table2.png" alt="demo1" class="col-xs-8 col-xs-offset-2"> |
Latest revision as of 18:00, 1 November 2017